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Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Raw264.7 Cells (30ug); Lane 2: Mouse Liver (40ug); Probed with IL-1 Beta Polyclonal Antibody, unconjugated (bs-6319R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Raw264.7 cell lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1 Beta Polyclonal Antibody(bs-6319R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6319R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0778R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: PE

Catalog #1: bs-6319R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: HRP

Catalog #1: bs-0778R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: FITC

Catalog #1: bs-0778R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Biotin

Catalog #1: bs-6319R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0756R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others, Monkey

Conjugate: PE-Cy5.5

Catalog #1: bs-6319R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy5

Catalog #1: bs-6319R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Biotin

Catalog #1: bs-0778R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Cy7

Catalog #1: bs-1992R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: PE

Catalog #1: bs-6319R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy3

Catalog #1: bs-6319R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy7

Catalog #1: bs-6319R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: FITC

Catalog #1: bs-0778R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Cy3

Catalog #1: bs-0778R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Cy5

Catalog #1: bs-0778R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: HRP

Catalog #1: bs-1019R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: Biotin

Catalog #1: bs-1019R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: HRP

Catalog #1: bs-0465R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Cy5.5

Catalog #1: bs-2993R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Cy5.5

Catalog #1: bs-0115R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy5.5

Catalog #1: bs-0876R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy5.5

Catalog #1: bs-0081R-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Cy5.5

Catalog #1: bs-1172R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: Cy5.5

Catalog #1: bs-1021R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Cy5.5

Catalog #1: bs-1309R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Cy5.5

Catalog #1: bs-0778R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Cy5.5

Catalog #1: bs-0756R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others, Monkey

Conjugate: Cy5.5

Catalog #1: bs-1712R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Cy5.5

Catalog #1: bs-0127R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Rabbit, Zebrafish

Conjugate: Cy5.5

Catalog #1: bs-1992R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: Cy5.5

Catalog #1: bs-2202R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine

Conjugate: Cy5.5

Catalog #1: bs-0081R-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: PE

Catalog #1: bs-1309R-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: PE

Catalog #1: bs-1712R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: PE

Catalog #1: bs-1019R-Cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: Cy5

Catalog #1: bs-1019R-FITC

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: FITC

Catalog #1: bs-1019R-Cy3

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: Cy3

Catalog #1: bs-1019R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: PE

Catalog #1: bs-1019R-Cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: Cy7

Catalog #1: bs-1019R-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: Cy5.5

Catalog #1: bs-0115R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: PE

Catalog #1: bs-1021R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: PE

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Raw264.7 Cells (30ug); Lane 2: Mouse Liver (40ug); Probed with IL-1 Beta Polyclonal Antibody, unconjugated (bs-6319R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Raw264.7 cell lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1 Beta Polyclonal Antibody(bs-6319R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6319R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-6319R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Biotin

Catalog #1: bs-6319R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: HRP

Catalog #1: bs-6319R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy7