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Cardiac Markers

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Catalog #1: bs-1618R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-1618R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-1019R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: Biotin

Catalog #1: bs-1019R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Conjugate: HRP

Human heart lysates probed with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human heart tissue incubated with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52818R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

HL-60 cells lysates probed with Myeloperoxidase (3C3) Monoclonal Antibody (bsm-52896R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Myeloperoxidase (3C3) Monoclonal Antibody (bsm-52896R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Myeloperoxidase (3C3) Monoclonal Antibody (bsm-52896R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Myeloperoxidase (3C3) Monoclonal Antibody (bsm-52896R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52896R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Human skeletal muscle lysate probed with Myoglobin (2D4) Monoclonal Antibody (bsm-52526R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Myoglobin (2D4) Monoclonal Antibody (bsm-52526R) at 1:100 in C2C12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with Myoglobin (2D4) Monoclonal Antibody (bsm-52526R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Myoglobin (2D4) Monoclonal Antibody (bsm-52526R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52526R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1004R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-21310R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-21310R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-0391M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-0391M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRP (C11F2) Monoclonal Antibody, Unconjugated (bsm-0391M) at 1:400 overnight at 4\u00b0C, followed secondary and DAB staining.
Lane 1: C Reactive Protein (CRP); 40ug loaded into the lane; Probed with CRP (C11F2) Monoclonal Antibody, unconjugated (bsm-0391M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: C Reactive Protein(CRP) probed with CRP(C11F2) Monoclona Antibody, Unconjugated (bsm-0391M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U-2OS cell lysates; Lane 2: Human Siha cell lysates probed with CRP(C11F2) Monoclonal Antibody, Unconjugated bsm-0391MR) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0391M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated