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CD Markers

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Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\\n
Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.

Catalog #1: bs-0647R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse heart tissue labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution for 40 minutes at 37\u00b0C
Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:3200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0646R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining.
Lane 1: Rat Thymus lysates; Lane 2: Rat lymph node lysates probed with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4791R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-0646R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: FITC

Formalin-fixed and paraffin embedded chicken ileum labeled with Anti-CD44V6 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:300 followed by conjugation to the secondary antibody
Rat lung lysate probed with Anti CD44 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 80kD. Observed band size: 80kD
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD44 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD44 Polyclonal Antibody(bs-2507R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2507R

Applications: ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-NCAM1 Polyclonal Antibody, Unconjugated (bs-0736R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Human HeLa lysates probed with Rabbit Anti-CD56 Polyclonal Antibody, Unconjugated (bs-0736R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0736R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-0646R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: PE

Catalog #1: bs-0646R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: Cy3

Catalog #1: bs-0646R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: Biotin

Catalog #1: bs-0646R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: HRP

Catalog #1: bs-0647R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Biotin

Catalog #1: bs-0736R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy3

Catalog #1: bs-0647R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: AbBy Fluor® 488

Catalog #1: bs-4791R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0646R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: Cy5

Catalog #1: bs-0646R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: Cy7

Catalog #1: bs-0736R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Chicken

Conjugate: HRP

Catalog #1: bs-0736R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy5

Catalog #1: bs-0736R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: FITC

Catalog #1: bs-2507R-FITC

Applications: FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: FITC

Catalog #1: bs-4791R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Chicken

Conjugate: Biotin

Catalog #1: bs-0647R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Cy3

Catalog #1: bs-0647R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Cy5

Catalog #1: bs-0647R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Cy7

Catalog #1: bs-0647R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: FITC

Catalog #1: bs-0647R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: HRP

Catalog #1: bs-0736R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Chicken

Conjugate: Biotin

Catalog #1: bs-0736R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy7

Catalog #1: bs-2507R-Cy3

Applications: FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy3

Catalog #1: bs-2507R-Cy5

Applications: FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy5

Catalog #1: bs-2507R-Cy7

Applications: FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy7

Catalog #1: bs-2507R-Biotin

Applications: ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Biotin

Catalog #1: bs-2507R-HRP

Applications: ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: HRP

Catalog #1: bs-4791R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy3

Catalog #1: bs-4791R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy5

Catalog #1: bs-4791R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy7

Catalog #1: bs-4791R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: FITC

Catalog #1: bs-4791R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Chicken

Conjugate: HRP

Catalog #1: bs-0647R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: PE

Catalog #1: bs-0646R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: Cy5.5

Catalog #1: bs-0736R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy5.5

Catalog #1: bs-0647R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Cy5.5

Catalog #1: bs-2507R-Cy5.5

Applications: FCM, IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy5.5

Catalog #1: bs-4791R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy5.5

Catalog #1: bs-0736R-PE

Applications: WB, FCM

Reactivity: Human, Rat, Chicken

Conjugate: PE

Catalog #1: bs-2507R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: PE

Catalog #1: bs-4791R-PE

Applications: WB, FCM

Reactivity: Human, Rat, Chicken

Conjugate: PE

Catalog #1: bs-0646R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: AbBy Fluor® 350