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Formalin-fixed and paraffin-embedded rat colon labeled with Rabbit Anti-CD163\/M130 Polyclonal Antibody, Unconjugated( bs-2527R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163\/M130 Polyclonal Antibody, Unconjugated (bs-2527R) followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse uterus lysates probed (bs-2527R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 130kDa.
Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163\/M130 Polyclonal Antibody, unconjugated (bs-2527R) at 1:100 for 40 minutes at 37\u00b0C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37\u00b0C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative. \\n

Catalog #1: bs-2527R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1\/CD90\/ Thy1.1 Polyclonal Antibody, Unconjugated (bs-0778R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with CD90 Polyclonal Antibody, Unconjugated (bs-0778R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0778R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse kidney cells labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0766R) 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded Lymphocytes labeled with (bs-0766R) Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated followed by incubation with a conjugated secondary antibody.
Formalin-fixed and paraffin embedded Lymphocytes labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) followed by incubation with a conjugated secondary antibody and DAPI staining.

Catalog #1: bs-0766R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Image kindly submitted by Jennifer DeMuro as part of the Free Sample Program. Mouse brain lysates probed with Rabbit Anti-Leptin receptor Polyclonal Antibody (bs-0961R).
K562 cells probed with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Liver; Probed with Leptin Receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0961R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Integrin beta 1\/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Integrin beta 1\/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human A431 cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Rat Ear lysates; Lane 4: Mouse Ear lysates; Lane 5: Mouse Urinary bladder lysates probed with Integrin beta 1 Polyclonal Antibody, Unconjugated (bs-0486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Integrin beta 1) polyclonal Antibody, Unconjugated (bs-0486R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0486R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Unconjugated

U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163\/M130 Polyclonal Antibody(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163\/M130 Polyclonal Antibody,FITC Conjugated(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2527R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: FITC

Catalog #1: bs-2527R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: PE

Catalog #1: bs-0961R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Others

Conjugate: PE

Catalog #1: bs-2527R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Cy5

Catalog #1: bs-2527R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Cy3

Catalog #1: bs-0486R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: FITC

Catalog #1: bs-0961R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: FITC

Catalog #1: bs-0961R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bs-0778R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: PE

Catalog #1: bs-2527R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Biotin

Catalog #1: bs-0778R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: FITC

Catalog #1: bs-0766R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bs-0778R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Biotin

Catalog #1: bs-2527R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Cy7

Catalog #1: bs-0961R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy7

Catalog #1: bs-0961R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

Catalog #1: bs-0766R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bs-0766R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: FITC

Catalog #1: bs-2527R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: HRP

Catalog #1: bs-0778R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Cy7

Catalog #1: bs-2527R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Cy5.5

Catalog #1: bs-0486R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy3

Catalog #1: bs-0486R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy5

Catalog #1: bs-0486R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy7

Catalog #1: bs-0486R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Biotin

Catalog #1: bs-0486R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: HRP

Catalog #1: bs-0961R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bs-0961R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: HRP

Catalog #1: bs-0766R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy7

Catalog #1: bs-0766R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

Catalog #1: bs-0766R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: HRP

Catalog #1: bs-0778R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Cy3

Catalog #1: bs-0778R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Cy5

Catalog #1: bs-0778R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: HRP

Catalog #1: bs-0486R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PE

Catalog #1: bs-0778R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Cy5.5

Catalog #1: bs-0766R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5.5

Catalog #1: bs-0486R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy5.5

Catalog #1: bs-0961R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5.5

Catalog #1: bs-0766R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Others

Conjugate: PE

Catalog #1: bs-0961R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0961R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0961R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 488