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Rat lung lysate 30ug, probed (bs-0061R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney cancer labeled with Anti-HIF-2\u03b1 Polyclonal Antibody, Unconjugated (bs-1447R) followed by conjugation to the secondary antibody and DAB staining
Rat lung lysates probed with Rabbit Anti-HIF2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 and Mouse Anti-GAPDH (bsm-0978M) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
HepG2 cells(black) were fixed with 70% ice-cold methanol overnight at 4\u2103,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HIF2 alpha Polyclonal Antibody(bs-1447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1447R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, Unconjugated (bs-0623R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0623R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: BS-0623R-PE-CY7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: PE

Catalog #1: bs-1447R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Cy3

Catalog #1: bs-1447R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Cy5

Catalog #1: bs-1447R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Cy7

Catalog #1: bs-1447R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: FITC

Catalog #1: bs-1447R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Biotin

Catalog #1: bs-1447R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: HRP

Catalog #1: bs-1447R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Cy5.5

Catalog #1: bs-0081R-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Cy5.5

Catalog #1: bs-0217R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0061R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Cy5.5

Catalog #1: bs-0052R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: Cy5.5

Catalog #1: bs-0633R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy5.5

Catalog #1: bs-0754R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Sheep, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0623R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Biotin

Catalog #1: bs-0623R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-0623R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Cy5

Catalog #1: bs-0623R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Cy3

Catalog #1: bs-0623R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0623R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Cy7

Catalog #1: bs-0623R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: FITC

Catalog #1: bs-0623R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: PE

Catalog #1: bs-1447R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: PE

Catalog #1: bs-0081R-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: PE

Catalog #1: bs-0217R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: PE

Catalog #1: bs-0061R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: PE

Catalog #1: bs-0052R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Sheep, Monkey

Conjugate: PE

Catalog #1: bs-0633R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: PE

Catalog #1: bs-0754R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Chicken, Sheep, Rabbit

Conjugate: PE

Catalog #1: bs-1447R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: AbBy Fluor® 350

Catalog #1: bs-1447R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: AbBy Fluor® 405

Catalog #1: bs-1447R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: AbBy Fluor® 488

Catalog #1: bs-1447R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: AbBy Fluor® 555

Catalog #1: bs-1447R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: AbBy Fluor® 594

Catalog #1: bs-1447R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: AbBy Fluor® 647

Catalog #1: bs-1447R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: AbBy Fluor® 680

Catalog #1: bs-1447R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: AbBy Fluor® 750

Catalog #1: bs-1447R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: APC

Catalog #1: bs-1447R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: APC-Cy5

Catalog #1: bs-1447R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: APC-Cy5.5

Catalog #1: bs-1447R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: APC-Cy7

Catalog #1: bs-1447R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: PE-Cy3

Catalog #1: bs-1447R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: PE-Cy5

Catalog #1: bs-1447R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: PE-Cy5.5

Catalog #1: bs-1447R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: PE-Cy7