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\"Antigen: bs-1240P Hu Fibrinogen, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Mouse IgG (bs-0296G-HRP) at 1: 5000; \\nRead the data in MicroplateReader by 450nm. \\n\"
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with human Fibrinogen Monoclonal Antibody(bsm-1240M)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-1240M

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Others

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A549 lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0945M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-0945M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-51054M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-54472R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-1240M-FITC

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: FITC

Catalog #1: bsm-1240M-Biotin

Applications: IHC-P

Reactivity: Human, Others

Conjugate: Biotin

Lane 1: A431; Lane 2: PC-12; Lane 3: NIH\/3T3 cell lysate (20\u00b5g per lane) probed with bsm-51339M MITF (M2A1) Monoclonal Antibody at 1:2000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: A431 cell lysates probed with MITF (M2A1) Monoclona Antibody, Unconjugated (bsm-51339M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51339M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-10855M-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0945M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-0945M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-0945M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-0945M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-0945M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-1240M-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy3

Catalog #1: bsm-1240M-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy5

Catalog #1: bsm-1240M-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy7

Catalog #1: bsm-1240M-HRP

Applications: IHC-P

Reactivity: Human, Others

Conjugate: HRP

Catalog #1: bsm-51192M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MDA-MB435 Cells; 35ug loaded into the lane; Probed with MUC1 (M5C7) Monoclonal Antibody, Unconjugated (bsm-51126M) at 1:200 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MUC1 Monoclonal Antibody(bsm-51226M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MUC1) polyclonal Antibody, Unconjugated (bsm-51126M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-51126M

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

NIH-3T3 cells lysates probed with Syndecan 1 (4C7) Monoclonal Antibody (bsm-52917R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast cancer tissue incubated with Syndecan 1 (4C7) Monoclonal Antibody (bsm-52917R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with Syndecan 1 (4C7) Monoclonal Antibody (bsm-52917R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin

Catalog #1: bsm-52917R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of MCF7 cells with MUC1 (5G5) Monoclonal Antibody (bsm-52576R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Hela cells lysate probed with MUC1 (5G5) Monoclonal Antibody (bsm-52576R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MUC1 (5G5) Monoclonal Antibody (bsm-52576R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MUC1 (5G5) Monoclonal Antibody (bsm-52576R) at 1:100 in B16-F1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52576R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Desmoglein-1 (5C11) Monoclonal Antibody (bsm-54326R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with Desmoglein-1 (5C11) Monoclonal Antibody (bsm-54326R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Uterus tissue incubated with Desmoglein-1 (5C11) Monoclonal Antibody (bsm-54326R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Skin tissue incubated with Desmoglein-1 (5C11) Monoclonal Antibody (bsm-54326R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54326R

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: full-length PD-L1 transfected 293T; Lane 2: untransfected 293T, probed with PD-L1 (6C8) Monoclonal Antibody, Unconjugated (bsm-58003R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Flow cytometric analysis of 293F cells expressing PD-L1 probed with PD-L1 (6C8) Monoclonal Antibody, unconjugated (bsm-58003R) at 1:100 dilution (red), compared control IgG staining (blue).

Catalog #1: bsm-58003R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Galectin 3 (2C7) Monoclonal Antibody (bsm-52828R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela lysates; Lane 2: NIH\/3T3 lysates; Line 3: THP-1 lysates ;Probed with Galectin 3 (2C7) Monoclonal Antibody (bsm-52828R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Galectin 3 (2C7) Monoclonal Antibody (bsm-52828R) at 1:100 in NIH\/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52828R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0945M-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-1240M-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy5.5

Catalog #1: bsm-0391M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-0391M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRP (C11F2) Monoclonal Antibody, Unconjugated (bsm-0391M) at 1:400 overnight at 4\u00b0C, followed secondary and DAB staining.
Lane 1: C Reactive Protein (CRP); 40ug loaded into the lane; Probed with CRP (C11F2) Monoclonal Antibody, unconjugated (bsm-0391M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: C Reactive Protein(CRP) probed with CRP(C11F2) Monoclona Antibody, Unconjugated (bsm-0391M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U-2OS cell lysates; Lane 2: Human Siha cell lysates probed with CRP(C11F2) Monoclonal Antibody, Unconjugated bsm-0391MR) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0391M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-10855M

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-10855M-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-33248M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-33248M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-33248M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33248M

Applications: IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33248M-Biotin

Applications: IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33248M-HRP

Applications: IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-0391M-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-0391M-Cy7

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-0391M-Cy5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-0391M-Cy3

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-0391M-FITC

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-0391M-PE

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-0391M-BF350

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-0391M-BF405

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-0391M-BF488

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-0391M-BF555

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-0391M-BF594

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-0391M-BF647

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-0391M-BF680

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680