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\"Antigen: bs-1240P Hu Fibrinogen, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Mouse IgG (bs-0296G-HRP) at 1: 5000; \\nRead the data in MicroplateReader by 450nm. \\n\"
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with human Fibrinogen Monoclonal Antibody(bsm-1240M)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-1240M

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Others

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A549 lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0945M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-0945M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-51054M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-1240M-FITC

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: FITC

Catalog #1: bsm-1240M-Biotin

Applications: IHC-P

Reactivity: Human, Others

Conjugate: Biotin

Lane 1: A431; Lane 2: PC-12; Lane 3: NIH\/3T3 cell lysate (20\u00b5g per lane) probed with bsm-51339M MITF (M2A1) Monoclonal Antibody at 1:2000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: A431 cell lysates probed with MITF (M2A1) Monoclona Antibody, Unconjugated (bsm-51339M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51339M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0945M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-0945M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-0945M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-0945M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-0945M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-1240M-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy3

Catalog #1: bsm-1240M-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy5

Catalog #1: bsm-1240M-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy7

Catalog #1: bsm-1240M-HRP

Applications: IHC-P

Reactivity: Human, Others

Conjugate: HRP

Catalog #1: bsm-51192M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0945M-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-1240M-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy5.5

Catalog #1: bsm-0945M-PE

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-0945M-BF350

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-0945M-BF405

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-0945M-BF488

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-0945M-BF555

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-0945M-BF594

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-0945M-BF647

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-0945M-BF680

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-0945M-BF750

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-0945M-APC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-0945M-APC-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy5

Catalog #1: bsm-0945M-APC-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-0945M-APC-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-0945M-PE-Cy3

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy3

Catalog #1: bsm-0945M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-0945M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-0945M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-0945M-PerCP

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-0945M-PerCP-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0945M-PerCP-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bsm-0945M-RBITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bsm-1240M-BF488

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-1240M-BF350

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-1240M-BF405

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-1240M-BF555

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-1240M-BF594

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-1240M-BF647

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-1240M-BF680

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-1240M-BF750

Applications: IF(IHC-P)

Reactivity: Human, Others

Conjugate: AbBy Fluor® 750