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Image kindly submitted by Jennifer DeMuro as part of the Free Sample Program. Mouse brain lysates probed with Rabbit Anti-Leptin receptor Polyclonal Antibody (bs-0961R).
K562 cells probed with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Liver; Probed with Leptin Receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0961R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse liver lysates probed with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Rat Cerebrum lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1559R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0014R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue labeled with Anti-GLP-1 (7-36) Polyclonal Antibody, Unconjugated(bs-0038R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded fungiform papillae of rat tongue labeled with Anti-GLP-1 (7-36) Polyclonal Antibody, Unconjugated(bs-0038R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-1 Polyclonal Antibody, Unconjugated (bs-0038R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-1 Polyclonal Antibody, Unconjugated (bs-0038R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0038R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Insulin Polyclonal Antibody, Unconjugated(bs-0056R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-Insulin Polyclonal Antibody, Unconjugated(bs-0056R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse Pancreas (C57BL\/6 strain) fixed in 4% PFA for 2 hours at RT, then transferred to 30% sucrose overnight at 4\u00b0C before embedding in OCT mounting medium. Treated with TBS containing 5% goat serum and 0.3% Triton X-100, to block unspecific binding sites and permeabilize cell membranes, for 30min at RT. Antibody (bs-0056R-FITC) diluted at 1:100 in TBS containing 2% goat serum applied and incubated overnight at 4\u00b0C. Rinse sections in TBS 2x5min on a shaker, mount section with vectorshield (with DAPI), then cover them with coverslips and seal the edge of the coverslip on the slide with nail polish. This image was kindly submitted by an end-user from the National Children's Research Centre, Our Lady's Children's Hospital, Dublin, Ireland.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Insulin Polyclonal Antibody, Unconjugated (bs-0056R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0056R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0862R

Applications: WB, ELISA, IHC-P, IF(IHC-P), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human stomach tissue labeled with Anti-GLP-2 Polyclonal Antibody, Unconjugated (bs-0208R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed U2OS cells; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-2 Polyclonal Antibody, Unconjugated (bs-0208R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes and DAPI staining.

Catalog #1: bs-0208R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue labeled with Anti- Insulin Receptor alpha Polyclonal Antibody, Unconjugated(bs-0047R) 1:200 followed by conjugation to the secondary antibody and DAB staining
HL-60 cells probed with Insulin Receptor alpha Antibody, unconjugated (bs-0047R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Insulin Receptor alpha Polyclonal Antibody, Unconjugated (bs-0047R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Insulin Receptor alpha Polyclonal Antibody, Unconjugated (bs-0047R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-0047R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human pancreas carcinoma labeled with Anti-Glucagon Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Glucagon Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Glucagon Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Glucagon Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3796R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse liver labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat liver lysates L2 mouse intestine lysates probed with Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 25kD. Observed band size:25kD.
Mouse heart lysates probed with Rabbit Anti-Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0471R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-IGFBP3 Polyclonal Antibody, Unconjugated (bs-1434R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-IGFBP3 Polyclonal Antibody, Unconjugated(bs-1434R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Lane 1: HepG2 cell lysates probed with IGFBP3 Polyclonal Antibody, Unconjugated (bs-1434R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1434R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-Insulin Receptor\/CD220 Polyclonal Antibody, Unconjugated (bs-0681R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Raji cells probed with Insulin Receptor Polyclonal Antibody, unconjugated (bs-0681R) at 1:100 dilution for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)
Lane 1: Human HepG2 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human LOVO cell lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human SW480 cell lysates; Lane 6: Human K562 cell lysates probed with Insulin Receptor Polyclonal Antibody, Unconjugated (bs-0681R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0681R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti NGAL\/Lipocalin 2 Polyclonal Antibody, Unconjugated (bs-1373R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human brain labeled with Rabbit Anti-NGAL Polyclonal Antibody, Unconjugated(bs-1373R) 1:200 followed by conjugation to the PE secondary antibody and DAPI staining
Formalin-fixed and paraffin-embedded human brain labeled with Rabbit Anti-NGAL Polyclonal Antibody, Unconjugated(bs-1373R) 1:200 followed by conjugation to the PE secondary antibody

Catalog #1: bs-1373R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0406R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody (bs-0227R ) at 1:100 for 30 minutes followed by incubation with Anti-Rabbit IgG FITC conjugated secondary for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R Antibody(bs-0227R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R Polyclonal Antibody(bs-0227R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0227R

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Leptin Receptor Polyclonal Antibody(bs-0109R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0109R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Rat Brain lysates; Lane 2: Human Colon Carcinoma lysates; Probed with IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 overnight at 4˚C. Followed by secondary antibody (bs-0295G-HRP) incubation at 1:3000 for 90 min at 37˚C.
Lane 1: Human MOLT4 cell lysates; Lane 2: Human MCF-7 cell lysates probed with IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1108R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (bs-0400R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Hippocampus tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Hippocampus tissue lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Human U87MG cell lysates; Lane 9: Human HeLa cell lysates; Lane 10: Human Jurkat cell lysates probed with GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0400R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-0961R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Others

Conjugate: PE

Lane 1: mouse liver lysates Lane 2: mouse intestine lysates probed with Anti AHSG\/Fetuin A\/Alpha 2 HS Glycoprotein Polyclonal Antibody, Unconjugated (bs-2922R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 31kD. Observed band size: 31kD.
Antigen: bs-2922P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: Mouse Liver lysates probed with AHSG Polyclonal Antibody, Unconjugated (bs-2922R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2922R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti-Leptin Polyclonal Antibody (bs-0409R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:400 overnight at 4\u00b0C, DAB staining.
Leptin lysates probed with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0409R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rectal tissue labeled with Anti-Adiponectin Receptor 1 Polyclonal Antibody (bs-0610R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse bone lysates probed with Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (bs-0610R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Rat Muscle tissue lysates; Lane 5: Rat Heart tissue lysates probed with Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (bs-0610R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0610R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreatic tissue labeled with Anti-GIP Polyclonal Antibody, Unconjugated (bs-0098R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GIP Polyclonal Antibody, Unconjugated (bs-0098R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GIP) polyclonal Antibody, Unconjugated (bs-0098R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0098R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti Glucagon Receptor Polyclonal Antibody, Unconjugated (bs-3945R) followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Glucagon Receptor Polyclonal Antibody, unconjugated (bs-3945R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Glucagon Receptor Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bs-3945R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0841R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti Insulin Receptor Beta Polyclonal Antibody, Unconjugated (bs-0290R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HL-60 cells probed with Insulin Receptor Beta Antibody, unconjugated (bs-0090R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
293T lysates probed with Insulin Receptor Beta Polyclonal Antibody, Unconjugated (bs-0290R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Molt-4 lysates probed with Insulin Receptor Beta Polyclonal Antibody, Unconjugated (bs-0290R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0290R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GLP-1 Polyclonal Antibody, Unconjugated (bs-0933R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-1 Polyclonal Antibody, Unconjugated (bs-0933R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-1 Polyclonal Antibody, Unconjugated (bs-0933R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-1 Polyclonal Antibody, Unconjugated (bs-0933R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0933R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue with Anti-Resistin Polyclonal Antibody, Unconjugated (bs-0795R) at 1:400, followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Rat breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Resistin Polyclonal Antibody, Unconjugated (bs-0795R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0795R

Applications: ELISA, IHC-P

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-1822R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0961R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: FITC

Lane 1: mouse embryo lysates probed with Rabbit Anti-GAD65 + GAD67 Polyclonal Antibody, Unconjugated (bs-13263R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse brain tissue probed with Anti-GAD65 + GAD67 Polyclonal Antibody (bs-13263R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GAD65 + GAD67) polyclonal Antibody, Unconjugated (bs-13263R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-13263R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-6333R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-1 (1G9) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-1 (1G9) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-1 (1G9) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-0933M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human rectal tissue labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Heart lysates; Lane 2: Mouse Skeletal muscle lysates; Lane 3: Rat Heart lysates; Lane 4: Rat Skeletal muscle lysates; Lane 5: Human 293T cell lysates probed with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0611R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0961R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Lane 1: Human Raji lysates; Lane 2: Mouse spleen lysates probed with Rabbit Anti-ZNT8 Polyclonal Antibody, Unconjugated (bs-15382R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with ZNT8 Polyclonal Antibody (bs-15382R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-15382R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-0108R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0056R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bs-15407R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Mouse liver lysates probed with RELMa\/RELM alpha Polyclonal Antibody, Unconjugated (bs-1884R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Mouse liver lysates probed with RELMa\/RELM alpha Polyclonal Antibody, Unconjugated (bs-1884R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1884R

Applications: WB, ELISA, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3796R-A488

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-7800R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

L1 rat brain, L2 rat liver lysates probed (bs-0272R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 54kDa.
Formalin-fixed and paraffin embedded rat lung labeled with Anti-PBEF1 Polyclonal Antibody, Unconjugated (bs-0272R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human kidney labeled with Anti-PBEF1 Polyclonal Antibody, Unconjugated (bs-0272R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse heart lysates probed with PBEF1 Polyclonal Antibody, Unconjugated (bs-0272R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0272R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human pancreas labeled with Anti-IGF II\/IGF2 Polyclonal Antibody, Unconjugated (bs-0015R), followed by conjugation to the secondary antibody and DAB staining
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF 2 Polyclonal Antibody(bs-0015R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0015R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0227R-A647

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-0260R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0961R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-0961R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 647