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Hepatology

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Catalog #1: bsm-43141M-BF750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-43141M-APC

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-43141M-APC-Cy5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5

Catalog #1: bsm-43141M-APC-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-43141M-APC-Cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-43141M-PE-Cy3

Applications: WB

Reactivity: Human

Conjugate: PE-Cy3

Catalog #1: bsm-43141M-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-43141M-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-43141M-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-43141M-PerCP

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-43141M-PerCP-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43141M-PerCP-Cy7

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-43141M-RBITC

Applications: WB

Reactivity: Human

Conjugate: RBITC

Antigen: bs-0719P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CEA Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CEAM5 Antibody(bs-0719R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEAM5 Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0719R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated