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Lipid Peroxidation and Oxidative Stress

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Paraformaldehyde-fixed, paraffin embedded Human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P53(Mono Methyl Lys370) Monoclonal Antibody, Unconjugated (bsm-33209M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P53(Mono Methyl Lys370) Monoclonal Antibody, Unconjugated (bsm-33209M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P53(Mono Methyl Lys370) Monoclonal Antibody, Unconjugated (bsm-33209M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Urinary bladder lysates; Lane 2: Mouse Skin lysates probed with P53 Monoclonal Antibody, Unconjugated (bsm-33209M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33209M

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-10841M-Biotin

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33007M-HRP

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP

Catalog #1: bsm-33007M-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

GST-Tagged fusion protein Overexpression E.coli Lysates probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST-Tagged fusion protein Overexpression E.coli Lysates probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:2000~1:10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST-Tagged fusion protein probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:60000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33007M

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bsm-33209M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33209M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-33209M-Cy3

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-33209M-Cy5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-33209M-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-33209M-Cy7

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-33209M-FITC

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33232M

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33232M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33232M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33232M-Cy5

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33232M-Cy3

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33232M-Cy5.5

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33232M-Cy7

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33232M-FITC

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33234M

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33234M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33234M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33234M-Cy3

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33234M-Cy5

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33234M-Cy5.5

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33234M-Cy7

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33234M-FITC

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33278M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33278M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33282M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33282M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33282M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33278M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-AKT1(S124) Monoclonal Antibody, Unconjugated (bsm-54623R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-AKT1(S124) Monoclonal Antibody, Unconjugated (bsm-54623R) at 1:200 for 30 minutes at room temperature, DAB staining.
PC-3M cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-AKT1(S124)) polyclonal Antibody, Unconjugated (bsm-54623R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-AKT1(S124)) polyclonal Antibody, Unconjugated (bsm-54623R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Catalog #1: bsm-54623R

Applications: WB, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54652R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33117M-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33117M-Cy3

Applications: WB, IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-33117M-FITC

Applications: WB, IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-33117M-Cy7

Applications: WB, IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33117M-Cy5

Applications: WB, IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Paraformaldehyde-fixed, paraffin embedded Human prostate cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT2 Monoclonal Antibody, Unconjugated (bsm-51473M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse brain tissue lysates probed with AKT2 Monoclonal Antibody, Unconjugated (bsm-51473M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: Hela cell lysates; Lane 3: Mouse brain tissue lysates; Lane 4: C6 cell lysates probed with AKT2 Monoclonal Antibody, Unconjugated (bsm-51473M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51473M

Applications: WB, IHC-P, IF

Reactivity:

Conjugate: Unconjugated

Lane 1: HL-60 cell lysates probed with AMPK alpha 2 Monoclonal Antibody, Unconjugated (bsm-51482M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: 293 cell lysates probed with AMPK alpha 2 Monoclonal Antibody, Unconjugated (bsm-51482M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51482M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51706M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Lane 1: U-87MG cell lysates probed with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-51741M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human LOVO cell lysates probed with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-51741M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51741M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51742M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:3000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1:SH-SY5Y, 2: C2C12, 3: HepG2, 4: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60\/10\/60 s\r\nPredicted MW: 60 kDa\r\nObserved MW: 60 kDa\r\n
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-60450M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Tissue: Human placenta Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60581R
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: Jurkat, 2: PC-3, 3: MOLT-4, 4: K562\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 23 kDa\r\nObserved MW: 23 kDa\r\n
Cell line: K562\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60581R
Cell line: HepG2\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 90% Methanol\r\nPrimary Ab dilution: 1:100\r\nSecondary Ab: Goat anti Rabbit IgG\r\nUnlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).\r\nIsotype control: Rabbit monoclonal IgG (Blue line).\r\nComment: Line red is the positive signal for bsm-60581R

Catalog #1: bsm-60581R

Applications: WB, IHC-P, IF, ICC

Reactivity: Human

Conjugate: Unconjugated