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Muscular Dystrophies and Neuromuscular Diseases

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HUVEC cell lysates probed with Caveolin-2 (25B3) Polyclonal Antibody, Unconjugated (bsm-52382R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
IF(ICC) staining with Caveolin-2 (25B3) Monoclonal Antibody (bsm-52382R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with Caveolin-2 (25B3) Monoclonal Antibody (bsm-52382R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52382R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Human heart lysates probed with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human heart tissue incubated with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52818R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of MCF7 cells with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: K562 lysates; Lane 2: HepG2 lysates; Line 3: A431 lysates; Probed with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52792R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse brain; Lane 2: SH-SY-5Y cell; Probed with TrkA (4G1 ) Monoclonal Antibody (bsm-52424R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of SH-SY-5Y cells with TrkA (4G1) Monoclonal Antibody (bsm-52424R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with TrkA (4G1) Monoclonal Antibody (bsm-52424R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with TrkA (4G1) Monoclonal Antibody (bsm-52424R) at 1:100 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52424R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1:Human skeletal muscle lysates; Lane 2: Mouse heart lysates probed with Desmin (5A5) Monoclonal Antibody, Unconjugated (bsm-52363R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
IF(ICC) staining with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100 in C2C12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse bladder tissue incubated with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52363R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54658R

Applications: WB, FCM, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated