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Muscular Dystrophies and Neuromuscular Diseases

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Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti Integrin alpha 4\/CD49d\/Integrin \u03b14 Polyclonal Antibody, Unconjugated (bs-0641R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with Integrin alpha 4\/CD49d Antibody, Unconjugated (bs-0641R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
U-937 (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4\/CD49dAntibody( bs-0641R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4 Polyclonal Antibody(bs-0641R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0641R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Integrin beta 1\/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Integrin beta 1\/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human A431 cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Rat Ear lysates; Lane 4: Mouse Ear lysates; Lane 5: Mouse Urinary bladder lysates probed with Integrin beta 1 Polyclonal Antibody, Unconjugated (bs-0486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Integrin beta 1) polyclonal Antibody, Unconjugated (bs-0486R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0486R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Rabbit Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Laminin Polyclonal Antibody, Unconjugated (bs-0821R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Laminin Polyclonal Antibody, Unconjugated (bs-0821R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Laminin Polyclonal Antibody, Unconjugated (bs-0821R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-0821R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat adrendal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tRabbit Anti-Integrin alpha L\/CD11a Polyclonal Antibody, Unconjugated (bs-1804R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Human U937 cells probed with Integrin alpha L\/CD11a Antibody, Unconjugated (bs-1804R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1804R

Applications: FCM, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rabbit heart labeled with Anti CTn1\/Troponin I\/TNNC1 Polyclonal Antibody,Unconjugated (bs-0799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse heart tissue with Anti-CTn1\/Troponin I\/TNNC1 Polyclonal Antibody, Unconjugated (bs-0799R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-0799R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0486R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: FITC

Catalog #1: bs-0641R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: Cy7

Catalog #1: bs-0799R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: FITC

Catalog #1: bs-0799R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: HRP

Catalog #1: bs-0641R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rabbit

Conjugate: Biotin

Catalog #1: bs-0799R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bs-0486R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy3

Catalog #1: bs-0486R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy5

Catalog #1: bs-0486R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy7

Catalog #1: bs-0486R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Biotin

Catalog #1: bs-0486R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: HRP

Catalog #1: bs-0641R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: Cy3

Catalog #1: bs-0641R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: Cy5

Catalog #1: bs-0641R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: FITC

Catalog #1: bs-0641R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: PE

Catalog #1: bs-0641R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rabbit

Conjugate: HRP

Catalog #1: bs-0799R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bs-0799R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bs-0799R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bs-0486R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PE

Catalog #1: bs-0486R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy5.5

Catalog #1: bs-0641R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0799R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0486R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0486R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0486R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0486R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0486R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0486R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0486R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0486R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0486R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: APC

Catalog #1: bs-0486R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: APC-Cy5

Catalog #1: bs-0486R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: APC-Cy5.5

Catalog #1: bs-0486R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: APC-Cy7

Catalog #1: bs-0486R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PE-Cy3

Catalog #1: bs-0486R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PE-Cy5

Catalog #1: bs-0486R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PE-Cy5.5

Catalog #1: bs-0486R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PE-Cy7

Catalog #1: bs-0486R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PerCP

Catalog #1: bs-0486R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0486R-PerCP-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PerCP-Cy7

Catalog #1: bs-0486R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: RBITC