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Musculoskeletal Biology

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Catalog #1: bs-20782R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Sheep

Conjugate: HRP

Lane 1: Hela Cell lysates; Lane 2: Molt-4 Cell lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-23426R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Raji Cell lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-23426R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Jurkat Cell lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-23426R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-23426R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-23426R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0078R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: Biotin

Catalog #1: bs-0078R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: HRP

THP-1 cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF alpha Polyclonal Antibody(bs-41192R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raw264.7 cells were treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation).The cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at at room temperature and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF alpha Polyclonal Antibody(bs-41192R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-41192R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-41192R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-41192R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-2459R-Biotin

Applications: WB, ELISA

Reactivity:

Conjugate: Biotin

Catalog #1: bs-2459R-HRP

Applications: WB, ELISA

Reactivity:

Conjugate: HRP

Catalog #1: bs-20781R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-20781R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-20781R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0647R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: HRP

Catalog #1: bs-0766R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

Catalog #1: bs-0766R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: HRP

Catalog #1: bs-10699R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10699R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-23447R-HRP

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-23447R-Biotin

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-0648R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0648R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP