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Musculoskeletal Biology

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Human thymus lysate probed with CD4 (65H6) Monoclonal Antibody (bsm-52469R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CD4 (65H6) Monoclonal Antibody (bsm-52469R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD4 (65H6) Monoclonal Antibody (bsm-52469R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD4 (65H6) Monoclonal Antibody (bsm-52469R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52469R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat cell lysates; Lane 2: Human thymus lysates probed with CD3 epsilon (7A4 ) Monoclonal Antibody (bs-7537R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Flow cytometric analysis of Hela cells with CD3 epsilon (7A4) Monoclonal Antibody (bsm-52386R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD3 epsilon (7A4) Monoclonal Antibody (bsm-52386R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD3 epsilon (7A4) Monoclonal Antibody (bsm-52386R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52386R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Raji cell lysates probed with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52346R

Applications: WB, IHC-P, IHC

Reactivity: Human, Monkey

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: Lovo cell lysates probed with CD8 alpha (72B1) Monoclonal Antibody (bsm-52364R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD8 alpha (72B1) Monoclonal Antibody (bsm-52364R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52364R

Applications: WB, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of Raji cells with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Jurkat cell lysate probed with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:300 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:300 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52744R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of Jurkat cells with HLA-DR (32G3) Monoclonal Antibody (bsm-52535R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Daudi cell lysate probed with HLA-DR (32G3) Monoclonal Antibody (bsm-52535R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with HLA-DR (32G3) Monoclonal Antibody (bsm-52535R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with HLA-DR (32G3) Monoclonal Antibody (bsm-52535R) at 1:100 in B16-F1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52535R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated