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Parkinson's Disease

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Lane 1: HepG2 cells lysates; Lane 2: 293T brain cells lysates; Probed with GBA (4C2) Monoclonal Antibody (bsm-52907R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with GBA (4C2) Monoclonal Antibody (bsm-52907R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with GBA (4C2) Monoclonal Antibody (bsm-52907R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with GBA (4C2) Monoclonal Antibody (bsm-52907R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52907R

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52014M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Fetal Brain; Probed with Alpha Synuclein (3H12) Monoclonal Antibody (bsm-54277R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Alpha Synuclein (3H12) Monoclonal Antibody (bsm-54277R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Alpha Synuclein (3H12) Monoclonal Antibody (bsm-54277R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of SH-SY5Y cells with Alpha Synuclein (3H12) Monoclonal Antibody (bsm-54277R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54277R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

IF(ICC) staining with Phospho-alpha Synuclein (Ser129) (3G7) Monoclonal Antibody (bsm-54276R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-alpha Synuclein (Ser129) (3G7) Monoclonal Antibody (bsm-54276R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-alpha Synuclein (Ser129) (3G7) Monoclonal Antibody (bsm-54276R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Phospho-alpha Synuclein (Ser129) (3G7) Monoclonal Antibody (bsm-54276R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54276R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of PC12 cells with nNOS (14G6) Monoclonal Antibody (bsm-52474R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Mouse heart lysate probed with nNOS (14G6) Monoclonal Antibody (bsm-52474R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with nNOS (14G6) Monoclonal Antibody (bsm-52474R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with nNOS (14G6) Monoclonal Antibody (bsm-52474R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52474R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with PARK7\/DJ1 (28B2) Monoclonal Antibody (bsm-52604R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela Cells; Lane 2: Jurkat Cells; Lane 3: NIH\/3T3 Cells; Probed with PARK7\/DJ1 (28B2) Monoclonal Antibody (bsm-52604R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PARK7\/DJ1 (28B2) Monoclonal Antibody (bsm-52604R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PARK7\/DJ1 (28B2) Monoclonal Antibody (bsm-52604R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52604R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-Alpha synuclein(Ser129) Monoclonal Antibody, Unconjugated (bsm-41203M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-Alpha synuclein(Ser129) Monoclonal Antibody, Unconjugated (bsm-41203M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-Alpha synuclein(Ser129) Monoclonal Antibody, Unconjugated (bsm-41203M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-Alpha synuclein(Ser129) Monoclonal Antibody, Unconjugated (bsm-41203M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-41203M

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41203M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-41203M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-41203M-Cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-41203M-Cy3

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-41203M-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-41203M-Cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-41203M-FITC

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-41203M-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-51545M

Applications: WB, IHC-P

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51696M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (bsm-41217M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (bsm-41217M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (bsm-41217M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (bsm-41217M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-41217M

Applications: IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41217M-Biotin

Applications: IHC-P

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-41217M-HRP

Applications: IHC-P

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-42005M-RBITC

Applications: WB

Reactivity: Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-42005M-PerCP-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-42005M-PerCP-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-42005M-PerCP

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-42005M-PE-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-42005M-PE-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-42005M-PE-Cy5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-42005M-PE-Cy3

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bsm-42005M-APC-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-42005M-APC-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-42005M-APC-Cy5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bsm-42005M-APC

Applications: WB

Reactivity: Mouse, Rat

Conjugate: APC

Catalog #1: bsm-42005M-BF750

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-42005M-BF680

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-42005M-BF647

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-42005M-BF594

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-42005M-BF555

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-42005M-BF488

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-42005M-BF405

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-42005M-BF350

Applications: WB

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-42005M-HRP

Applications: WB

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-42005M-Biotin

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-42005M-PE

Applications: WB

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bsm-42005M-FITC

Applications: WB

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-42005M-Cy7

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-42005M-Cy5.5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-42005M-Cy5

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-42005M-Cy3

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Cy3