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Primary Antibodies

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Catalog #1: bsm-33377M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Lane 1: LOVO lysates; Lane 2: MCF-7 lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-33411M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4\u00b0C, DAB staining.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, Unconjugated (bs-33411M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33411M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33411M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33411M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-4533M

Applications: IHC-P, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-4533M-Cy5

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy5

Catalog #1: bsm-4533M-Cy3

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy3

Catalog #1: bsm-4533M-Cy7

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy7

Catalog #1: bsm-4533M-FITC

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: FITC

Catalog #1: bsm-4533M-HRP

Applications: IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bsm-4541M

Applications: ELISA

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-4533M-Biotin

Applications: IHC-P

Reactivity: Others

Conjugate: Biotin

Catalog #1: bsm-4792M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-4792M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-4792M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-4792M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-4792M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-4792M-Biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-4792M-HRP

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

HT29 Cell lysates probed with CEA (5F2) Monoclonal Antibody, unconjugated (bsm-4793M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEA (5F2) Monoclonal Antibody, Unconjugated (bsm-4793M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-4793M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-4793M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-4793M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-4793M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-4793M-Biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-4793M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-4793M-HRP

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Lane 1: HepG2 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: NCI-H460 Cell lysates; probed with ALDH1A1 (2C2) Monoclonal Antibody, unconjugated (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NCI-H460 Cells; 15 ug of protein lysate loaded; Probed with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:25 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin (red). Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X100\/PBS.

Catalog #1: bsm-51032M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51056M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: CEM Cell lysates; Lane 3: HepG2 Cell lysates; Lane 4: Jurkat Cell lysates; Lane 5: HeLa Cell lysates; Lane 6: Raji Cell lysates; probed with H2AFX (8C5) Monoclonal Antibody, unconjugated (bsm-51222M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Thymus tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human prostate tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody, Unconjugated (bsm-51222M) at 1:50 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-51222M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51225M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51238M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51240M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51300M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HL-60; Lane 2: K562 cell lysate at 20 \u00b5g per lane, probed with bsm-51424M ITGB2 (534CT6.2.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ITGB2 (534CT6.2.1) Monoclonal Antibody (bsm-51424M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51424M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52001M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: F9; Lane 2: NCCIT; Lane 3: hES lysates probed with Oct 4 (2D5) Monoclonal Antibody (bsm-52002R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
N2A cells were stained with Oct 4 (2D5) Monoclonal Antibody (bsm-52002R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Western blot analysis of Oct4 on F9 cell lysates with Rabbit anti-Oct4 antibody (bsm-52002R) at 1\/1,000 dilution.\r\n\r\nLysates\/proteins at 10 \u788cg\/Lane.\r\n\r\nPredicted band size: 39 kDa\r\nObserved band size: 45 kDa\r\n\r\nExposure time: 2 minutes;\r\n\r\n12% SDS-PAGE gel.\r\n\r\nProteins were transferred to a PVDF membrane and blocked with 5% NFDM\/TBST for 1 hour at room temperature. The primary antibody (bsm-52002R) at 1\/1,000 dilution was used in 5% NFDM\/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.\r\n
Western blot analysis of Oct4 on different lysates with Rabbit anti-Oct4 antibody (bsm-52002R) at 1\/500 dilution.\r\n\r\nLane 1: HES cell lysate\r\nLane 2: NCCIT cell lysate\r\n\r\nLysates\/proteins at 10 \u00b5g\/Lane.\r\n\r\nPredicted band size: 39 kDa\r\nObserved band size: 45 kDa\r\n\r\nExposure time: 1 minute;\r\n\r\n10% SDS-PAGE gel.\r\n\r\nProteins were transferred to a PVDF membrane and blocked with 5% NFDM\/TBST for 1 hour at room temperature. The primary antibody (bsm-52002R) at 1\/500 dilution was used in 5% NFDM\/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.\r\n

Catalog #1: bsm-52002R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Western blot analysis of 4E-BP1 expression in non-transfected (A) and mouse 4E-BP1 transfected (B) 293T whole cell lysates.
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells using 4E-BP1 (3G1) Monoclonal Antibody (bsm-52007R)

Catalog #1: bsm-52007R

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52008M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

MCF-7 lysates probed with AKT1 (11F2) Monoclonal Antibody (bsm-52010R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Thymus lysates; Lane 3: Mouse Heart lysates; Lane 4: Mouse Lung lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Cerebellum lysates; Lane 7: Rat Thymus lysates; Lane 8: Rat Heart lysates; Lane 9: Human MCF-7 cell lysates; Lane 10: Human Hela cell lysates; Lane 11: Human U937 cell lysates; Lane 12: Human A549 cell lysates probed with AKT1 (11F2) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1 (11F2) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1 (11F2) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52010R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52019R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7 lysates probed with Bad (5D4) Monoclonal Antibody (bsm-52020R) at 1:500.
Paraformaldehyde-fixed, paraffin embedded; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Bad (5D4) Monoclonal Antibody,(bsm-52020R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bad (5D4) Monoclonal Antibody, Unconjugated (bsm-52020R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bad (5D4) Monoclonal Antibody, Unconjugated (bsm-52020R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52020R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Bcl-XL (12C5) Monoclonal Antibody (bsm-52024R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Bcl-XL (12C5) Monoclonal Antibody (bsm-52024R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human A549 cell lysates probed with Bcl-XL Monoclonal Antibody, Unconjugated (bsm-5202M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52024R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52037M

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293; Lane 2: F9; Lane 3: PC12; Lane 4: Hela lysates probed with Cyclin A2 (2E9) Monoclonal Antibody (bsm-52043M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human uterus \r\ntissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin A2 (2E9) Monoclonal Antibody (bsm-52043M) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52043M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: PC12 Cell lysates; Lane 3: SH-SY5Y Cell lysates;probed with Cyclin D1 (2H4) Monoclonal Antibody (bsm-52046R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin D1 (2H4) Monoclonal Antibody (bsm-52046R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin D1 (2H4) Monoclonal Antibody, Unconjugated (bsm-52046R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human U251 cell lysates probed with Cyclin D1 Monoclonal Antibody, Unconjugated (bsm-52046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52046R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293T Cell lysates; Lane 2: HeLa cell lysates; probed with Cytokeratin 1 (1C2) Monoclonal Antibody (bsm-52051R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 1 (1C2) Monoclonal Antibody (bsm-52051R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52051R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HeLa lysates; probed with Cytokeratin 10 (1D8) Monoclonal Antibody (bsm-52052R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 10 (1D8) Monoclonal Antibody (bsm-52052R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 10 (1D8) Monoclonal Antibody (bsm-52052R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Skin lysates; Lane 2: Rat Skin lysates; Lane 3: Hela cell lysates probed with Cytokeratin 10 Monoclonal Antibody, Unconjugated (bsm-52052R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52052R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: Human skin lysates; probed with Cytokeratin 14 (2F11) Monoclonal Antibody (bsm-52054R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 14 (2F11) Monoclonal Antibody (bsm-52054R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 14 (2F11) Monoclonal Antibody (bsm-52054R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 14 (2F11) Monoclonal Antibody, Unconjugated (bsm-52054R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52054R

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated