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Primary Antibodies

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Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Placenta lysates\uff1bLane 2: Mouse Lung lysates probed with AKT1\/2\/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (bs-0115R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0115R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Unconjugated

Catalog #1: bs-0115R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Biotin

Catalog #1: bs-0115R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy3

Catalog #1: bs-0115R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy5

Catalog #1: bs-0115R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: FITC

Catalog #1: bs-0115R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy7

Catalog #1: bs-0876R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: HRP

Catalog #1: bs-0876R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Biotin

Formalin-fixed and paraffin embedded human endometrium tissue labeled with Anti-phospho-AKT\/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse tumor with labeled Anti-phospho-AKT\/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-AKT(Ser473) Polyclonal Antibody, Unconjugated (bs-0876) at 1:300 overnight at 4˚C. Incubate with HRP conjugated Goat-Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000 for 90min at 37˚C.
U937 cell lysates probed with Anti-AKT1\/2\/3 (Ser472\/Ser473\/Ser474) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0876R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Unconjugated

Catalog #1: bs-0876R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy3

Catalog #1: bs-0876R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy5

Catalog #1: bs-0876R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: FITC

Catalog #1: bs-0876R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy7

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit pancreas tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat lung lysate 30ug, probed (bs-0081R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

Catalog #1: bs-0081R

Applications: WB, ELISA, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Catalog #1: bs-0081R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: HRP

Catalog #1: bs-0081R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Biotin

Catalog #1: bs-0081R-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Cy7

Catalog #1: bs-0081R-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Cy5

Catalog #1: bs-0081R-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Cy3

Catalog #1: bs-0081R-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: FITC

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Spleen lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.
Hela lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1287R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: Unconjugated

Catalog #1: bs-1287R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: Cy3

Catalog #1: bs-1287R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: Cy5

Catalog #1: bs-1287R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: Biotin

Catalog #1: bs-1287R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: HRP

Catalog #1: bs-1287R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: Cy7

Catalog #1: bs-1287R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: FITC

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CDH2\/N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDH2\/N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse breast cancer lysates probed with Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-1172R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: Unconjugated

Catalog #1: bs-1172R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: HRP

Catalog #1: bs-1172R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: Cy3

Catalog #1: bs-1172R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: Biotin

Catalog #1: bs-1172R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: Cy5

Catalog #1: bs-1172R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: Cy7

Catalog #1: bs-1172R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: FITC

Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Kamlesh Gupta as part of the free sample program. Caco-2 cell lysate and mouse kidney lysate probed with ZO-1 primary antibody (bs-1329R) at 1:500 overnight at 4˚C. Followed by incubation with anti-rabbit HRP-conjugated secondary antibody at 1:5000 dilution for 1h at 25˚C. Predicted and observed band size: ~200kDa.
Image provided by the Independent Validation Program, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.
293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1329R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-1329R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy5

Catalog #1: bs-1329R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy3

Catalog #1: bs-1329R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy7

Catalog #1: bs-1329R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PE

Catalog #1: bs-1329R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: FITC

Catalog #1: bs-1329R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: HRP

Catalog #1: bs-1329R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Biotin

Formalin-fixed and rat pancreas tissue labeled with Anti-FN\/Fibronectin 1 Polyclonal Antibody, Unconjugated (bs-0666R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)
Image kindly submitted by Edita Aksamitiene as part of the free sample program. Rat skin lysates probed with Rabbit Anti-Fibronectin Polyclonal Antibody (bs-0666R) at 1:1000.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Fibronectin Polyclonal Antibody (bs-0666R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded rat intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated (bs-0666R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-0666R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Unconjugated

Catalog #1: bs-0666R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Biotin

Catalog #1: bs-0666R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: HRP

Catalog #1: bs-0666R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Cy3

Catalog #1: bs-0666R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Cy5

Catalog #1: bs-0666R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Cy7