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Primary Antibodies

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Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated(bs-0279R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Cells labeled with Anti-VEGF Polyclonal Antibody (bs-0279R).
Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 23,46kDa
HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated.\\n

Catalog #1: bs-0279R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Unconjugated

Catalog #1: bs-0279R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Biotin

Catalog #1: bs-0279R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: HRP

Catalog #1: bs-0279R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Cy5

Catalog #1: bs-0279R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: FITC

Catalog #1: bs-0279R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Cy7

Catalog #1: bs-0279R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Cy3

Catalog #1: bs-0279R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: PE

Catalog #1: bs-0292R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat, Bovine, Goat, Others, Cow

Conjugate: Biotin

Catalog #1: bs-0292R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat, Bovine, Goat, Others, Cow

Conjugate: HRP

Catalog #1: bs-0292R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat, Others, Cow

Conjugate: Cy5

Antigen: bs-0292P, BSA, 1ug\/100ul \\nPrimary: bs-0292R, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm. \\n
Lane 1: Human Serum lysates; Lane 2: Mouse Serum lysates; Lane 3: Rat Serum lysates; Lane 4: Goat Serum lysates; Lane 5: Cow Serum lysates probed with BSA Polyclonal Antibody, Unconjugated (bs-0292R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0292R

Applications: WB, ELISA, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat, Others, Cow

Conjugate: Unconjugated

Catalog #1: bs-0292R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat, Others, Cow

Conjugate: Cy3

Catalog #1: bs-0292R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat, Others, Cow

Conjugate: FITC

Catalog #1: bs-0292R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat, Others, Cow

Conjugate: Cy7

Catalog #1: bs-0351R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: Cy3

Catalog #1: bs-0351R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: HRP

Catalog #1: bs-0351R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: Biotin

Catalog #1: bs-0351R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: Cy5

Human HT-29 lysates probed with Rabbit Anti-Glut2 Polyclonal Antibody, Unconjugated (bs-0351R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Hep G2 cells probed with GLUT2 Polyclonal Antibody, unconjugated (bs-0351R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Lane 1: Mouse embyo lysates probed with GLUT2 Polyclonal Antibody, Unconjugated (bs-0351R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0351R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: Unconjugated

Catalog #1: bs-0351R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: FITC

Catalog #1: bs-0351R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: Cy7

Catalog #1: bs-0351R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Goat, Others

Conjugate: PE

Catalog #1: bs-0466R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine, Sheep, Goat, Cow

Conjugate: Unconjugated

Lane 1: mouse embryo lysates Lane 2: mouse lung lysates probed with Anti SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 32kD. Observed band size: 32kD.
NCCIT cells probed with Rabbit Anti-SOX2 Polyclonal Antibody (bs-0523R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Paraformaldehyde-fixed, paraffin embedded Mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:400 overnight at 4\u00b0C, DAB staining.
U251 cells (black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX2 Antibody(bs-0523R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0523R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-0523R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy3

Catalog #1: bs-0523R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Biotin

Catalog #1: bs-0523R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat

Conjugate: HRP

Catalog #1: bs-0523R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy5

Catalog #1: bs-0523R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Cy7

Catalog #1: bs-0523R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Goat

Conjugate: PE

Catalog #1: bs-0523R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: FITC

Ewe's milk probed with Anti Beta-lactoglobulin Polyclonal Antibody, Unconjugated (bs-0814R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 19kD. Observed band size: 19kD
Paraformaldehyde-fixed, paraffin embedded Sheep breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with beta lactoglobulin Polyclonal Antibody, Unconjugated (bs-0814R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Sheep breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta lactoglobulin) Polyclonal Antibody, Unconjugated (bs-0814R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-0814R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Sheep, Goat, Cow

Conjugate: Unconjugated

Catalog #1: bs-0814R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Sheep, Goat, Cow

Conjugate: HRP

Catalog #1: bs-0814R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Sheep, Goat, Cow

Conjugate: Biotin

Catalog #1: bs-0814R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Sheep, Goat, Cow

Conjugate: Cy3

Catalog #1: bs-0814R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Sheep, Goat, Cow

Conjugate: Cy5

Catalog #1: bs-0814R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Sheep, Goat, Cow

Conjugate: Cy7

Catalog #1: bs-0814R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Sheep, Goat, Cow

Conjugate: FITC

Catalog #1: bs-0923R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-0923R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat, Goat

Conjugate: Biotin

Catalog #1: bs-0923R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat, Goat

Conjugate: HRP

Catalog #1: bs-0923R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Goat

Conjugate: Cy3

Catalog #1: bs-0923R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Goat

Conjugate: Cy5

Catalog #1: bs-0923R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Goat

Conjugate: FITC

Catalog #1: bs-0923R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Goat

Conjugate: Cy7

HepG2 lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bs-0945R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human Serum; Lane 2: Mouse Serum; Lane 3: Rat Serum; Lane 4: Goat Serum; Lane 5: Rat Liver lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bs-0945R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Albumin) polyclonal Antibody, Unconjugated (bs-0945R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Serum; Lane 2: Rat Serum; Lane 3: Human Serum; Lane 4: Goat Serum probed with Human Serum albumin Polyclonal Antibody, Unconjugated (bs-0945R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0945R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-0945R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Goat

Conjugate: HRP