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Paraformaldehyde-fixed, paraffin embedded Human Skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CD44 (Hermes-3) Monoclonal Antibody (bsm-51065M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung adenocarcinoma section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CD44 (Hermes-3) Monoclonal Antibody (bsm-51065M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded SAMPLE tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CD44 (Hermes-3) Monoclonal Antibody (bsm-51065M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD44 (H3) Monoclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-51065M

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

A431 cell lysate probed with ACTA2 (3F9) Monoclonal Antibody (bsm-33187M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:400 overnight at 4\u00b0C, followed by incubation with a secondary antibody, DAB and counter-staining.
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Small intestine lysates; Lane 6: Mouse NIH\/3T3 cell lysates; Lane 7: Rat Stomach lysates; Lane 8: Rat Uterus lysates; Lane 9: Human A549 cell lysates; Lane 10: Human Hela cell lysates; Lane 11: Human A431 cell lysates; Lane 12: Human HepG2 cell lysates probed with ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33187M

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

KLF4 recombinant protein (20ug) probed with bsm-51264M KLF4 (5A1) Monoclonal Antibody at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: KLF4 protein; probed with KLF4 (5A1) Monoclonal Antibody, unconjugated (bsm-51264M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51264M

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33187M-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33187M-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33187M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

NCCIT cell lysate probed with bsm-51142M SOX2 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: SOX2 Protein Isolate; probed with SOX2 (7C4) Monoclonal Antibody, unconjugated (bsm-51142M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:50 (37\u2103 for 1hr) in A549 cells (green). Cells were counterstained with Alexa Fluor\u00ae 555 (red) conjugated Phalloidin. Cells were fixed in 4% paraformaldehyde for 20min and permeabilized with 0.1% Triton X100\/PBS for 10min.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-51142M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51200M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51276M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51091M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33187M-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33187M-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33187M-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33187M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-52001M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33187M-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Myoglobin (3A7) Monoclonal Antibody, Unconjugated (bsm-41107M) at 1:500 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Myoglobin) Monoclonal Antibody, Unconjugated (bsm-41107M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Myoglobin) Monoclonal Antibody, Unconjugated (bsm-41107M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Lane 1: Rat Muscle tissue lysates probed with Myoglobin Monoclonal Antibody, Unconjugated (bsm-41107M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-41107M

Applications: IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41107M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-41107M-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-41107M-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-41107M-Cy5.5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-41107M-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-41107M-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-41107M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-33187M-BF350

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-33187M-BF405

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-33187M-BF488

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-33187M-BF555

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-33187M-BF594

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-33187M-BF647

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-33187M-BF680

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-33187M-BF750

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-33187M-APC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33187M-APC-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bsm-33187M-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33187M-APC-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33187M-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bsm-33187M-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33187M-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33187M-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33187M-PerCP

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33187M-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33187M-PerCP-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33187M-RBITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-41107M-BF350

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-41107M-BF405

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-41107M-BF488

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-41107M-BF555

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 555