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Tumor Immunology

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Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 mouse lung lysates L2 protein provided by the end user lysates probed with Anti IL-6 Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 23kD. Observed band size:23kD.

Catalog #1: bs-0782R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon tissue labeled Anti-IL-10 Polyclonal Antibody, Unconjugated(bs-0698R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse spleen lysates probed with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody\r\nincubation at 1:20000 for 60 min at 37˚C.\r\n
Lane 1: Human THP-1 cell lysates; Lane 2: Human THP-1-LPS\r\ncell lysates probed with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0698R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with (bs-0812R) Rabbit Anti-IL-1 Beta\/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.
Image was kindly submitted by Dr.Rachel Reith, NIH. Paraffin-embedded mouse brain stained with Rabbit Anti-IL-1 Beta Polyclonal Antibody,1:100 overnight at 4C.\\n
Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin-embedded Human Cervical Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C. Followed by incubation with the secondary antibody, DAB and counterstaining.

Catalog #1: bs-0812R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), dot-blot

Reactivity: Human, Mouse, Rat, Bovine, Pig, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat renal tissue labeled with Anti-TGF-beta-1 Polyclonal Antibody, Unconjugated(bs-0086R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Isolated Transforming growth factor beta-1; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Embryo; Lane 2: Rat Cerebrum; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:1000 dilution, overnight at 4˚C. Followed by secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0086R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated(bs-0279R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Cells labeled with Anti-VEGF Polyclonal Antibody (bs-0279R).
Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 23,46kDa
HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated.\\n

Catalog #1: bs-0279R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse kidney cells labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0766R) 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded Lymphocytes labeled with (bs-0766R) Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated followed by incubation with a conjugated secondary antibody.
Formalin-fixed and paraffin embedded Lymphocytes labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) followed by incubation with a conjugated secondary antibody and DAPI staining.

Catalog #1: bs-0766R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (bs-0465R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated (bs-0465R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0465R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat carotid artery Anti-MMP-2 Polyclonal Antibody (bs-0412R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated bs-0412R at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated (bs-0412R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mmp2 Polyclonal Antibody, Unconjugated (bs-0412R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0412R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Placenta lysates\uff1bLane 2: Mouse Lung lysates probed with AKT1\/2\/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (bs-0115R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0115R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti p38MAPK\/MAPK14\/p38Alpha Polyclonal Antibody, Unconjugated (bs-0637R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Independently Validated Antibody, image provided by Science Exchange, badge number 029806: L1 HeLa cell lysate, L2 C6\/36 cell lysates probed with Anti-P38 MAPK Polyclonal Antibody, Unconjugated (bs-0637R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:100000 for 90 min at 37˚C. Predicted band 41kDa. A strong band was observed in the positive control sample at the correct molecular weight.
MCF-7 cells were stained with p38 MAPK Polyclonal Antibody, Unconjugated(bs-0637R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P38 MAPK Polyclonal Antibody, Unconjugated (bs-0637R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-0637R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated

Catalog #1: bs-0279R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: FITC

Catalog #1: bs-0698R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: HRP

Catalog #1: bs-0465R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Cy7

Catalog #1: bs-0086R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: FITC

Catalog #1: bs-0086R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: HRP

Catalog #1: bs-0766R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bs-0279R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: HRP

Catalog #1: bs-0637R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: APC

Catalog #1: bs-0465R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: HRP

Catalog #1: bs-0412R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: FITC

Catalog #1: bs-0086R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy7

Catalog #1: bs-0086R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Biotin

Catalog #1: bs-0637R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Biotin

Catalog #1: bs-0698R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Cy3

Catalog #1: bs-0698R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Cy7

Catalog #1: bs-0698R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: FITC

Catalog #1: bs-0766R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bs-0766R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: FITC

Catalog #1: bs-0115R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy3

Catalog #1: bs-0115R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy5

Catalog #1: bs-0115R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy7

Catalog #1: bs-0115R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: FITC

Catalog #1: bs-0115R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Biotin

Catalog #1: bs-0115R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: HRP

Catalog #1: bs-0279R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Cy3

Catalog #1: bs-0279R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Cy5

Catalog #1: bs-0279R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Cy7

Catalog #1: bs-0279R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: PE

Catalog #1: bs-0279R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Biotin

Catalog #1: bs-0465R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Cy3

Catalog #1: bs-0465R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Cy5

Catalog #1: bs-0465R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: FITC

Catalog #1: bs-0465R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: PE

Catalog #1: bs-0465R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Biotin

Catalog #1: bs-0412R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Cy3

Catalog #1: bs-0412R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Cy5

Catalog #1: bs-0412R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Cy7

Catalog #1: bs-0412R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Biotin