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Tumor Immunology

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Formalin-fixed and paraffin embedded rat renal tissue labeled with Anti-TGF-beta-1 Polyclonal Antibody, Unconjugated(bs-0086R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Isolated Transforming growth factor beta-1; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Embryo; Lane 2: Rat Cerebrum; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:1000 dilution, overnight at 4˚C. Followed by secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0086R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Unconjugated

Catalog #1: bs-1665R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TGF Beta 1+2+3 Polyclonal Antibody, Unconjugated (bs-4538) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rabbit spleen lysates probed with TGF beta 1+2+3 Polyclonal Antibody, Unconjugated (bs-4538R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
MDA-MB-231 lysates probed with TGF Beta 1+2+3 Polyclonal Antibody, unconjugated (bs-4538R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Mouse Stomach lysates probed with TGF Beta 1+2+3 Polyclonal Antibody, unconjugated (bs-4538R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-4538R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti Arginase II Polyclonal Antibody, Unconjugated (bs-11397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-Arginase II Polyclonal Antibody, Unconjugated (bs-11397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
DU145 Cell lysates; probed with Arginase II Polyclonal Antibody, unconjugated (bs-11397R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Arginase II) polyclonal Antibody, Unconjugated (bs-11397R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-11397R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Sheep

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-IDO Polyclonal Antibody, Unconjugated(bs-15493R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IDO Polyclonal Antibody(bs-15493R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IDO Polyclonal Antibody(bs-15493R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IDO) polyclonal Antibody, Unconjugated (bs-15493R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-15493R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Sheep

Conjugate: Unconjugated

Catalog #1: bs-0086R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: FITC

Catalog #1: bs-0086R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: HRP

Catalog #1: bs-1665R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: FITC

Catalog #1: bs-11397R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Sheep

Conjugate: Biotin

Catalog #1: bs-1665R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: HRP

Catalog #1: bs-15493R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Cy5

Catalog #1: bs-0086R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy7

Catalog #1: bs-0086R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Biotin

Catalog #1: bs-4538R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: FITC

Catalog #1: bs-0086R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy3

Catalog #1: bs-0086R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy5

Catalog #1: bs-11397R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Cy3

Catalog #1: bs-11397R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Cy5

Catalog #1: bs-11397R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Cy7

Catalog #1: bs-11397R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: FITC

Catalog #1: bs-11397R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Sheep

Conjugate: HRP

Catalog #1: bs-1665R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy3

Catalog #1: bs-1665R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy5

Catalog #1: bs-1665R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy7

Catalog #1: bs-1665R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Biotin

Catalog #1: bs-15493R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Cy3

Catalog #1: bs-15493R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Cy7

Catalog #1: bs-15493R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: FITC

Catalog #1: bs-15493R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Sheep

Conjugate: Biotin

Catalog #1: bs-15493R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Sheep

Conjugate: HRP

Catalog #1: bs-4538R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Cy3

Catalog #1: bs-4538R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Cy5

Catalog #1: bs-4538R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Cy7

Catalog #1: bs-4538R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Biotin

Catalog #1: bs-4538R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-1665R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy5.5

Catalog #1: bs-0086R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy5.5

Catalog #1: bs-11397R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Cy5.5

Catalog #1: bs-15493R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Cy5.5

Catalog #1: bs-4538R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-15493R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Sheep

Conjugate: PE

Catalog #1: bs-0086R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PE

Catalog #1: bs-4538R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: PE

Catalog #1: bs-1665R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 350

Catalog #1: bs-1665R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 405

Catalog #1: bs-1665R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 488

Catalog #1: bs-1665R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 555

Catalog #1: bs-1665R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: AbBy Fluor® 594