A Raf(Ser299) Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-3371R-A647
Product Name A Raf(Ser299) Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation ALEXA FLUOR® 647
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser299
Modification Site Ser299
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 369
Swiss Prot P10398
Subcellular location Cytoplasm
Synonyms A RAF phospho S299; p-A RAF phospho S299;A raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 murine sarcoma 3611 virus; ARAF_HUMAN.
Background The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200