{"title":"ELISA Kits","description":"\u003cstyle\u003e\n@media only screen and (max-width: 480px) {\n  video {\n    width: 300px;\n  }\n}\n\u003c\/style\u003e  \n\n\u003cdiv class=\"page\" title=\"Page 3\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"layoutArea\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"column\" data-mce-fragment=\"1\"\u003e\n\u003cdiv title=\"Page 2\" class=\"page\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003cdiv class=\"layoutArea\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003ch3\u003e\u003cspan style=\"color: #0a0b0d;\" data-mce-style=\"color: #0a0b0d;\"\u003eOver 6500 Quality ELISA Kits from 30 Research Areas \u003c\/span\u003e\u003c\/h3\u003e\n\u003ch5\u003e\u003cspan style=\"color: #0a0b0d;\" data-mce-style=\"color: #0a0b0d;\"\u003eBioss supplies research-use Enzyme-Linked Immunosorbent Assay (ELISA) kits that help you confidently detect a variety of antigens or proteins in your samples. We offer a wide selection of species reactivity,including Human, Mouse, Rat, Bovine, Rabbit, Chicken, Porcine, Monkey, Equine, Guinea Pig, and Canine. \u003c\/span\u003e\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ch5\u003e\u003cspan style=\"color: #0a0b0d;\" data-mce-style=\"color: #0a0b0d;\"\u003eHigh Sensitivity with a Broad Assay Range\u003c\/span\u003e\u003c\/h5\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ch5\u003e\u003cspan style=\"color: #0a0b0d;\" data-mce-style=\"color: #0a0b0d;\"\u003e Flexible Inputs: serum, plasma, cell culture supernatant \u003c\/span\u003e\u003c\/h5\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ch5\u003e\n\u003cspan style=\"color: #0a0b0d;\" data-mce-style=\"color: #0a0b0d;\"\u003e\u003c\/span\u003e\u003cspan style=\"color: #0a0b0d;\" data-mce-style=\"color: #0a0b0d;\"\u003e\u003c\/span\u003e\u003cspan style=\"color: #0a0b0d;\" data-mce-style=\"color: #0a0b0d;\"\u003e 48 \u0026amp; 96 Test Sizes Available\u003c\/span\u003e\n\u003c\/h5\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cspan style=\"font-size: 1.4em; color: #111519;\" data-mce-style=\"font-size: 1.4em; color: #111519;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif;\" data-mce-style=\"font-family: -apple-system, BlinkMacSystemFont, 'San Francisco', 'Segoe UI', Roboto, 'Helvetica Neue', sans-serif;\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","products":[{"product_id":"bskm1005","title":"Mouse CXCL1 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-8\/CXCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8\/CXCL1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-8\/CXCL1 is added to the wells and binds to the combination of capture antibody-IL-8\/CXCL1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-8\/CXCL1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8\/CXCL1 standard dilutions and IL-8\/CXCL1 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eMouse KC, also known as CXCL1 or N51, was originally identified in fibroblasts as a PDGFinduced immediate early gene that encodes a secretory protein of approximately 8 kDa. The protein sequence of mouse KC identified it as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines. Besides mitogen-stimulated fibroblasts, KC expression can be induced in bacterial or LPS-stimulated peritoneal and lung macrophages, endothelial cells and vascular smooth cells. The induction of KC by mitogens has been shown to be inhibited by glucocorticoids. \u003cbr\u003e\u003cbr\u003eMouse KC cDNA encodes a 96 amino acid (aa) residue precursor protein from which the aminoterminal 19 aa residues are cleaved to generate the 77 aa residue mature KC. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2, another mouse alpha chemokine. In addition, the protein sequence of KC is approximately 60% identical to the human GROs. Like other alpha chemokines, mouse KC is a potent neutrophil attractant and activator. The activities of KC and MIP-2 have been shown to be mediated by the unique mouse IL-8 receptor that shows 71% and 68% aa sequence identity to human IL-8 Rβ and IL-8 Rα, respectively. Since an IL-8 homolog has not been identified in mice, its has been suggested that MIP-2 and KC are the functional homologs of IL-8 and may function as the major proinflammatory alpha chemokines in mice. Increased KC expression has been found to be associated with neutrophil influx in various inflammatory conditions.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/14825\" target=\"_blank\"\u003e14825\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P12850\" target=\"_blank\"\u003eP12850\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eKC; Fsp; N51; gro; Gro1; Mgsa; Scyb1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-8\/CXCL1(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-8\/CXCL1 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-8\/CXCL1 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7.8pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-8\/CXCL1. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-13, VEGF, LIF, MIP-1, MIP-2, TNF-α, SCF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-2, IL-2 sRα, IL-2sRβ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-8-cxcl1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-8-cxcl1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1005\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-8-cxcl1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-8-cxcl1-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740093071411,"sku":"BSKM1005","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1015","title":"Human Adiponectin ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Adiponectin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Adiponectin present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Adiponectin is added to the wells and binds to the combination of capture antibody- Adiponectin in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of Adiponectin present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Adiponectin standard dilutions and Adiponectin sample concentration determined.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eAdiponectin, alternately named Adipocyte complement-related protein of 30 kDa (Acrp30), adipoQ, adipose most abundant gene transcript 1 (apM1), and gelatin-binding protein of 28 kDa (GBP28), is an adipocyte-specific, secreted protein with potential roles in glucose and lipid homeostasis. Circulating Adiponectin levels are high, accounting for approximately 0.01% of total plasma protein. Adiponectin contains a modular structure that includes an N-terminal collagen-like domain followed by a C-terminal globular domain with significant sequence and structural resemblance to the complement factor C1q. Although they share little sequence identity, similar three-dimensional structure and certain conserved amino acid residues suggest an evolutionary link between the C1q-like domain of Adiponectin and members of the TNF superfamily. Adiponectin assembles into different complexes including trimers (low molecular weight), hexamers (middle molecular weight), and higher order oligomeric structures (high molecular weight) that may affect biological activity. Adiponectin is induced during adipocyte differentiation and its secretion is stimulated by insulin. Two receptors for Adiponectin, termed AdipoR1 and AdipoR2, have been cloned. Although functionally distinct from G-protein-coupled receptors, the genes encode predicted proteins containing 7 transmembrane domains. AdipoR1 is highly expressed in skeletal muscle, while AdipoR2 is primarily found in hepatic tissues. \u003cbr\u003e\u003cbr\u003eInjection of Adiponectin into non-obese diabetic mice leads to an insulin-independent decrease in glucose levels. This is likely due to insulin-sensitizing effects involving Adiponectin regulation of triglyceride metabolism. A truncated form of Adiponectin (gAdiponectin) containing only the C-terminal globular domain has been identified in the blood, and recombinant gAdiponectin has been shown to regulate weight reduction as well as free fatty acid oxidation in mouse muscle and liver. The full-length recombinant Adiponectin protein is apparently less potent at mediating these effects. The mechanism underlying the role of Adiponectin in lipid oxidation may involve the regulation of expression or activity of proteins associated with triglyceride metabolism including CD36, acyl CoA oxidase, AMPK, and PPARγ. \u003cbr\u003e\u003cbr\u003eAlthough Adiponectin-regulation of glucose and lipid metabolism in humans is less clear, similar mechanisms may also be in place. A negative correlation between obesity and circulating Adiponectin has been well established, and Adiponectin levels increase concomitantly with weight loss. Decreased Adiponectin levels are associated with insulin resistance and hyperinsulinemia, and patients with type-2 diabetes are reported to exhibit decreased circulating Adiponectin. Thiazolidinediones, a class of insulin-sensitizing, anti-diabetic drugs, elevate Adiponectin in insulin-resistant patients. In addition, high Adiponectin levels are associated with a reduced risk of type-2 diabetes. Using magnetic resonance spectroscopy it has been demonstrated that intracellular lipid content in human muscle negatively correlates with Adiponectin levels, potentially due to Adiponectin-induced fatty acid oxidation. \u003cbr\u003e\u003cbr\u003eAdiponectin may also play anti-atherogenic and anti-inflammatory roles. Adiponectin plasma levels are decreased in patients with coronary artery disease. Furthermore, neointimal thickening of damaged arteries is exacerbated in Adiponectin-deficient mice and is inhibited by exogenous Adiponectin. Adiponectin inhibits endothelial cell expression of adhesion molecules in vitro, suppressing the attachment of monocytes. In addition, Adiponectin negatively regulates myelomonocytic progenitor cell growth and TNF-α production in macrophages.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/9370\" target=\"_blank\"\u003e9370\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q15848\" target=\"_blank\"\u003eQ15848\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human Adiponectin (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Adiponectin Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human Adiponectin monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 bottles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 15pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e62.5 - 4000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human Adiponectin. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4-1BB, APRIL, BAFF\/BLyS, CD27, CD30 Ligand, CD40 Ligand, Fas Ligand, GITR Ligand, LIGHT, OX40 Ligand, TNF-α, TNF-β, TRAIL, TRANCE, TWEAK, VEGI, LT-α1\/β2, LT-α2\/β1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eAdiponectin, CD27 Ligand, Cd30 Ligand, Fas Ligand, LT-α1\/β2, LT-α2\/β1, OX40 Ligand, TNF-α, TNF-α(truncated), TRANCE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ePorcine TNF-α, Rat TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-adiponectin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-adiponectin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1015.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-adiponectin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-adiponectin-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097396787,"sku":"BSKH1015","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1032","title":"Human FGF basic ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for FGF basic has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF basic present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for FGF basic is added to the wells and binds to the combination of capture antibody- FGF basic in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of FGF basic present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven FGF basic standard dilutions and FGF basic sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eFGF basic, also called FGF-2 (fibroblast growth factor-2) or HBGF-2 (heparin-binding growth factor-2), is the most intensively studied of the 22 mitogenic proteins of the FGF family. Family members share 35 - 60% amino acid (aa) identity, but only FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. \u003cbr\u003e\u003cbr\u003eThe 18 kDa FGF basic isoform can be found in both the cytoplasm and the nucleus and is also the form that is secreted. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. Transcription from alternate start sites produces 21-23 kDa forms found only in the nucleus. High and low molecular weight human FGF basic isoforms target the expression of different genes. The 18 kDa human FGF basic sequence shares 97% and 99% aa identity with mouse\/rat and bovine\/ovine FGF basic, respectively. Expression of FGF basic is nearly ubiquitous. However, disruption of the mouse FGF basic gene gives relatively mild cardiovascular, skeletal, and neuronal phenotypes, suggesting compensation by other FGF family members. Transgenic over-expression of FGF basic mainly influences development and mineralization of bone.\u003cbr\u003e\u003cbr\u003eFour FGF tyrosine kinase receptors (FGF R) and their splice variants show differential binding of FGFs. FGF basic preferentially binds FGF R1c and 2c, for which it has picomolar affinity. FGF basic also has a number of other binding partners that fine-tune FGF basic activities, according to their locations and quantities. FGF basic modulates such normal processes as angiogenesis, wound healing, tissue repair, learning and memory, and embryonic development and differentiation of heart, bone and brain. It is upregulated in response to inflammation via mediators such as TNF-α, IL-1β, IL-2, PDGF, and nitric oxide. Many human tumors express FGF basic, which may correlate with tumor vascularity.\u003c\/p\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/2247\" target=\"_blank\"\u003e2247\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P09038\" target=\"_blank\"\u003eP09038\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eBFGF; FGFB; FGF-2; HBGF-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human FGF basic (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman FGF basic Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human FGF basic monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8 - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human FGF basic. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, LIF, EGF, TGF-β1, TNF-α, TNF-β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, EGF, GM-CSF, TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ehuman PDGF, porcine PDGF, human TGF-β1, porcine TGF-β1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-fgf-basic-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-fgf-basic-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1032.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-fgf-basic-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-fgf-basic-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097462323,"sku":"BSKH1032","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1045","title":"Human GDF-15 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GDF-15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDF-15 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GDF-15 is added to the wells and binds to the combination of capture antibody- GDF-15 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of GDF-15 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven GDF-15 standard dilutions and GDF-15 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eGrowth and differentiation factor-15 (GDF-15), also known as macrophage inhibitory cytokine-1(MIC-1), placental transforming growth factor-β, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-β) superfamily. GDF-15 is initially synthesized as a 40 kDa inactive precursor protein. It is then proteolytically cleaved to release the active C-terminal fragment, which is assembled into a disulfide-linked homodimer of 28 kDa to become biologically active GDF-15. In humans, GDF-15 is predominantly expressed in the placenta, with low levels in the kidney, pancreas, and prostate. However, its expression can be rapidly induced by cytokines such as interleukin-1 and TGF-β.\u003cbr\u003e\u003cbr\u003eGDF-15 has diverse biological functions. Early studies have shown that low serum GDF-15 levels correlate with miscarriages, indicating that it might be able to suppress inflammation in early pregnancy. GDF-15 also plays an important role in tumorigenesis and metastasis. It has been observed that in many types of cancers, such as colorectal, breast, and prostate, the expression of GDF-15 is dramatically increased. Additionally, in cancer patients, serum levels of GDF-15 are elevated, which are of value in disease diagnosis and stratification. GDF-15 is strongly induced by the tumor suppressor gene p53 and other anti-tumorigenic agents, such as the non-steroidal anti-inflammatory drugs and peroxisome proliferators-activated receptor γ. These findings suggest that GDF-15 may be a downstream target of those signaling pathways that regulate cell cycle arrest and apoptosis. Through the modulation of neuronal pathways important in the regulation of appetite and energy homeostasis, GDF-15 mediates cancer-induced anorexia and weight loss.\u003c\/p\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/9518\" target=\"_blank\"\u003e9518\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q99988\" target=\"_blank\"\u003eQ99988\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ePDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human GDF-15 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman GDF-15 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human GDF-15 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8 - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human GDF-15. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eGDF-11, GDF-5, GDF-6, GDF-7, GDF-8, EGF, CSF, sTNF-RII, FGF, TNF-α, PDGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eGDF-11, GDF-5, GDF-6, GDF-7, GDF-8, GDF-9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-gdf-15-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-gdf-15-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1045.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-gdf-15-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-gdf-15-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097495091,"sku":"BSKH1045","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1018","title":"Human GM-CSF ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GM-CSF is added to the wells and binds to the combination of capture antibody- GM-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of GM-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven GM-CSF standard dilutions and GM-CSF sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eGranulocyte macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine with multiple effects on hematopoietic cells. It mobilizes CD34+ progenitor cells into the periphery and stimulates their proliferation, survival and differentiation into neutrophils, monocytes\/ macrophages, eosinophils, and myeloid dendritic cells. On these terminally differentiated myeloid cells, GM-CSF is also needed for inducing their effector functions. In addition, GM-CSF has been shown to stimulate the proliferation and differentiation of the erythroid and megakaryocyte progenitor cells. \u003cbr\u003e\u003cbr\u003eGM-CSF is produced by a number of different cell types, including keratinocytes, mature and immature NK cells, type II alveolar cells), endothelial cells, monocytes, bone-marrow mesenchymal stem cells, CD4+ and CD8+ T cells, megakaryocytes, B cells, eosinophils, chondrocytes and fibroblasts.\u003cbr\u003e\u003cbr\u003eHuman GM-CSF cDNA encodes a 144 amino acid (aa) residue precursor protein with a 17 aa putative signal peptide and a 127 aa mature protein. Natural GM-CSF is a monomer that contains both N- and O-linked glycosylation. Mature human GM-CSF shares approximately 55%, 63% and 68% aa sequence homology with mouse, rat and canine. GM-CSF, respectively. Human GM-CSF is not biologically active on mouse cells, but was reported to have some activity on canine cells. \u003cbr\u003e\u003cbr\u003eGM-CSF exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins. The presence of the spliced variants in the heteromeric receptor complex can regulate the functions of the complex.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/1437\" target=\"_blank\"\u003e1437\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P04141\" target=\"_blank\"\u003eP04141\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCSF; GMCSF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human GM-CSF (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman GM-CSF Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human GM-CSF monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6 - 1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human GM-CSF. The factors listed below were prepared at 10ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, M-CSF, FGF-4, FGF-5, FGF-6, IL-1α, IL-1β, IL-3, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, VEGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eGM-CSF, IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ebovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, Human TGF-β1, porcine TGF-β1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-gm-csf-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-gm-csf-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1018.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-gm-csf-elisa-kit.assay\"\u003ehttps:\/\/www.myassays.com\/bioss-human-gm-csf-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097527859,"sku":"BSKH1018","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1013","title":"Human IFN-γ ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-γ is added to the wells and binds to the combination of capture antibody- IFN-γ in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IFN-γ present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-γ standard dilutions and IFN-γ sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterferon gamma (IFN-γ) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA. Produced by Tlymphocytes and natural killer (NK) cells, IFN-γ is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-γ is reported to be active only on human and non-human primate cells. The biochemistry and biological activities of interferons have been extensively reviewed.\u003cbr\u003e\u003cbr\u003eHuman IFN-γ is a 143 amino acid residue, 20 or 25 kDa glycoprotein that demonstrates little sequence homology to IFN-α or -β. Naturally occurring IFN-γ is found as either of two molecular weight species, differing in degree of glycosylation. Human IFN-γ apparently exists as a head-to-tail dimer in solution with the C-terminus of one monomer aligned with the N-terminus of the other monomer A receptor for IFN-γ has been identified and its gene localized to chromosome 6 Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-γ.\u003cbr\u003e\u003cbr\u003eFunctionally, IFN-γ produces a variety of effects. Produced by CD8+, NK, gd, and TH1 T helper cells, IFN-γ has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes he antiprotozoal activity of IFN-γ against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-γ .The immunomodulatory effects of IFN-γ are extensive and diverse. In monocyte\/macrophages, the activities of IFN-γ include: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production.Finally, IFN-γ has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.\u003c\/p\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3458\" target=\"_blank\"\u003e3458\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P01579\" target=\"_blank\"\u003eP01579\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIFG, IFI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IFN-γ(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IFN-γ Standard lyophilized, 4000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IFN-γ monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6 - 1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IFN-γ. The factors listed below were prepared at 10ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eEGF, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, MCP-1, TGF-β1, TGF-β2, TGF-β3, TGF-β5, VEGF, TNF-α, TNF-β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-α, IFN-γ, IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, GM-CSF, LIF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-ifn-%3f-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-ifn-%3f-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1013.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-ifn-%3f-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-ifn-%3f-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097560627,"sku":"BSKH1013","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1001","title":"Mouse IFN-γ ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-γ is added to the wells and binds to the combination of capture antibody-IFN-γ in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IFN-γ present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-γ standard dilutions and IFN-γ sample concentration determined..\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterferon-gamma (IFN-γ) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA. Produced by T-lymphocytes and natural killer (NK) cells, IFN-γ is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-γ is reported to be active only on human and non-human primate cells. The biochemistry and biological activities of interferons have been extensively reviewed.\u003cbr\u003e\u003cbr\u003eMouse IFN-γ encodes a 155 amino acid (aa) residue precursor protein with a hydrophobic signal peptide that is cleaved to generate the 133 aa residue mature protein. In solution, IFN-γ has been shown to exist as a non-covalently associated homodimer with topological similarity to IL-10. Mouse IFN-γ shows approximately 40% aa sequence identity to human IFN-γ and there is no cross-reactivity across species.\u003cbr\u003e\u003cbr\u003eA receptor for IFN-γ has been identified and its gene localized to chromosome 6 Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-γ.\u003cbr\u003e\u003cbr\u003eFunctionally, IFN-γ produces a variety of effects. Produced by CD8+, NK, gd, and TH1 T helper cells, IFN-γ has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes he antiprotozoal activity of IFN-γ against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-γ. The immunomodulatory effects of IFN-γ are extensive and diverse. In monocyte\/macrophages, the activities of IFN-γ include: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production. Finally, IFN-γ has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.\u003c\/p\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/15978\" target=\"_blank\"\u003e15978\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P01580\" target=\"_blank\"\u003eP01580\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIFN-g, Ifg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminum pouches with a Microwell Plate coated with monoclonal antibody to mouse IFN-γ(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IFN-γStandard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IFN-γmonoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8 - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IFN-γ. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, C10, TNF-α, IL-1α, IL-1β, IL-2 , IL-3, IL-5, IL-6, IL-7, IL-9, LIF, M-CSF, SCF, VEGF, MIP-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-ifn-%3f-elisa-kit.assay\" target=\"_blank\"\u003eMyAssays\u003c\/a\u003e to offer you an easy-to-use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-ifn-%3f-elisa-kit.assay\" target=\"_blank\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1001\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-ifn-%3f-elisa-kit.assay\" target=\"_blank\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-ifn-%3f-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097593395,"sku":"BSKM1001","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1063","title":"Human IgG ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IgG has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any IgG present is bound by the immobilized antibody and the detection antibody specific for IgG is binds to the combination of capture antibody-IgG in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IgG present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IgG standard dilutions and IgG sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures .\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eImmunoglobulin G (IgG) is a type of antibody. Representing approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG has two antigen binding sites. IgG are involved in the regulation of allergic reactions. According to Finkelman, there are two pathways of systemic anaphylaxis: antigens can cause systemic anaphylaxis in mice through classic pathway by cross-linking IgE bound to the mast cell receptor FcεRI, stimulating the release of both histamine and platelet activating factor (PAF). In the alternative pathway antigens form complexes with IgG, which then cross-link macrophage receptor FcγRIII and stimulates only PAF release. Human IgG is the major immunoglobulin in blood and plays a key role in the humoral immune response.\u003c\/p\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with antibody to human IgG-HRP (812)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IgG standard lyophilized, 50000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAntibody to human IgG-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 bottles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAntibody to human IgG-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 390pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e780 - 50000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IgG.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-igg-elisa-kit.assay\" target=\"_blank\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-igg-elisa-kit.assay\" target=\"_blank\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1063.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-igg-elisa-kit.assay\" target=\"_blank\"\u003ehttps:\/\/www.myassays.com\/bioss-human-igg-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097658931,"sku":"BSKH1063","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1010","title":"Human IL-10 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-10 is added to the wells and binds to the combination of capture antibody- IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (TH2) clones that suppressed the production of cytokines by T helper 1 (TH1) clones responding to antigen stimulation in the presence of monocyte\/macrophage antigen-presenting cells.\u003cbr\u003e\u003cbr\u003eThe human homologue of mouse IL-10 was subsequently cloned by cross-hybridization. Human and mouse IL-10 are 81% and 73% identical at the nucleotide and amino acid levels, respectively. Mouse, rat and human IL-10 cDNAs encode 178 amino acid (aa) residue precursor polypeptides with hydrophobic signal peptides that are cleaved to generate 160 aa residue mature proteins. There is at least 73% aa identity between the proteins from the three species. In contrast to mouse IL-10, human IL-10 appears to be non-glycosylated. Both mouse and human IL-10 exist as non-covalently-linked homodimers in solution. Although human IL-10 is active on mouse cells, mouse IL-10 does not show species cross-reactivity on human cells.\u003cbr\u003e\u003cbr\u003eIL-10 is a pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. human IL-10 can inhibit monocyte\/macrophage-dependent, antigen-stimulated cytokine synthesis by human PBMNC and NK cells. L-10 can inhibit production or function of a macrophage membrane-bound costimulator required for activation of T cells and NK cells.\u003cbr\u003e\u003cbr\u003eIL-10 is a potent modulator of monocyte\/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7. Finally, IL-10 has been reported to suppress the synthesis of superoxide anion plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages. IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4, and on thymocytes and mast cells, where it acts as a growth costimulator.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3586\" target=\"_blank\"\u003e3586\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P22301\" target=\"_blank\"\u003eP22301\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCSIF; TGIF; GVHDS; IL-10; IL10A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-10 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-10 Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-10 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8 - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-10. The factors listed below were prepared at 100ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-12, IL-12p40, IL-17, Leptin, IFN-γ, TNF-α, TNF-β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-12p40, IL-17, Leptin, IFN-γ, TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant rat\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-4, IL-6, Leptin, TNF-α, IFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1010.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-10-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097921075,"sku":"BSKH1010","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1007","title":"Mouse IL-10 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-10 is added to the wells and binds to the combination of capture antibody-IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 10 (IL-10), initially designated cytokine synthesis inhibitor, is a pleiotropic cytokine that inhibits the production of a number of cytokines (including IL-1, GM-CSF, TNF, IL-6, IL-8, IL-12 and IFN-γ) by activated Th1 cells, NK cells, and monocytes\/macrophages. \u003cbr\u003e\u003cbr\u003eMouse IL-10 is produced by a wide variety of cells, including activated Th2 cells, fetal thymocytes, monocytes\/macrophages, keratinocytes, B cells, and glial cells. Mouse IL-10 cDNA encodes a 178 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to generate the 160 amino acid residue mature protein. At the amino acid sequence level, there is approximately 73% identity between mouse and rat or mouse and human IL-10. Although human IL-10 is active on mouse cells, mouse IL-10 does not show species cross-reactivity on human cells. Herpes viruses, including EBV and equine herpes virus type 2, have been shown to encode viral homologues of IL-10 that exhibit some of the activities of IL-10 on both mouse and human cells.\u003cbr\u003e\u003cbr\u003eIL-10 has been shown to inhibit macrophage cytotoxic activity and to stimulate the proliferation and differentiation of B cells, mast cells, and thymic T cells. IL-10 is a potent modulator of monocyte\/macrophage function. IL-10 also enhances the release of soluble TNF receptors and inhibits the expression of surface ICAM-1 and B7. Finally, IL-10 has been reported to suppress the synthesis of superoxide anion plus reactive oxygen intermediates (ROI), and either inhibit or facilitate nitric oxide synthesis, depending on the time of exposure to activated macrophages. IL-10 has marked effects on B cells. IL-10 also has documented activity on endothelial cells, where it mimics IL-4, and on thymocytes and mast cells, where it acts as a growth costimulator.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16153\" target=\"_blank\"\u003e16153\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P18893\" target=\"_blank\"\u003eP18893\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCSIF; Il-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-10(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-10 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-10 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-10. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-3, IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant porcine\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1007\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-10-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097953843,"sku":"BSKM1007","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskr1014","title":"Rat Rat Interleukin 10 (IL-10) ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-10 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any IL-10 present is bound by the immobilized antibody and the detection antibody specific for IL-10 is binds to the combination of capture antibody-IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 α-helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26\/AK155. IL-10 is a pleiotropic cytokine that exerts immunosuppressive or immunostimulatory effects in a variety of cell types. In 1989, Fiorentino et al. found that the mouse Th2 cell line D10.G4.1 produced a new cytokine that inhibits the transcription of cytokine mRNA in Th1 cell line, called cytokine synthesis inhibitory factor (CSIF) and named IL-10 in the same year.\u003cbr\u003e\u003cbr\u003eMouse, rat and human IL-10 cDNA sequences each contain 178 amino acid residues and have an 18 amino acid signal peptide sequence. The mature IL-10 molecule is 160 amino acid residues, and the mouse and human IL-10 molecules contain 5 and 4 cysteine residues respectively. Their molecular weight ranges from 35-40 kDa. At the amino acid level, rat IL-10 has 85% and 74% homology to human and mouse IL-10 respectively. Rat and human IL-10 act on mouse-derived cells, whereas mouse IL-10 does not cross-react to human cells.\u003cbr\u003e\u003cbr\u003eIL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 Rα and IL-10 Rβ. IL-10 Rα is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells, while the 75 kDa transmembrane IL-10 Rβ is widely expressed. The IL-10 dimer binds to two IL-10 Rα chains, triggering recruitment of two IL-10 Rβ chains. IL-10 Rβ does not bind IL-10 directly but is required for signal transduction. IL-10 Rβ also associates with IL-20 Rα, IL-22 Rα1, or IL-28 Rα to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.\u003cbr\u003e\u003cbr\u003eAntagonists of IL-10 may have anti-EB virus effects; IL-10 may become an anti-inflammatory treatment by promoting the expression of IL-1 Rα by monocytes. Animal experiments show that IL-10 can effectively prevent the death of mouse shock induced by LPS.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/25325\" target=\"_blank\"\u003e25325\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P29456\" target=\"_blank\"\u003eP29456\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL10X\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with antibody to rat IL-10 (8-12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRat IL-10 standard lyophilized, 2000pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-rat IL-10 antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003estandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 15pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e31.25 - 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant Rat IL-10. The factors listed below were prepared at 50ng\/ml in standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant rat\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCINC-1, GDNF, GM-CSF, IFN-γ, β-NGF, PDGF-BB, TNF-α, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-18\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-10, IL-10sR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-10 sR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKR1014\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-il-10-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-rat-il-10-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740097986611,"sku":"BSKR1014","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1020","title":"Mouse IL-12\/23(P40) ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12\/IL-23p40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12\/IL-23p40 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12\/IL-23p40 is added to the wells and binds to the combination of capture antibody- IL-12\/IL-23p40 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-12\/IL-23p40 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12\/IL-23p40 standard dilutions and IL-12\/IL-23p40 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eIL-12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine produced primarily by antigen-presenting cells (monocytes\/macrophages, dendritic cells and B lymphocytes). IL-12 has multiple effects on T lymphocytes and natural killer (NK) cells, including the ability to stimulate cytotoxicity, proliferation, cytokine production and Th1 subset development. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a unique 35 kDa (p35) subunit and a common 40 kDa (p40) subunit that is also present in IL-23. Monomers of the p40 and p35 subunits by themselves do not have IL-12 activity, but the homodimer of p40 has been shown to bind the IL-12 receptor and is an IL-12 antagonist. In cells expressing both p35 and p40 mRNAs, p40 mRNA is expressed to a higher level and free p40 subunits not associated with p35 subunits are secreted together with heterodimeric IL-12 p70. Most of the free p40 subunits secreted by the various human cell lines examined have been found to exist as monomers. In the culture supernatants of various activated human monocytes where free p40 is present in vast excess over p70, the levels of p70 measured by bioassays are consistent with those measured using a p70-specific immunoassay, suggesting that p40 monomers are not efficient IL-12 antagonists. In the mouse system, p40 homodimers are produced in vivo and function as IL-12 antagonists. Polymorphisms exist in the mouse IL-12\/IL-23 p40 sequence.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16160\" target=\"_blank\"\u003e16160\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P43432%20\" target=\"_blank\"\u003eP43432 \u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ep40; Il-12b; Il12p40; Il-12p40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-12\/IL-23p40 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-12\/IL-23p40 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-12\/IL-23p40 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8-500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-12\/IL-23p40. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2 , IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, LIF, TNF-α, IFN-γ, MIP-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-12\/IL-23 p40 (dimer), IL-12 p70\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-2-23(p40)-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-2-23(p40)-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1020\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-2-23(p40)-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-12-23(p40)-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098052147,"sku":"BSKM1020","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1017","title":"Mouse IL-12p70 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12p70 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12p70 is added to the wells and binds to the combination of capture antibody- IL-12p70 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-12p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12p70 standard dilutions and IL-12p70 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of a human B-lymphoblastoid cell line. \u003cbr\u003e\u003cbr\u003eBiologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. While the p40 and p35 subunits by themselves do not have IL-12 activity, the p40 homodimer has been shown to bind the IL-12 receptor and is an IL-12 antagonist. Mature mouse p35 subunit is composed of 193 amino acid (aa) residues and contains seven cysteines plus one potential N-linked glycosylation site. Mature mouse p40 subunit has 313 aa, with 13 cysteines and five potential N-linked glycosylation sites. Mouse p35 and p40 subunits show 63% and 72% aa identity, respectively, to the human p35 and p40 subunits. Although mouse IL-12 is active on both human and mouse cells, human IL-12 is only active on human cells. \u003cbr\u003e\u003cbr\u003eIL-12 has been shown to have multiple effects on T lymphocytes and natural killer (NK) cells. Some of these effects include the induction of IFN-γ and TNF production by T and NK cells, the enhancement of cytotoxic activity of T and NK cells and the stimulation of T and NK cell proliferation. IL-12 has also been shown to be a central mediator of the cell-mediated immune response by promoting Th1 development.\u003cbr\u003e\u003cbr\u003eCell surface staining for IL-12 on a human monocytic and mouse macrophage cell line has been reported, suggesting that membrane-associated IL-12 may exist. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Although a human B cell line has been shown to produce IL-12, fresh B cells are apparently not producers of IL-12.\u003c\/p\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-12p70 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-12p70 Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-12p70 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-12p70. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2 , IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p35, IL-12p40 monomer, IL-12p70, IL-23, TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, IL-6, IL-6sR, IL-12p35, IL-12p40 monomer, IL-12p70, IL-23, GM-CSF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-12p70-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-12p70-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1017\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-12p70-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-12p70-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098150451,"sku":"BSKM1017","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1006","title":"Mouse IL-13 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-13 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-13 is added to the wells and binds to the combination of capture antibody-IL-13 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-13 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-13 standard dilutions and IL-13 sample concentration determined. \u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eIL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23\/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1\/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16163\" target=\"_blank\"\u003e16163\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P20109%20\" target=\"_blank\"\u003eP20109 \u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eInterleukin-13, Il-13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-13(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-13 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-13 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8-500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-13. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-9, IL-10, VEGF, KC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-4, IL-4 sRα, IL-13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-13-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-13-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1006\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-13-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-13-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098183219,"sku":"BSKM1006","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1029","title":"Human IL-15 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-15 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-15 is added to the wells and binds to the combination of capture antibody- IL-15 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-15 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-15 standard dilutions and IL-15 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 15 (IL-15) is a 14 kDa cytokine that is structurally and functionally related to IL- 2. Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly. IL-15 associates with IL-15 Rα in the endoplasmic reticulum, and this complex is expressed on the cell surface. The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 Rα are coordinately expressed on the surface of one cell and interact with complexes of IL-2 Rβ\/γc on adjacent cells. This enables cells to respond to IL-15 even if they do not express IL-15 Rα. Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis. It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells. IL-15 also exerts pleiotropic effects on other hematopoietic cells and nonimmune cells. Ligation of membrane associated IL-15\/IL-15Rα complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15\/IL-15Rα expressing cells.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3600\" target=\"_blank\"\u003e3600\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P40933%20\" target=\"_blank\"\u003eP40933 \u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-15 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-15 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-15 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 2pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.81 - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-15. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-12, IL-13, MIP-1α, MIP-1β, IFN-γ, TNF-α, TNF-β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-13, MIP-1α, MIP-1β, SCF, TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ebovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-β1, porcine TGF-β1, bovine FGF acidic, bovine FGF basic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-15-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-15-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1029.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-15-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-15-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098215987,"sku":"BSKH1029","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1028","title":"Human IL-17 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-17 is added to the wells and binds to the combination of capture antibody- IL-17 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-17 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-17 standard dilutions and IL-17 sample concentration determined\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eIL-17, originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8), is produced by activated T lymphocytes, primarily by memory T cells. IL-17 appears to mediate communication between the immune system and the hematopoietic system.\u003cbr\u003e\u003cbr\u003eIL-17 is a disulfide-linked homodimer. Each polypeptide has 155 amino acid (aa) residues (predicted mass = 17.5 kDa), including a 19 aa residue hydrophobic leader sequence. There are six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins. The aa sequence of human IL-17 is 63% and 58% identical to mouse and rat IL-17 and 72% identical to the thirteenth ORF of Herpesvirus saimiri. There is at least some species specificity for in vitro action on bone-marrow stromal cells.\u003cbr\u003e\u003cbr\u003eIL-17 mediation of T cell communication with the hematopoietic system is suggested by two observations. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-ЌB-mediated mechanism. IL-6 in turn promotes development of granulocyte\/macrophage colonies, and G-CSF directs development of neutrophils. IL-17 also enhances proliferation of partially activated T cells and upregulates nitric oxide (NO) production in osteoarthritic cartilage.\u003c\/p\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3605\" target=\"_blank\"\u003e3605\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q16552\" target=\"_blank\"\u003eQ16552\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL17; CTLA8; IL-17; CTLA-8; IL-17A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-17 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-17 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-17 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6 - 1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-17. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-17B, IL-17C, IL-17D, IL-17F, IFN-γ, IL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-17\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-17-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-17-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1028.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-17-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-17-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098248755,"sku":"BSKH1028","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1021","title":"Mouse IL-17A ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17A present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-17A is added to the wells and binds to the combination of capture antibody-IL-17A in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-17A present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-17A standard dilutions and IL-17A sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eThe IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16171\" target=\"_blank\"\u003e16171\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q62386\" target=\"_blank\"\u003eQ62386\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIl17; Ctla8; IL-17; Ctla-8; IL-17A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-17A(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-17A Standard lyophilized, 500 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-17A monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8-500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-17A. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2 , IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, LIF, Leptin, TNF-α, VEGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-17\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-17a-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-17a-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1021\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-17a-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-17a-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098281523,"sku":"BSKM1021","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1056","title":"Human IL-18 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-18 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any IL-18 present is bound by the immobilized antibody and the detection antibody specific for IL-18 is binds to the combination of capture antibody-IL-18 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-18 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-18 standard dilutions and IL-18 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 18 (IL-18) is an 18 kDa cytokine which is identified as a costimulatory factor for production of interferon-r (IFN-r) in response to toxic shock. It shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1b converting enzyme (ICE, caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It has been reported that IL-18 is produced from dendritic cells, activated macrophages, Kupffer cells, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalons. IFN-r is produced by activated T and NK cells and plays critical roles in the defense against microbial pathogens. IFN-g activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens expression, and inhibits osteoclast activation. IL-18 acts on T helper 1-type (Th1) cells, and in combination with IL-12 strongly induces production of IFN-g by these cells. Pleiotropic effects of IL-18 have also been reported, including enhancement production of IFN-g and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-g in T cells, enhancement of Fas ligand expression by Th1 cells.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3606\" target=\"_blank\"\u003e3606\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q14116%20\" target=\"_blank\"\u003eQ14116\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIGIF; IL-18; IL-1g; IL1F4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with antibody to human IL-18 (812)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-18 standard lyophilized, 2500 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-18 antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 20pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e39 - 2500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-18. The factors listed below were prepared at 50ng\/ml in standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIFN-γ, IL-10, IL-12, IL-16, IL-17B, IL-17C, IL-17D, IL-17F\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-17\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-18-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-18-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for BSKH1056.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-18-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-18-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098314291,"sku":"BSKH1056","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1001","title":"Human IL-1ß ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1β present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1β is added to the wells and binds to the combination of capture antibody- IL-1β in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-1β present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1β standard dilutions and IL-1β sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin-1 (IL-1), originally described as lymphocyte activating factor (LAF) for its effects on thymocytes, is a polypeptide cytokine with two molecular forms. The two distinct molecular forms of IL-1 are thought to be derived from two genes. After transcription, as 31kD precursor polypeptide is cleaved to give rise to mostly cell membrane associated IL-1α and secreted IL-1β. Both have the same molecular weight of 15kD but have different isoelectric points of 5 and 7, respectively. \u003cbr\u003e\u003cbr\u003eDespite sequence homology of only 20%, both forms are thought to bind to the same receptor. IL-1 inhibitors that vary only in their degree of glycosylation have been described to bind to the IL-1 receptor. These inhibitors are structurally related to IL-1β and may be important in regulation of IL-1β action.\u003cbr\u003e\u003cbr\u003eIL-1β is produced primarily by monocytes and macrophages but also by astrocytes, oligodendroglia, adrenal cortical cells, NK cells, endothelial cells, keratinocytes, megakaryocytes, platelets, neurons, neutrophils, osteoblasts, Schwann cells, trophoblasts, T cells, and fibroblasts. IL-1 has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes. A true pleiotrope, IL-1 may have tumoricidal activity via its release of IL-2 and Interferon gamma and indirectly antiviral by stimulating to release interferon beta.\u003cbr\u003e\u003cbr\u003eLow levels of IL-1β have been reported in normal serum. It is thought that IL-1 genes are induced to respond to tissue damage or infection. Elevated levels have been reported in a number of infectious disease conditions and in noninfectious inflammatory conditions such as Crohn’s disease. In addition to elevated serum levels, IL-1 has been found in synovial fluids of patients with rheumatoid arthritis and in cerebrospinal fluid after neurological inflammation or insult. At the other end of the spectrum, low levels of IL-1 have been found in malnutrition and advanced neoplasia suggesting perhaps a complex immunological and physiological regulatory role for this cytokine.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3553\" target=\"_blank\"\u003e3553\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P01584\" target=\"_blank\"\u003eP01584\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eInterleukin-1 beta; IL1B; L1F2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-1β(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-1β Standard lyophilized, 500 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-1βmonoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay is less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose is 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.81- 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-1β. The factors listed below were prepared at 50ng\/ml in Standard \/samples. Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1ra, IL-1 sRI, IL-1 sRII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant porcine\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e:IL-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-1%ce%b2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-1%ce%b2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1001\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-1%ce%b2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-1%ce%b2-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098347059,"sku":"BSKH1001","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1015","title":"Mouse IL-1ß ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1β has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any IL-1β present is bound by the immobilized antibody and the detection antibody specific for IL-1β is binds to the combination of capture antibody- IL-1β in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-1β present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-18 standard dilutions and IL-1β sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 1 (IL-1) is a name that designates two proteins, IL-1αand IL-1β, which are the products of distinct genes, but which recognize the same cell surface receptors. With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, IL-1 is not produced by the cells of healthy individuals. However, in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen.\u003cbr\u003e\u003cbr\u003eIL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. \u003cbr\u003e\u003cbr\u003eIntracellular IL-1βconsists exclusively of the 31 kDa precursor form. Extracellular IL-1βconsists of a mixture of both unprocessed and mature IL-1β. These results indicate that processing takes place subsequently to secretion and is not tightly coupled to secretion. The specific protease apparently responsible for the processing of IL-1β, designated interleukin-1β-converting enzyme (ICE), has been described.\u003cbr\u003e\u003cbr\u003eIL-1 possesses a wide variety of biological activities. IL-1 also plays an important role in immune functions, having effects on macrophages \/ onocytes, Tlymphocytes, B lymphocytes, NK cells, and LAK cells. It acts on macrophages\/ monocytes, inducing its own synthesis as well as the production of TNF and IL-6. It activates T cells, resulting in IL-2 production and expression of IL-2 receptors. IL-1 also induces the production of GM-CSF and IL-4 from activated T cells. It induces B cell proliferation and maturation and increased immunoglobulin synthesis.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16176\" target=\"_blank\"\u003e16176\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P10749\" target=\"_blank\"\u003eP10749\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIl-1b; IL-1beta\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with antibody to mouse IL-18 (812)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-1β standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-1β antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-1β. The factors listed below were prepared at 50ng\/ml in standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1ra, IL-1 RI, IL-1 RII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant porcine\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-1%ce%b2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-1%ce%b2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1015\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-1%ce%b2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-1%ce%b2-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098412595,"sku":"BSKM1015","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1002","title":"Human IL-2 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-4 is added to the wells and binds to the combination of capture antibody- IL-4 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-4 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-4 standard dilutions and IL-4 sample concentration determined.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils. The sequence of human IL-4 cDNA predicts a 153 amino acid (aa) residue precursor protein containing a 24 aa residue signal peptide that is cleaved to form the mature protein. At the amino acid sequence level, mature human IL-4 is approximately 50% identical to mouse IL-4 and there is no species cross-reactivity between the two proteins. Human IL-4 also shares approximately 30% amino acid sequence identity to human IL-13 and the two cytokines exhibit overlapping biological activities. The gene for IL-4 has been mapped to human chromosome 5q, in close proximity to the genes for IL-3, IL-5, IL-13 and GM-CSF.\u003cbr\u003e\u003cbr\u003eIL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the TH2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro.\u003cbr\u003e\u003cbr\u003eThe biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit (IL-4 R) and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-2, IL-7, IL-9 and IL-15. A second type of functional IL-4 receptor complex, consisting of the IL-4 R and the more recently cloned IL-13 R, has also been proposed.\u003cbr\u003e\u003cbr\u003eAlthough IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13. In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernatants. Soluble IL-4 R has been shown to bind IL-4 with high affinity in solution.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3565\" target=\"_blank\"\u003e3565\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P05112\" target=\"_blank\"\u003eP05112\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eBSF1; IL-4; BCGF1; BSF-1; BCGF-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-4 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-4 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-4 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6 - 1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-4. The factors listed below were prepared at 100ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2 sRα, IL-3, IL-5, IL-6, IL-7, IL-8, LIF, TGF-β1, TGF-β2, TNF-α, TNF-β, SCF, VEGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-3, IL-5, IL-6, IL-7, IL-9, IL-10, IL-13, GM-CSF, LIF, MIP-1α, MIP-1β, TNF-α, SCF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ebovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-β1, porcine TGF-β1, rat TGF-β5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1002\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-2-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-2-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098478131,"sku":"BSKH1002","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1016","title":"Mouse IL-2 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-2 is added to the wells and binds to the combination of capture antibody-IL-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-2 standard dilutions and IL-2 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. Human IL-2 (also known as T-cell growth factor) is produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation.\u003cbr\u003e\u003cbr\u003eAdditionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes. IL-2 is involved in the treatment of cancers such as melanoma and renal cell cancer. It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types. \u003cbr\u003e\u003cbr\u003eThe sequence of mouse IL-2 cDNA predicts a 169 amino acid (aa) residue precursor glycoprotein containing a 20 aa residue signal peptide that is cleaved to form the mature protein. At the amino acid sequence level, mature mouse IL-2 is approximately 60% identical to human IL-2. Whereas human IL-2 is active on mouse cells, mouse IL-2 is species-specific and is inactive on human cells. The gene for IL-2 has been mapped to mouse chromosome.\u003cbr\u003e\u003cbr\u003eThe biological effects of IL-2 are mediated by specific cell surface receptor complexes. The functional high-affinity receptor for IL-2 is composed of three distinct polypeptide chains.\u003cbr\u003e\u003cbr\u003eIL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of natural killer cells and stimulates cytokine production by these cells as well as the cytolytic activity of these cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16183\" target=\"_blank\"\u003e16183\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P04351%20\" target=\"_blank\"\u003eP04351 \u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIl-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-2(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-2 Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-2 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-2. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-13, VEGF, LIF, MIP-1, MIP-2, TNF-α, SCF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-2, IL-2 sRα, IL-2sRβ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant porcine\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-2-elisa-kit.assay\" target=\"_blank\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-2-elisa-kit.assay\" target=\"_blank\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1016\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-2-elisa-kit.assay\" target=\"_blank\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-2-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098510899,"sku":"BSKM1016","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1068","title":"Human IL-33 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-33 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-33 is added to the wells and binds to the combination of capture antibody- IL-33 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-33 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-33 standard dilutions and IL-33 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a 30 kDa pro-inflammatory protein that plays an important role in Th2-biased immune responses and cardiac pathology. Human IL-33 is synthesized as a 270 amino acid (aa) molecule with an N-terminal nuclear localization signal, a helix-turn-helix motif, and a C-terminal region with structural homology to IL-1 family cytokines. Full length IL-33 interacts with nuclear chromatin, binds NFκB, and inhibits pro-inflammatory NFκB transactivation. Cleavage of full-length IL-33 leads to the extracellular release of an 18-20 kDa C-terminal fragment known as mature IL-33. Cathepsin G, Elastase, and Proteinase 3 can each cleave full length IL-33, giving rise to N-terminal heterogeneity of the mature form. IL-33 can be inactivated by further cleavage at several sites by Proteinase 3 and Caspase-1. Additional isoforms of human IL-33 with internal deletions are generated by alternative splicing. Mature human IL-33 shares 57% and 59% aa sequence identity with mouse and rat IL-33, respectively. \u003cbr\u003e\u003cbr\u003eIL-33 binds the transmembrane receptor ST2\/IL-1 R4 which subsequently associates with IL-1 RAcP to enable IL-33 dependent signaling. IL-1 RAcP is a shared signaling subunit that also associates with the receptors IL-1 RI, IL-1 RII, IL-1 R6, and SCF R\/c-kit. A soluble isoform of ST2 retains the ability to bind IL-33 and blocks ST2-dependent responses. Soluble IL-1 RAcP enhances the decoy function of soluble ST2. IL-33 binding to transmembrane ST2 induces the association of ST2 with existing IL-1 RAcP\/SCF R complexes. Activation of either ST2 or SCF R by their respective ligands can induce signal transduction through the other receptor subunit. IL-33 signaling through ST2 additionally triggers VE-Cadherin phosphorylation and internalization on vascular endothelial cells which leads to increased vascular permeability, vessel sprouting, and tubule formation.\u003cbr\u003e\u003cbr\u003eIL-33 exerts multiple effects on immune system function. It acts on Th2 cells, basophils, and mast cells to induce their migration to sites of inflammation and production of Th2 cytokines. IL-33 also promotes the expansion of regulatory T cells and alternately activated macrophages while attenuating Th17 cell expansion and activation. IL-33 contributes to infection clearance by enhancing neutrophil sensitization to TLR and Dectin-1 signaling, phagocytic activity, and migration to sites of infection. It is upregulated in a wide variety of cells under inflammatory conditions. Full length IL-33 is also found at elevated levels in bronchiolar lavage fluid during pulmonary fibrosis. \u003cbr\u003e\u003cbr\u003eThe full-length protein is classified as an alarmin due to its release from physically damaged or necrotic cells and its ability to trigger inflammatory and anti-viral CD8+ T cell responses. Like mature IL-33, the full-length protein activates ST2 and promotes mast cell activation and neutrophil infiltration. IL-33 induces both protective and pathologic actions in the heart. It counteracts cardiac myocyte hypertrophy and responsiveness to angiotensin II and phenylephrine. It is induced in cardiac fibroblasts by mechanical stress and circulates at elevated levels during chronic heart failure (as does the full-length form). The soluble ST2 receptor is elevated in the serum of heart failure as well as asthma patients. IL-33 inhibits the development of atherosclerotic plaques and induces the production of anti-oxidized LDL antibodies. It can also enhance eosinophilic perimyocarditis and impair heart function. In other settings, IL-33 limits neutrophil infiltration and circulating inflammatory chemokine levels following hepatic ischemia\/reperfusion injury but exacerbates CD4+ T cell infiltration and tissue damage following cisplatin-induced acute kidney injury.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/90865\" target=\"_blank\"\u003e90865\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/O95760\" target=\"_blank\"\u003eO95760\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eDVS27; IL1F11; NF-HEV; NFEHEV; C9orf26\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-33 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-33 Standard lyophilized, 1500 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-33 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 11pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e23.43 - 1500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-33. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-18, IL-1b F7, IL-1α, IL-1β, IL-18 Rα, IL-18 Rβ, IL-36 a, IL-36 β, IL-37, IL-38, ST2\/IL-1 R4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-36a, IL-1β, IL-36 β, IL-18\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-33-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-33-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1068\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-33-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-33-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098543667,"sku":"BSKH1068","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1004","title":"Human IL-4 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-2 is added to the wells and binds to the combination of capture antibody- IL-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-2 standard dilutions and IL-2 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. Human IL-2 (also known as T-cell growth factor) is produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation.\u003cbr\u003e\u003cbr\u003eIL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes. IL-2 is involved in the treatment of cancers such as melanoma and renal cell cancer. It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types. \u003cbr\u003e\u003cbr\u003eThe sequence of human IL-2 cDNA predicts a 153 amino acid (aa) residue precursor glycoprotein containing a 20 aa residue signal peptide that is cleaved to form the mature protein. At the amino acid sequence level, mouse IL-2 is approximately 60% identical to human IL-2. Whereas human IL-2 is active on mouse cells, mouse IL-2 is species-specific and is inactive on human cells. The gene for IL-2 has been mapped to human chromosome 4q.\u003cbr\u003e\u003cbr\u003eThe biological effects of IL-2 are mediated by specific cell surface receptor complexes. The functional high-affinity receptor for IL-2 is composed of three distinct polypeptide chains. \u003cbr\u003e\u003cbr\u003eIL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of natural killer cells and stimulates cytokine production by these cells as well as the cytolytic activity of these cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3558\" target=\"_blank\"\u003e3558\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P60568\" target=\"_blank\"\u003eP60568\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eInterleukin 2;IL-2;T Cell Growth Factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-2 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-2 Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-2 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.81- 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-2. The factors listed below were prepared at 100ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-2 sRα, IL-2 Rα, IL-2 Rγ, IL-3, IL-4, IL-6, IL-7, IL-8, LIF, TGF-β1, TGF-β2, TNF-α, TNF-β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-2, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ebovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-β1, porcine TGF-β1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-4-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-4-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1004\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-4-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-4-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098576435,"sku":"BSKH1004","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1003","title":"Mouse IL-4 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-4 is added to the wells and binds to the combination of capture antibody-IL-4 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-4 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-4 standard dilutions and IL-4 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils.\u003cbr\u003e\u003cbr\u003eThe cDNA sequence of mouse IL-4 predicts a 140 amino acid (aa) residue precursor protein containing a 20 aa residue signal peptide that is cleaved to form the mature protein. At the amino acid sequence level, mature mouse IL-4 is approximately 50% identical to human IL-4 but there is no species cross-reactivity for biological activity for the two proteins. Mouse IL-4 also shares approximately 30% amino acid sequence identity to mouse IL-13 and the two cytokines exhibit overlapping biological activities. The gene for IL-4 has been mapped to mouse chromosome 11, in close proximity to the genes for IL-3, IL-5, IL-13 and GM-CSF.\u003cbr\u003e\u003cbr\u003eIL-4 has multiple immune response-modulating activities on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro.\u003cbr\u003e\u003cbr\u003eThe biological effects of IL-4 are mediated by specific cell surface receptor complexes. Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13. In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates. Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16189\" target=\"_blank\"\u003e16189\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P07750\" target=\"_blank\"\u003eP07750\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-4; BSF-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-4(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-4 Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-4 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6 - 1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-4. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2, IL-3, IL-5, IL-6, IL-7, IL-9, IL-10, IL-13, LIF, VEGF, MIP-2, TNF-α, SCF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-4, IL-4sRα\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-4-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-4-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1003\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-4-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-4-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098609203,"sku":"BSKM1003","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1019","title":"Mouse IL-5 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-5 is added to the wells and binds to the combination of capture antibody-IL-5 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-5 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-5 standard dilutions and IL-5 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eMouse interleukin 5 (mIL-5), also known as T-cell replacing factor, B-cell growth factor II, eosinophil differentiation factor and eosinophil colony stimulating factor, is a pleiotropic cytokine produced primarily by T cells. It supports the proliferation and differentiation of mouse, but not human, B cells, and enhances IgM, IgG1, IgA, and IgE secretion. IL-5 chemo attracts and enhances the survival and the effector functions of mature eosinophils and synergizes with various colony stimulating factors to increase eosinophil progenitor production and eosinophil expansion. Because of its diverse effects on eosinophils, IL-5 is strongly implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions. Mouse IL-5 cDNA encodes a 133 amino acid (aa) residue precursor protein containing a hydrophobic signal peptide that is cleaved to yield a 113 aa residue mature protein. Native mouse IL-5 is a disulfide-linked homodimeric 40-45 kDa glycoprotein with N- and O-linked carbohydrate chains. Mature human IL-5 is approximately 70% identical at the amino acid level to mouse IL-5. Whereas mouse and human IL-5 are equally active on human cell lines, human IL-5 is much less active than mouse IL-5 in mouse cell assays. IL-5 is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16191\" target=\"_blank\"\u003e16191\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P04401\" target=\"_blank\"\u003eP04401\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eInterleukin-5, IL5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-5(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-5 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-5 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-5. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2 , IL-3, IL-4, IL-6, IL-7, IL-9, IL-10, IL-12, IL-17, LIF, Leptin, TNF-α, VEGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-5, IL-5sR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-5-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-5-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1019\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-5-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-5-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098641971,"sku":"BSKM1019","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1007","title":"Human IL-6 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-6 is added to the wells and binds to the combination of capture antibody- IL-6 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-6 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-6 standard dilutions and IL-6 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 6(IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte\/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6.\u003cbr\u003e\u003cbr\u003eThe human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa. The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence. On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene.\u003cbr\u003e\u003cbr\u003eThe effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion. IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression.\u003cbr\u003e\u003cbr\u003eIL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-γ. IL-6 stimulates production of acute phase proteins by hepatocytes and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma\/hybridoma\/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells.\u003c\/p\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3569\" target=\"_blank\"\u003e3569\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P05231\" target=\"_blank\"\u003eP05231\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-6 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-6 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-6 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 2pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e3.90 - 250 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-6. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCD40 Ligand, CT-1, CTLA-4, G-CSF, sgp130, IL-6 sR, IL-6 sR\/sgp130, IL-9, IL-10, IL-12, IL-12\/23 p40, LIF, LIF R, OSM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-6, IL-10, IL-11, IL-12, IL-12\/23 p40, IL-13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant rat\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCNTF, G-CSF, IL-6, OSM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1007\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-6-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098674739,"sku":"BSKH1007","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1004","title":"Mouse IL-6 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-6 is added to the wells and binds to the combination of capture antibody-IL-6 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-6 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-6 standard dilutions and IL-6 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis. It is expressed by a variety of normal and transformed lymphoid and nonlymphoid cells. \u003cbr\u003e\u003cbr\u003eIL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction. The mouse, rat and human IL-6 cDNAs have been cloned. The mouse IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide with a hydrophobic signal sequence that is cleaved to generate the 187 aa residue mature protein. Mouse to rat and human, there is approximately 87% and 39% aa identity, respectively. Although human and mouse IL-6 are equally active on mouse cells, mouse IL-6 is not active on human cells.\u003cbr\u003e\u003cbr\u003eThe high-affinity IL-6 receptor complex, which mediates IL-6 bioactivity, consists of two membrane glycoproteins. The production of IL-6 is upregulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/16193\" target=\"_blank\"\u003e16193\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P08505\" target=\"_blank\"\u003eP08505\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eInterleukin-6, IL6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-6(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IL-6 Standard lyophilized, 500 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse IL-6 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8-500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse IL-6. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-6, IL-6 sR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCT-1, gp130, IL-16 sR, IL-11\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant porcine\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1004\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-il-6-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098707507,"sku":"BSKM1004","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskr1005","title":"Rat IL-6 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-6 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any IL-6 present is bound by the immobilized antibody and the detection antibody specific for IL-6 is binds to the combination of capture antibody-IL-6 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-6 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-6 standard dilutions and IL-6 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis.\u003cbr\u003e\u003cbr\u003eIL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction. The rat, mouse and human IL-6 cDNAs have been cloned. Rat IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6.\u003cbr\u003e\u003cbr\u003eIL-6 is expressed by a variety of normal and transformed lymphoid and non-lymphoid cells. The production of IL-6 is up-regulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/24498\" target=\"_blank\"\u003e24498\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P20607\" target=\"_blank\"\u003eP20607\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eILg6; Ifnb2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with antibody to rat IL-6 (8-12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRat IL-6 Standard lyophilized, 4000pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003econcentrated Biotin-Conjugate anti-rat IL-6 antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 32pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e62.50 - 4000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant rat IL-6. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-6, IL-6sR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant rat\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-4, IL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKR1005\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-il-6-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-rat-il-6-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098740275,"sku":"BSKR1005","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1008","title":"Human IL-8 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-8 is added to the wells and binds to the combination of capture antibody- IL-8 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-8 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8 standard dilutions and IL-8 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eInterleukin 8 (IL-8), a member of the neutrophil-specific CXC subfamily of chemokines, is a potent neutrophil chemotactic and activating factor. It is a primary inflammatory cytokine produced by many cells including monocytes\/ macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes and chondrocytes. It is in response to proinflammatory stimuli such as IL-1, TNF, LPS and viruses. Its function is, in part, to attract neutrophils to the site of inflammation and to activate them.\u003cbr\u003e\u003cbr\u003eThe human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa). Further proteolysis of the N-terminal end leads to a variant form with 72 amino acids; full activation of IL-8 may require cleavage to the 72 amino acid form.\u003cbr\u003e\u003cbr\u003eIL-8 can form non-covalent dimers in solution, especially at high concentrations, but dimerization is not necessary for biological activity. IL-8 binds to two seven-transmembrane, G protein-coupled receptors, CXCR1 and CXCR2, as well as to the non-signalling Duffy antigen on red-blood cells. The Duffy antigen may play a role in regulating IL-8 activity on functional receptors.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3576\" target=\"_blank\"\u003e3576\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P10145\" target=\"_blank\"\u003eP10145\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCXCL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-8 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-8 Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human IL-8 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6 - 1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human IL-8. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eENA-78, BLC\/BCA-1, GCP-2, GROα, GROβ, GROγ, IP-10, I-TAC, MIG, NAP-2, PF4, SDF-1α, SDF-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eBLC\/BCA-1, GCP-2, CRG-2, KC, MIG, SDF-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-8-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-8-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1008\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-il-8-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-il-8-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098773043,"sku":"BSKH1008","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1027","title":"Human Leptin ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Leptin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Leptin present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Leptin is added to the wells and binds to the combination of capture antibody- Leptin in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of Leptin present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Leptin standard dilutions and Leptin sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eHuman Leptin (gene name OB) is a 16 kDa, 146 amino acid (aa) residue, non-glycosylated polypeptide that regulates adipose tissue mass and energy balance. Mature human Leptin shares 87% and 84% aa identity with mouse and rat Leptin, respectively. Human Leptin is active in both the mouse and rat systems. Leptin is expressed almost exclusively by adipocytes and its production is influenced by hormones, cytokines and nutrients and circulates in the plasma, crosses the blood-brain barrier, and is present in human breast milk. The human Leptin receptor (designated ObR or LEPR) is a 150 kDa, 1144 aa residue, type I transmembrane glycoprotein of the IL-6 receptor family of Class I cytokine receptors. The gene for ObR undergoes considerable splicing, forming variants a-d with cytoplasmic domains of variable length, plus the potentially soluble form ObRe. The long form, ObRb (formerly OB RL), is expressed mainly in the hypothalamic arcuate nucleus and is essential for signal transduction. In a concentration-dependent manner, Leptin signaling can have diverse effects, causing neurons that express pro-opiomelanocortin (POMC) peptides to reduce food intake, and neurons that express neuropeptide Y and agouti-related protein (NpY and AgRP) to increase food intake. Leptin is fundamentally a “starvation signal” that, when low, prompts increased appetite and decreased energy expenditure. Leptin deficiency influences the immune system, depressing Th1 responses and causing increased frequency of infections. Leptin also regulates puberty, blocking the onset of puberty, or of menses if Leptin deficiency exists due to excessive thinness, such as results from starvation, extreme exercise-induced weight loss, anorexia or cancer-induced cachexia.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3952\" target=\"_blank\"\u003e3952\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P41159\" target=\"_blank\"\u003eP41159\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eOB; OBS; LEPD\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human Leptin (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Leptin Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human Leptin monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 bottles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 15pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e31.25 - 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human Leptin. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12, TGF-β1, TGF-β3, TNF-α, TNF-β, IFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-13, Leptin, LIF, MIP-1α, MIP-1β, TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ebovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-β1, porcine TGF-β1, porcine TGF-β2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-leptin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-leptin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1027\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-leptin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-leptin-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098805811,"sku":"BSKH1027","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1048","title":"Human lipocalin-2\/NGAL ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Lipocalin-2\/NGAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lipocalin-2\/NGAL present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Lipocalin-2\/NGAL is added to the wells and binds to the combination of capture antibody-Lipocalin-2\/NGAL in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of Lipocalin-2\/NGAL present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Lipocalin-2\/NGAL standard dilutions and Lipocalin-2\/NGAL sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eMembers of the Lipocalin family have limited sequence identity, but share a highly conserved fold with an eight-stranded anti-parallel β barrel motif that encloses an internal ligand-binding site. They are known for their actions as transporters that carry small hydrophobic molecules such as steroid hormones, vitamins, odorants, and metabolic products. Lipocalin-2, also known as Neutrophil Gelatinase-associated Lipocalin (NGAL) or Siderocalin, was originally identified as a component of neutrophil granules. Since then, its expression has been observed in most tissues, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of cellular processes including the innate immune response, differentiation, tumorigenesis, and cell survival. It is a 25 kDa protein existing in monomeric, homodimeric, and heterodimeric forms, the latter in association with human matrix metalloproteinase 9 (MMP-9). Its association with MMP-9 may modulate protease activity by protecting MMP-9 from degradation. The mouse ortholog (also known as 24p3) shares 62% sequence identity at the amino acid level.\u003cbr\u003e\u003cbr\u003eThe functions of Lipocalin-2 continue to be elucidated. Studies indicate that it binds bacterial catecholate siderophores bound to ferric ions. This suggests that Lipocalin-2 may act as a bacteriostatic agent by binding bacterial siderophores and limiting bacterial iron supply. This is supported by the observation that mouse Lipocalin-2 is induced in immune cells following Toll-like receptor activation, and Lipocalin-2 mouse knockouts exhibit decreased ability to counter bacterial infection. Lipocalin-2 may also regulate iron uptake into mammalian cells. In the kidney, Lipocalin-2-mediated iron trafficking may be involved in both development and protection from renal injury. Megalin, a member of the LDL receptor family, and 24p3 R have been reported as endocytic receptors for Lipocalin-2. It should be noted that the effects of Lipocalin-2 on cells might be context-dependent. For instance, it has been shown to act as both a survival factor and a pro-apoptotic factor, and its induction by pro-inflammatory cytokines may vary between mouse and human.\u003cbr\u003e\u003cbr\u003eLipocalin-2 has been associated with several pathological processes. For instance, it is upregulated in psoriatic skin in comparison to uninvolved control skin, and Lipocalin-2 suppresses red blood cell production in models of anemia. Lipocalin-2 is elevated in patients with severe acute respiratory syndrome (SARS), and may act as a biomarker for acute renal injury. It has been associated with several tumor types as well, including breast, ovarian, colorectal, and pancreatic cancers. Its function in cancer is unclear, although the invasive and metastatic behavior of tumor cells is suppressed by Lipocalin-2 in models of breast and colon cancer.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/3934\" target=\"_blank\"\u003e3934\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P80188%20\" target=\"_blank\"\u003eP80188 \u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ep25; 24p3; MSFI; NGAL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human Lipocalin-2\/NGAL (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Lipocalin-2\/NGAL Standard lyophilized, 2500 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human Lipocalin-2\/NGAL monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 bottles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 40pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e78 - 5000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human Lipocalin-2\/NGAL. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCOX-2, Lipocalin-1, MMP-9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eLipocalin-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-lipocalin-2-ngal-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-lipocalin-2-ngal-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1048\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-lipocalin-2-ngal-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-lipocalin-2-ngal-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098838579,"sku":"BSKH1048","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1040","title":"Human MCP-1 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MCP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MCP-1 is added to the wells and binds to the combination of capture antibody- MCP-1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of MCP-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MCP-1 standard dilutions and MCP-1 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eMonocyte chemoattractant protein-1 (MCP-1) called also monocyte chemotactic protein-1. MCP-1 is an 8.6kDa protein and belongs to the family of chemotactic cytokines known as Chemokines. MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, osteoblastic cells, and human pulmonary type-2-like epithelial cells in culture. MCP-1 exhibits chemotactic activity for monocytes\/macrophages, basophils, T lymphocytes, particularly memory T cells and natural killer cells and neural stem cells. Elevated levels of MCP-1 are detected during inflammation and immune responses. MCP-1 activity is also important in wound healing as shown by delayed wound repair in MCP-1deficient mice. MCP-1 is implicated in the pathogenesis of disease states that involve monocyte\/macrophage infiltrates, such as psoriasis, rheumatoid arthritis and recruitment of monocytes to the arterial wall in atherosclerosis. In addition to its chemotactic function, MCP-1 also induces the expression of IL-10 from macrophages, which favours a TH2 immune response. MCP-1 is a glycopeptide containing one potential N-linked glycosylation site. The extent of MCP-1 glycosylation influences its chemotactic potency and half-life in vivo.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/6347\" target=\"_blank\"\u003e6347\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P13500%20\" target=\"_blank\"\u003eP13500 \u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eHC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human MCP-1 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MCP-1 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human MCP-1 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003e: The minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.60 -1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human MCP-1. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eGROα, GROβ, GROγ, IL-8, MCP-2, MCP-3, MIP-1α, MIP-1β, RANTES\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eJE\/MCP-1, MIP-1α, MIP-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-mcp-1-elis-akit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-mcp-1-elis-akit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1040\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-mcp-1-elis-akit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-mcp-1-elis-akit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098871347,"sku":"BSKH1040","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1011","title":"Mouse MCP-1 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MCP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MCP-1 is added to the wells and binds to the combination of capture antibody-MCP-1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of MCP-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MCP-1 standard dilutions and MCP-1 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eThe mouse JE gene was originally described as a platelet-derived growth factor-inducible gene in mouse fibroblasts. The protein encoded by mouse JE was found to belong to the large CC chemokine family of inflammatory and immunoregulatory cytokines. Among CC chemokine family members, JE is functionally and structurally most closely related to the MCP\/eotaxin subfamily of proteins. Within this MCP\/eotaxin subfamily, five human (MCP-1, 2, 3, 4 and eotaxin) and four mouse (JE\/MCP-1, MARC\/MCP-3, MCP-5, and eotaxin) proteins have been identified. At the amino acid (aa) sequence level, mature human MCP-1 shows 55%, 59%, and 66% identity with the analogous regions of mouse JE, MARC, and MCP-5, respectively. Although JE has been presumed to be the mouse homolog of human MCP-1, the more recently isolated mouse MCP-5 is actually more homologous and may be considered to be a second human MCP-1 homolog.\u003cbr\u003e\u003cbr\u003eMouse MCP-1 cDNA encodes a 148 aa residue precursor protein with a predicted 23 aa residue signal peptide that is cleaved to generate a putative mature protein of 125 aa residues. Compared to mature human MCP-1, mouse MCP-1 has a 49 aa residue carboxy-terminal extension that is rich in serine and threonine residues. Recombinant MCP-1 expressed in CHO cells as well as natural MCP-1 purified from mouse astrocytes and a mouse thymic epithelial cell line, were shown to be approximately 30 kDa glycoproteins with multiple O-linked oligosaccharide chains added to the 49 aa residue C-terminal domain. Nonetheless, the natural form of MCP-1 produced by virus-stimulated mouse L929 fibroblasts occurs as a non-glycosylated 7-8 kDa protein that lacks the C-terminal domain. The carboxy-terminal domain has been found not to be required for mouse MCP-1 activity. Besides fibroblasts, astrocytes and epithelial cells, mouse MCP-1 has been found to be expressed in macrophages, mast cells, endothelial cells, osteoblasts and ameloblasts. The expression of mouse MCP-1 is induced after stimulation with inflammatory stimuli including viruses, LPS, and cytokines such as TNF-α, IL-1, IFN-γ, and PDGF.\u003cbr\u003e\u003cbr\u003eMouse MCP-1 is a potent chemoattractant for monocytes\/macrophages and lymphocytes. It has also been shown to be involved in the regulation of Th1\/Th2 lymphocyte differentiation, enhancing Th2 development by increasing IL-4 production and inhibiting IL-12 production. The activities of mouse MCP-1 have been shown to be mediated by the mouse CC chemokine receptor CCR2, a G protein-coupled, seven transmembrane domain receptors. Mouse CCR2 cDNA encodes a 373 aa residue protein that shows the highest (80%) overall identity at the aa sequence level with the human MCP-1 receptor, CCR2B. The gene for mouse CCR2 has been mapped to mouse chromosome 9, in close proximity with mouse CCR1 and CCR3. High levels of mouse CCR2 expression have been detected in monocytes\/macrophages.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/20296\" target=\"_blank\"\u003e20296\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P10148\" target=\"_blank\"\u003eP10148\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eJE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse MCP-1(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse MCP-1 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse MCP-1 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse MCP-1. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, GM-CSF, M-CSF, SCF, TNF-α, IFN-r, VEGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-2 CCL2\/MCP-1, CCL3\/MIP-1α, CCL4\/MIP-1β, CCL5\/RANTES, CCL7\/MCP-3, CCL7\/MCP-3, CCL11\/Eotaxin, CXCL1\/GROα, CXCL1\/GROα, CXCL2\/GROβ, CXCL8\/IL-8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-mcp-1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-mcp-1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1011\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-mcp-1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-mcp-1-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098904115,"sku":"BSKM1011","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1042","title":"Human MMP-9 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MMP-9 is added to the wells and binds to the combination of capture antibody- MMP-9 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of MMP-9 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MMP-9 standard dilutions and MMP-9 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eMatrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade extracellular matrix proteins. They are secreted as zymogens (Pro-MMPs) that are activated by a variety of proteinases or by reaction with organic mercurials. They are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs) and by 2-macroglobulin. The regulation of MMP activity is important in tissue remodeling, inflammation, tumor growth and metastasis.\u003cbr\u003e\u003cbr\u003eHuman MMP-9 (also known as gelatinase B) is secreted as a 92 kDa zymogen. Cleavage of Pro-MMP-9 at or near residue 87 results in the active enzyme with a mass of approximately 82 kDa. MMP-9 has three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain. Pro-MMP-9 can be activated by MMP-3 or by certain bacterial proteinases. MMP-9 is inhibited by α2-macroglobulin or by TIMP-1, which binds to Pro-MMP-9 as well as to active MMP-9. In vitro treatment of Pro-MMP-9 with 4-aminophenylmercuric acid (APMA) produces not only the 82 kDa active enzyme but also a C-terminal truncated form of approximately 65 kDa with the activity comparable to that of the 82 kDa form.\u003cbr\u003e\u003cbr\u003ePro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells. Pro-MMP-9 expression is upregulated by TGF-β1, IL-1β, TGF-α, PDGF-AB, TNF-α, and IL-1α. Substrates for MMP-9 include denatured collagen type I (gelatin), native collagens type IV, V, VII, X and XI, fibrinogen, vitronectin, IL-1β, and entactin, a molecule that bridges laminin and type IV collagen.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/4318\" target=\"_blank\"\u003e4318\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P14780\" target=\"_blank\"\u003eP14780\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eGELB; CLG4B; MMP-9; MANDP2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human MMP-9 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman MMP-9 Standard lyophilized, 4000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human MMP-9 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 30pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e62.50 - 4000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human MMP-9. The factors listed below were prepared at 200ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eMMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-10, MMP-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eMMP-2, MMP-3, MMP-9, TIMP-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-mmp-9-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-mmp-9-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1042\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-mmp-9-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-mmp-9-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098936883,"sku":"BSKH1042","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1069","title":"Human Resistin ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Resistin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Resistin present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Resistin is added to the wells and binds to the combination of capture antibody- Resistin in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of Resistin present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Resistin standard dilutions and Resistin sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eResistin, also known as Found In Inflammatory Zone 3 (FIZZ3) or Adipocyte Secreted Factor (ADSF), is a member of a protein family known as the Resistin-like molecules (RELMs). It is perhaps best known for its potential as a link between obesity and the development of insulin resistance. Other members of this family include RELM-α\/FIZZ1 and RELM-γ, which are described in rodents but as yet have no identified human counterparts, and RELM-β\/FIZZ2. The Resistin amino acid (aa) sequence contains a putative N-terminal signal sequence and a motif containing 11 cysteine residues, 10 of which are characteristic of the RELM family. The protein is thought to be secreted as a dimer and potentially exists in higher order molecular structures resulting from interactions between Resistin dimers or other members of the RELM family. A splice variant in the rat, lacking the signal sequence and localized predominantly to the nucleus, has also been described. A large 3' intron is the primary reason that the mouse genomic sequence is 3-fold larger than the corresponding human sequence. Mouse and human Resistin share only 53 percent identity at the aa level and exhibit differences in expression patterns. In mouse, expression appears primarily in adipose tissues. Although some human studies suggest Resistin is expressed by adipose tissues as well, the most significant source appears to be blood mononuclear cells. In humans, Resistin is also reported to be expressed by pre-adipocytes, placenta, pancreatic islets, and primary leukemia cells. A receptor for Resistin has not yet been described. \u003cbr\u003e\u003cbr\u003eResistin acquired initial attention as a potential link between obesity and glucose regulation. Serum levels were shown to increase in diet-induced and genetic forms of obesity in mice (ob\/ob and db\/db) and decrease in response to insulin sensitizing drugs (TZDs). In addition, function-blocking Resistin antibodies enhanced insulin actions while treatment with recombinant Resistin caused glucose intolerance and insulin resistance. Resistin knockout mice exhibit decreased fasting blood glucose levels as a result of reduced hepatic output. To establish a physiological role in humans, several studies have examined whether altered circulating Resistin levels are associated with type 2 diabetes, insulin resistance, and\/or obesity. Although some demonstrate significant correlations, others report no correlation, suggesting that in humans fundamental questions remain regarding Resistin’s role in these pathophysiological processes. Resistin expression by human mononuclear cells could indicate a potential role in inflammation. In vitro, Resistin expression by these cells is enhanced by treatment with several pro-inflammatory cytokines including IL-1β, TNF-α, IFN-γ, or IL-6. In addition, Resistin has been shown to activate endothelial cells in vitro, leading to the production of adhesion molecules, Endothelin-1, and chemokines.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/56729\" target=\"_blank\"\u003e56729\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q9HD89\" target=\"_blank\"\u003eQ9HD89 \u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eADSF; RSTN; XCP1; FIZZ3; RETN1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human Resistin (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Resistin Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human Resistin monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 bottles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8 - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human Resistin. The factors listed below were prepared at 20ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1β, IL-2 sRα\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-3, IL-5, IL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-resistin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-resistin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1069\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-resistin-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-resistin-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740098969651,"sku":"BSKH1069","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1066","title":"Human S100B ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for S100B has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any S100B present is bound by the immobilized antibody and the detection antibody specific for S100B is binds to the combination of capture antibody-S100B in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of S100B present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven S100B standard dilutions and S100B sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eS100B belongs to the S100 subgroup of the EF-hand family of calcium binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/6285\" target=\"_blank\"\u003e6285\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P04271\" target=\"_blank\"\u003eP04271\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eNEF; S100; S100-B; S100beta\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with antibody to human S100B (812)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman S100B standard lyophilized, 6000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human S100B antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 46pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e93.75 - 6000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human S100B. The factors listed below were prepared at 50ng\/ml in standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eNDR1, p53, RAGE\/Fc Chimera, S100A6, S100A4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-s100b-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-s100b-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1066\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-s100b-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-s100b-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099002419,"sku":"BSKH1066","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1021","title":"Human TGF-β1 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TGF-β1 is added to the wells and binds to the combination of capture antibody- TGF-β1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TGF-β1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TGF-β1 standard dilutions and TGF-β1 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eTransforming growth factor (TGF), a ‘factor’ that promoted the transformation of cultured fibroblasts into a tumor-like phenotype, was subsequently found to be more of a tumor suppressor than tumor promoter and to be a mixture of two proteins, TGF-α and TGF-β. These molecules are members of a superfamily that includes TGF-β1 through 5, bone morphogenic proteins, activins and inhibins. It plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis.\u003cbr\u003e\u003cbr\u003eOriginally, TGF-β1 was separated from platelets and later found that TGF-β1 can be expressed in many organizations. Human TGF-β1 is a 25kDa, disulfide-linked, non-glycosylated homodimer. Biological activity of TGF-β1 is regulated by a number of receptors, including receptor I (53-65KD), receptor II (83-110KD), receptor III (250-310KD), receptor-IV (60KD) and receptor V (400KD).\u003cbr\u003e\u003cbr\u003eTGF-β1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF-β1 exhibits important immunoregulatory features of partially adverse character: TGF-β1 inhibits B and T cell proliferation, differentiation and antibody production as well as maturation and activation of macrophages. TGF-β1 is synthesized, with only a few exceptions, by virtually all cells, and TGF receptors are expressed by all cells. TGF-β affects nearly every physiological process in some way; its systemic and cell-specific activities are too complicated to review here. There are, however, three fundamental activities: TGF-β1 modulates cell proliferation, generally as a suppressor; TGF-β1 enhances the deposition of extracellular matrix through promotion of synthesis and inhibition of degradation; TGF-β1 is immunosuppressive through a variety of mechanisms. The specific action of TGF-β on a particular cell depends on the exact circumstances of that cell’s environment.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/7040\" target=\"_blank\"\u003e7040\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P01137\" target=\"_blank\"\u003eP01137\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human TGF-β1 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman TGF-β1 Standard lyophilized, 4000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human TGF-β1 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 bottles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 31pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e62.5 - 4000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human TGF-β1. The factors listed below were prepared at 100ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eActivin A, Activin RIA, Activin RIIA, Activin RIIB, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-8b, BMP-10, BMP-15, BMP-2, TGF-β2, TGF-β3, TGF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eBMP-3b, BMPR-IA , BMPR-IB, TGF-β RI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eNatural porcine TGF-β2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tgf-%ce%b21-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tgf-%ce%b21-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1021\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tgf-%ce%b21-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-tgf-%ce%b21-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099035187,"sku":"BSKH1021","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1008","title":"Mouse TGF-β1 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TGF-β1 is added to the wells and binds to the combination of capture antibody- TGF-β1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TGF-β1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TGF-β1 standard dilutions and TGF-β1 sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eTransforming Growth Factor-beta (TGF-β) is a pleiotropic cytokine which exists in five isoforms, known as TGF-β1-5. TGF-β1 is the most abundant form in lymphoid organs and is found almost ubiquitously while other isoforms are expressed in a more restricted distribution. The biologically active forms of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. TGF-β1 is produced in very high levels by platelets. Other cellular sources of TGF-β1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-β1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-β1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-β1 inhibits the proliferation of T cells and NK cells and down-regulates the activities of activated macrophages. TGF-β1 blocks the anti-tumor activity of IL-2–bearing lymphokine-activated killer (LAK) cells. TGF-β1 has a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-β1 and stimulate expansion of naturally-occurring T reg cells. Moreover, TGF-β1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-β-induced regulatory T cells have been termed Ti-Treg.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/21803\" target=\"_blank\"\u003e21803\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P04202\" target=\"_blank\"\u003eP04202\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTGF-beta1, TGFbeta1, Tgfb, Tgfb-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse TGF-β1 (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse TGF-β1 Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse TGF-β1 monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 bottles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1N HCl\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1 N NaOH\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2.7N NaOH\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2.5N Acetic Acid\/10 M Urea\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse TGF-β1. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTGF-β2, TGF-β3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTGF-β RI\/Fc Chimera\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-tgf-%ce%b21-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-tgf-%ce%b21-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1008\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-tgf-%ce%b21-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-tgf-%ce%b21-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099067955,"sku":"BSKM1008","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1070","title":"Human TIM-1\/KIM-1 ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TIM-1\/KIM-1\/HAVCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIM-1\/KIM-1\/HAVCR present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TIM-1\/KIM-1\/HAVCR is added to the wells and binds to the combination of capture antibody-TIM-1\/KIM-1\/HAVCR in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TIM-1\/KIM-1\/HAVCR present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TIM-1\/KIM-1\/HAVCR standard dilutions and TIM-1\/KIM-1\/HAVCR sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eT cell immunoglobulin and mucin domain 1 (TIM-1), also known as Kidney injury molecule 1 (KIM-1) and Hepatitis A virus cellular receptor 1 (HAVcr1), is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses. TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked glycosylations, a transmembrane segment, and a cytoplasmic signaling domain. Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain. Some of these polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A viral infection in humans. Within the extracellular domain, human TIM-1 shares 41% amino acid sequence identity with mouse and rat TIM-1. \u003cbr\u003e\u003cbr\u003eIn vivo, TIM-1 is expressed on splenic B cells and is a marker for the identification of IL-10+ regulatory B cells. TIM-1 is also expressed on CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium. The expression of TIM-1 is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury. Metalloproteinase-mediated cleavage of TIM-1 at the membrane-proximal region results in the release of a soluble form of TIM-1 which is detectable in the urine and in circulation. Urinary TIM-1 is highly elevated in nephropathy and may be a useful biomarker for renal damage.\u003cbr\u003e\u003cbr\u003eTIM-1 has been reported to be a receptor for a number of ligands, including phosphatidylserine, leukocyte mono-immunoglobulin-like receptor 5 (LMIR5\/CD300b), TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with phosphatidylserine enables TIM-1 to mediate the phagocytosis of apoptotic cells. In TIM-1-bearing iNKT cells, interaction with apoptotic cells can also result in iNKT cell activation, proliferation, and cytokine production. Interactions between cell-surface or soluble TIM-1 with LMIR5 is proposed to induce LMIR5-mediated activation of myeloid cells including macrophages\/monocytes, mast cells, neutrophils, and dendritic cells. These interactions contribute to tissue homeostasis and damage during kidney injury. Ligandinduced TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. In humans, TIM-1 serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus and Marburgvirus.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/26762\" target=\"_blank\"\u003e26762\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q96D42\" target=\"_blank\"\u003eQ96D42\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTIM; KIM1; TIM1; CD365; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human TIM-1\/KIM-1\/HAVCR (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman TIM-1\/KIM-1\/HAVCR Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human TIM-1\/KIM-1\/HAVCR monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6 - 1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human TIM-1\/KIM-1\/HAVCR. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTIM-3, TIM-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTIM-1, TIM-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant rat\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTIM-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tim-1-kim-1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tim-1-kim-1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1070\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tim-1-kim-1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-tim-1-kim-1-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099133491,"sku":"BSKH1070","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1014","title":"Human TNF-α ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TNF-α is added to the wells and binds to the combination of capture antibody- TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TNF-α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-α standard dilutions and TNF-α sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eThe prototype ligand of the TNF superfamily, TNF-α\/TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis. Human cells known to express TNF-α include B cells, colonic columnar epithelial cells, NK and CD3+CD56+ hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4+ and CD8+ T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.\u003cbr\u003e\u003cbr\u003eIt is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids (aa) in length. It contains a 30 aa cytoplasmic domain, a 26 amino acids transmembrane segment, and a 177 amino acids extracellular region. TNF-α is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNF-α (sTNF-α is released from the C-terminus of the transmembrane protein through the activity of TNF-α-converting enzyme (TACE), a membrane-bound disintegrin metalloproteinase). \u003cbr\u003e\u003cbr\u003eTNF-α is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms. Deficiencies in either TNF-α or its receptors can increase susceptibility to infection by intracellular pathogens. TNF- may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers. Other potential physiological roles for TNF-α and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells.\u003cbr\u003e\u003cbr\u003eTNF-α has been implicated in a number of pathophysiological processes. It is associated with unregulated pro-inflammatory activity and is thought to be a critical mediator of endotoxin-induced septic shock. Cachexia (or whole body wasting) has also been associated with long-term circulating TNF-α. Other disorders with potential TNF-α involvement include asthma), type 2 diabetes, Crohn’s disease, and rheumatoid arthritis.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/7124\" target=\"_blank\"\u003e7124\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P01375\" target=\"_blank\"\u003eP01375\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eDIF-alpha, TNFA, TNFSF2, TNLG1F, TNF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human TNF-α(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman TNF-α Standard lyophilized, 4000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human TNF-αmonoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6 - 1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human TNF-α. The factors listed below were prepared at 70ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-β, GM-CSF, s TNF RI, s TNF RII\/Fc, s TNF RII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-α, TNF-α（truncayed）, s TNF RI, s TNF RII IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant rat\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1014\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-tnf-%ce%b1-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099166259,"sku":"BSKH1014","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1002","title":"Mouse TNF-α ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TNF-α is added to the wells and binds to the combination of capture antibody-TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TNF-α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-α standard dilutions and TNF-α sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eThe prototype ligand of the TNF superfamily, TNF-α\/TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis. TNF-α is produced by activated macrophages and other cell types including T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells and some tumor cells.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003eMouse TNF-α cDNA encodes a 235 amino acid (aa) residue type II membrane protein. The 156 aa residue soluble TNF-α is released from the C-terminus of the membrane-anchored TNF-α by proteolytic cleavage by a matrix metalloprotease. \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003eThe membrane-anchored form of TNF-α has been shown to have lytic activity and may also have an important role in intercellular communication. The biologically active TNF-α has been shown to exist as a trimer. TNF-α is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms. Deficiencies in either TNF-α or its receptors can increase susceptibility to infection by intracellular pathogens. TNF-α may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers. Other potential physiological roles for TNF-α and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/21926\" target=\"_blank\"\u003e21926\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P06804\" target=\"_blank\"\u003eP06804\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eDIF, TNF-a, TNF-alpha, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse TNF-α(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse TNF-α Standard lyophilized, 1000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse TNF-α monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 3.9pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8 - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse TNF-α. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, C10, IFN-γ, IL-1α, IL-1β, IL-2 , IL-3, IL-5, IL-6, IL-7, IL-9, LIF, M-CSF, SCF, VEGF, TNF-β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-α, TNF sRI, TNF sRII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1002\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-tnf-%ce%b1-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099199027,"sku":"BSKM1002","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskw1007","title":"Swine TNF-α ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any TNF-α present is bound by the immobilized antibody and the detection antibody specific for TNF-α is binds to the combination of capture antibody-TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TNF-α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-α standard dilutions and TNF-α sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eTumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.\u003cbr\u003e\u003cbr\u003eCleavage of membrane bound TNF-α by TACE\/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/397086\" target=\"_blank\"\u003e397086\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P23563\" target=\"_blank\"\u003eP23563\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNFa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with antibody to Swine TNF-α (8-12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSwine TNF-α standard lyophilized, 2000pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-Swine TNF-α antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 15pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e31.25 - 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant Swine TNF-α. The factors listed below were prepared at 70ng\/ml in standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-β, GM-CSF, s TNF RI, s TNF RII, s TNF RII\/Fc\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-α（truncated, s TNF RI, s TNF RII IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-swine-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-swine-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKW1007\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-swine-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-swine-tnf-%ce%b1-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099231795,"sku":"BSKW1007","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskr1003","title":"Rat TNF-α ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-α has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps, any TNF-α present is bound by the immobilized antibody and the detection antibody specific for TNF-α is binds to the combination of capture antibody-TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TNF-α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-α standard dilutions and TNF-α sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg alt=\"\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eTumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. TNF-α is a mononuclear factor produced primarily by monocytes and macrophages. In 1975, Carswell et al. found that BCG attacked mice and then treated with endotoxin. A substance in the serum of mice that induced hemorrhagic necrosis of tumor tissue was named as tumor necrosis factor. In 1985, Shalaby named TNF produced by macrophages as TNF-α, and the lymphotoxin produced by T lymphocytes was named TNF-β.\u003cbr\u003e\u003cbr\u003eRat TNF-α is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 156 aa extracellular domain (ECD) (12). Within the ECD, rat TNF-α shares 95% aa sequence identity with mouse, and 73% - 79% aa identity with bovine, canine, cotton rat, equine, feline, human, rhesus macaque and porcine TNF-α. It is produced by a wide variety of immune, epithelial, endothelial and tumor cells. \u003cbr\u003e\u003cbr\u003eThe biological activities of TNF-α are very complex, including regulation of hematopoiesis, immunity and inflammation; effects on blood vessels and blood clotting. It also has the effects on various organs (liver, heart, bone, cartilage, muscle and other tissues). TNF-α regard as the growth factor for certain tumor cells. It has the synergy effect EGF, PDGF and insulin to promote the expression of EGF receptor. Recently, TNF-β (LT) has been reported to be an autocrine growth factor for Epstein-Barr virus-transformed lymphoblasts, and anti-LT antibodies, sTNF R. It also can inhibit the proliferation of Epstein-Barr virus-transformed lymphocytes.\u003cbr\u003e\u003cbr\u003eThe anti-tumor effects of TNF-α include the direct action of TNF-α and immune response that induced by TNF-α against tumors. TNF-α is involved in diseases including asthma, type II diabetes, Crohn's disease and rheumatoid arthritis. TNF stimulates endothelial cells that can cause inflammation, tissue damage and blood clotting to induce septic shock.\u003c\/p\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/24835\" target=\"_blank\"\u003e24835\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P16599\" target=\"_blank\"\u003eP16599\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eRATTNF, TNF-alphaa, Tnf\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with antibody to rat TNF-α (8-12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRat TNF-α Standard lyophilized, 2000pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003econcentrated Biotin-Conjugate anti-rat TNF-α antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 15pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e31.25 - 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant rat TNF-α. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant rat\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eCINC-1, GDNF, β-NGF, PDGF-BB, IFN-γ, IL-1β, IL-2, IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF-α, TNF-β, TNF sRI, TNF sRII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTNF sRI, TNF sRII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKR1003\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-rat-tnf-%ce%b1-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-rat-tnf-%ce%b1-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099264563,"sku":"BSKR1003","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1031","title":"Human TSLP ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TSLP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSLP present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TSLP is added to the wells and binds to the combination of capture antibody- TSLP in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TSLP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TSLP standard dilutions and TSLP sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eThymic Stromal Lymphopoietin (TSLP) is a 23 kDa member of the IL-7 family of a-helical cytokines. It is a monomeric glycoprotein that is synthesized as a 159 amino acid (aa) precursor. TSLP appears to have species-specific functions. In mouse, TSLP was initially reported to act on NK cells, mast cells, and B cells, but this does not appear to occur in humans. In humans, TSLP is produced by a number of divergent cell types, all of which appear to target T cells, monocytes, and\/or dendritic cells. On TCR-activated T cells, TSLP directly induces T cell proliferation. The significance of this direct action is unclear. On monocytes, TSLP is reported to induce the release of multiple chemokines that target CCR4, a receptor associated with the Th2 subset. TSLP is best known for its direct action on subsets of dendritic cells. In thymic medulla, Hassell’s corpuscle epithelium produces TSLP that acts on resident CD11c+ dendritic cells. This induces the expression of B7 family molecules on dendritic cells, which subsequently convert regional CD4+CD25+ (potentially) autoreactive T cells into CD4+CD25+FOXP3+ regulatory T cells. Allergen-challenged keratinocytes are also known to produce TSLP in skin where TSLP acts on Langerhans cells (CD1a+ immature dendritic cells) which then migrate to regional lymph nodes and express B7-2\/CD86, CD83, high levels of MHC-II, and TARC. TARC attracts naive CD4+ T cells to the TSLP-activated Langerhans cells, and this interaction induces a Th2 phenotype. TSLP-induced Th2 cells are strong producers of IL-13, IL-5, and TNF-a, all of which promote allergic-type inflammations.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/85480\" target=\"_blank\"\u003e85480\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q969D9\" target=\"_blank\"\u003eQ969D9\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eTSLP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human TSLP (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman TSLP Standard lyophilized, 500 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human TSLP monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 4pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e7.8 - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human TSLP. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12, IL-17, IL-22, LIF, Leptin, TNF-α, TNF-β, IFN-γ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-13, LIF, TSLP, TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant rat\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eIL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, Leptin, TNF-α\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tslp-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tslp-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1031\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-tslp-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-tslp-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099297331,"sku":"BSKH1031","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskh1024","title":"Human VEGF ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for VEGF is added to the wells and binds to the combination of capture antibody- VEGF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of VEGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven VEGF standard dilutions and VEGF sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eVascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is well known for its important roles in regulating both physiological and pathological blood vessel growth. It is a member of the VEGF family that also includes VEGF-B, -C, -D, -E, and PlGF (placental growth factor). Family members exhibit a cysteine knot motif formed by eight characteristically spaced cysteine residues.\u003cbr\u003e\u003cbr\u003eVarious cells and tissues express VEGF including skeletal and cardiac muscle, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, platelets, brown adipose tissue, CD34+ cells, astrocytes, neurons, and endothelial cells. VEGF can be detected in both human plasma and serum samples, with higher levels found in serum due to its release from platelets. VEGF transcription is potentiated in response to hypoxia, oncogenic transformation, and growth factors.\u003cbr\u003e\u003cbr\u003eVEGF has several physiological roles both during development and in the adult. It has a well-documented and critical role in embryonic vasculogenesis. Targeted deletion of a single VEGF allele results in significant defects in vascular development and is embryonic lethal. VEGF acts as an endothelial cell survival factor, mitogen, and inducer of migration, functions that potentially involve an array of signaling molecules including G proteins, PI3 kinase, MAP kinases, FAK, PKC, and NO. VEGF affects bone formation by regulating blood vessel growth and cartilage remodeling, activities important for growth plate morphogenesis. In the adult, VEGF has potential roles in cyclic angiogenesis in the female reproductive system. Other diverse functions include the regulation of hematopoietic development and roles as a neurotrophic factor.\u003cbr\u003e\u003cbr\u003eVEGF has received much attention for its involvement in tumor-associated angiogenesis and its potential as a target for cancer therapy. Numerous studies have demonstrated that VEGF or its receptors are upregulated in several forms of human cancer. Neutralizing VEGF antibodies are shown to reduce tumor growth in cancer models.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/7422\" target=\"_blank\"\u003e7422\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P15692\" target=\"_blank\"\u003eP15692\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eVPF; VEGF; MVCD1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to human VEGF (8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman VEGF Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-human VEGF monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e31.25 - 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant human VEGF. The factors listed below were prepared at 200ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eEC-VEGF\/PK1, PDGF-AA, PDGF-AB , PDGF-BB, PιGF, VEGF-B167, VEGF-C, VEGF-D, VEGF R3(Flt-4) \/Fc Chimera\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003ePιGF-2, VEGF120, VEGF164, VEGF-D, VEGF R2(KDR)\/Fc Chimera, VEGF R3(Flt-4) \/Fc Chimera\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther proteins\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eRat VEGF 120(0.07%), zebrafish VEGF 165\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-vegf-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-vegf-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKH1024\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-human-vegf-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-human-vegf-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099330099,"sku":"BSKH1024-96T","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskm1018","title":"Mouse VEGF ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for VEGF is added to the wells and binds to the combination of capture antibody-VEGF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of VEGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven VEGF standard dilutions and VEGF sample concentration determined.\u003cbr\u003e\u003cbr\u003eFor Use with serum, plasma and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/image002.jpg?v=1564166747\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eVascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adults. It is a member of the platelet derived growth factor family that is characterized by a cysteine knot structure formed by eight conserved cysteine residues. Mouse embryos expressing only the VEGF120 isoform do not survive to term and show defects in skeletogenesis. Mouse VEGF120 shares 98% aa sequence identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt 1) and VEGF R2 (Flk1\/KDR) on endothelial cells. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-α.\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGENE ID\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/22339\" target=\"_blank\"\u003e22339\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q00731\" target=\"_blank\"\u003eQ00731\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eVpf; Vegf\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse VEGF(8﹡12)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse VEGF Standard lyophilized, 2000 pg\/ml upon reconstitution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Biotin-Conjugate anti-mouse VEGF monoclonal antibody\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin-Conjugate antibody Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStreptavidin-HRP Diluent\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay were less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 7pg\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd\u003e15.6-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003eThis assay recognizes both natural and recombinant mouse VEGF. The factors listed below were prepared at 50ng\/ml in Standard \/sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eFactors assayed for cross-reactivity\u003c\/h5\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant mouse\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eG-CSF, GM-CSF, IL-1α, IL-1γ, IL-2 , IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, LIF, TNF-α, IFN-γ, MIP-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecombinant human\u003c\/td\u003e\n\u003ctd class=\"header\"\u003eFIt-3 Ligand, PιGF, PDGF-AA, PDGF-AB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-vegf-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-vegf-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for\u003cspan\u003e BSKM1018\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-mouse-vegf-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-mouse-vegf-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31740099362867,"sku":"BSKM1018","price":424.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskv0001","title":"SARS-CoV-2 Nucleoprotein ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for SARS-CoV-2 Nucleoprotein has been pre-coated onto a microplate. Standards and samples are pipetted into the wells, and then a horseradish peroxidase-conjugated detection antibody specific for SARS-CoV-2 Nucleoprotein is added to the wells,\u003c\/span\u003e \u003cspan style=\"font-weight: 400;\"\u003eproducing an antibody-antigen-antibody \"sandwich complex\". Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of SARS-CoV-2 Nucleoprotein present in the sample. The reaction is terminated by the addition of acid and absorbance is measured at 450nm. A standard curve is prepared from six SARS-CoV-2 Nucleoprotein standard dilutions and SARS-CoV-2 Nucleoprotein sample concentration determined.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/BSKV0001_Graphic.JPG?v=1586522204\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003cp\u003eCoronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.\u003c\/p\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminum pouches with a Microwell Plate coated with monoclonal antibody to SARS-CoV-2 Nucleoprotein (8×12)\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSARS-CoV-2 Nucleoprotein standard, 25ng\/ml \u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Detection antibody conjugated to horseradish peroxidase (HRP) \u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard \/sample Diluent\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection antibody Diluent \u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer Concentrate 20x (PBS with 1% Tween-20)\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eThe coefficient of variation of both intra-assay and inter-assay was less than 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd\u003eThe minimum detectable dose was 0.4ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003e\u003cspan style=\"font-weight: 400;\"\u003eThis assay can recognize both recombinant and natural SARS-CoV-2 Nucleoprotein，but will not react with recombinant MERS-CoV Nucleoprotein.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with \u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-nucleoprotein-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by \u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-nucleoprotein-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e specifically for bskv0001.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-nucleoprotein-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003ehttps:\/\/www.myassays.com\/bioss-sars-cov-2-nucleoprotein-elisa-kit.assay\u003c\/a\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31793530208307,"sku":"BSKV0001","price":585.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskv0002","title":"SARS-CoV-2 IgM Antibody ELISA Kit","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e The kit uses enzyme-linked immunoassay. According to the structural characteristics of IgM antibodies, the same protein antigen was selected to coat the microporous plate and to be labeled with horseradish peroxidase (HRP); Labeled SARS-CoV-2-n recombinant protein (labeled antigen) with horseradish peroxidase (HRP). The kit design is as follows:\u003c\/p\u003e\n\u003cp\u003eThe 96-well microporous plate is coated with a recombinant SARS-CoV-2-n protein (solid-phase antigen); When the positive sample is to be tested (IgM) and the labeled antigen is added to the microplate, the pattern and product of (solid antigen +IgMAb+ labeled antigen) → (solid antigen -IgMAb-4 labeled antigen) will be formed. Absorbance was measured at 450nm wavelength by a microplate reader. The concentration of IgM antibody in the sample is directly proportional to the absorbance (OD value).\u003c\/p\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/bskv0002_Graphic.JPG?v=1588281310\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e The new coronavirus belongs to the beta coronavirus of the genus β, which has an envelope, the particles are round or oval, often polymorphic, and the diameter is 60-140nm. Its genetic characteristics are significantly different from SARS-CoV and MERS-CoV. Current research shows that it has more than 85% homology with bat SARS-like coronavirus (bat-SLCOVZC45).\u003c\/p\u003e\n\u003cp\u003eIn vitro isolation and culture, SARS-CoV-2  can be found in human respiratory epithelial cells in about 96 hours, while it takes about 6 days to isolate and culture in Vero E6 and Huh-7 cell lines. Based on current epidemiological investigations, the incubation period is generally 7 days, with a maximum of 14 days. The main symptoms are fever, fatigue, and dry cough. A few patients have symptoms such as nasal congestion, runny nose, and diarrhea. In severe cases, dyspnea occurs more than a week later. In other severe cases, acute respiratory distress syndrome, septic shock, difficult to correct metabolic acidosis, and coagulation dysfunction develop rapidly. It is worth noting that in the course of severe and critically ill patients, there may be moderate to low fever, even without obvious fever. Some patients showed only low fever, mild fatigue, and no pneumonia and recovered after 1 week. In the early stages of the disease, the total number of white blood cells in the peripheral blood was normal or decreased, the lymphocyte count decreased, and some patients had increased liver enzymes, muscle enzymes, and myoglobin. Most patients have elevated C-reactive protein (CRP) and erythrocyte sedimentation rate and normal procalcitonin. In severe cases, D-dimer increases and peripheral blood lymphocytes progressively decrease. New coronavirus nucleic acids can be detected in throat swabs, sputum, lower respiratory tract secretions, and blood.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMicroporous plate coated with SARS-CoV-2-N recombinant protein antigen. (8×12)\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan style=\"font-weight: 400;\"\u003eIgM antibody negative reference: specification : 0.8ml\/ bottle\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan style=\"font-weight: 400;\"\u003eIgM antibody positive reference: specification : 0.8ml\/ bottle\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme conjugate: SARS-CoV-2-n antigen with HRP labeled:  specification: 10.5ml\/ bottle.\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor rendering solution A, the main component is H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e.  specification: 5.0ml\/ bottle.\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor rendering solution B, the main component is TMB.  specification: 5.0ml\/ bottle.\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTermination solution, mainly containing dilute H\u003csub\u003e2\u003c\/sub\u003eSO\u003csub\u003e4\u003c\/sub\u003e. \u003cbr\u003especification : 5.0ml\/ bottle.\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated washing solution, 10 times concentrated washing solution, dilute before use. Specification: 50.0ml\/ bottle.\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdhesive Films\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e4 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOperating Instructions\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003eTested the positive reference products of the enterprise. Repeated detection 10 times, all the results were positive.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003e\u003cspan style=\"font-weight: 400;\"\u003e\u003cspan\u003eAfter the destruction test of 2-mercapto ethanol in this kit; it is verified that the detected antibody is IgM type antibody\u003c\/span\u003e.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-igm-antibody-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-igm-antibody-elisa-kit.assay\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for bskv0002.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-igm-antibody-elisa-kit.assay\"\u003ehttps:\/\/www.myassays.com\/bioss-sars-cov-2-igm-antibody-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31887668445235,"sku":"BSKV0002","price":585.0,"currency_code":"USD","in_stock":true}]},{"product_id":"bskv0003","title":"SARS-CoV-2 Total Antibody Detection ELISA Kit (Targeting Nucleocapsid Proteins)","description":"\u003ch3\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eThe kit uses enzyme-linked immunoassay. According to the principle of antibody blocking; the following design was made: The recombinant protein of SARS-CoV-2-N was used as a coating antigen and was coated with a 96-well reaction plate. When the positive sample is added and within a certain reaction time; the (solid-phase antigen-antibody) complex is formed. Wash the plate; get rid of the substance that didn't participate in the reaction. HRP anti-SARS-CoV-2-N antibody was added as a tracer. Antigenic reaction with no binding antibody on the reaction plate; Formation of (Solid-phase antigen - HRP antibody) complexes. Absorbance was measured 450nm using a microplate reader. The concentration of specific total antibody in the sample was inversely proportional to the absorbance (OD value).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"image\"\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/files\/bskv0003_Graphic.JPG?v=1588281310\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eTarget Information\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by infection with the SARS-CoV-2 virus. Common signs of infection include respiratory symptoms, fever, cough, shortness of breath and breathing difficulties. In severe cases, the infection can cause pneumonia, severe acute respiratory syndrome (SARS), kidney failure and death. Coronaviruses (CoV) are a large family of viruses that cause illness ranging from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARS-CoV). SARS-CoV-2 is a new strain of coronavirus that was first identified in December 2019 when the first outbreak occurred in Wuhan, China.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003ch3\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Components\u003c\/th\u003e\n\u003cth class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMicroplate coated with SARS-CoV-2-N recombinant protein antigen. (8×12)\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan style=\"font-weight: 400;\"\u003eNegative reference product; Specification: 0.8ml \/ bottle\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan style=\"font-weight: 400;\"\u003ePositive reference product;  Specification: 0.8ml \/ bottle\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme Conjugate: anti-SARS-CoV-2-N monoclonal antibody with HRP marker: Specification: 10.5ml \/ bottle.\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor rendering solution A, mainly composed of H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e; Specification: 5.0ml \/ bottle.\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor rendering solution B, mainly composed of which is TMB. Specification: 5.0ml \/ bottle.\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTermination solution, mainly containing dilute H\u003csub\u003e2\u003c\/sub\u003eSO\u003csub\u003e4\u003c\/sub\u003e. Specification: 5.0ml \/ bottle.\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated washing solution, 10 times concentrated washing solution, diluted before use. Specification: 50.0ml \/ bottle.\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOperating instruction\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore at 2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd\u003e\u003cspan style=\"font-weight: 400;\"\u003eTested the positive reference products of the enterprise. Repeated detection 10 times, all the results were positive.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan style=\"font-weight: 400;\"\u003eReview the datasheet for extended details.\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eData Analysis Assistance\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eWe have partnered with\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-total-antibody-elisa-kit.assay\" target=\"_blank\"\u003eMyAssays\u003c\/a\u003e to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-total-antibody-elisa-kit.assay\" target=\"_blank\"\u003eMyAssays\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003especifically for bskv0003.\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/www.myassays.com\/bioss-sars-cov-2-total-antibody-elisa-kit.assay\" target=\"_blank\"\u003ehttps:\/\/www.myassays.com\/bioss-sars-cov-2-total-antibody-elisa-kit.assay\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Bioss","offers":[{"title":"96 tests","offer_id":31887668478003,"sku":"BSKV0003","price":585.0,"currency_code":"USD","in_stock":true}]}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1223\/3460\/collections\/iggy_smelling_flowers_-_Copy.jpg?v=1683143016","url":"https:\/\/www.biossusa.com\/collections\/elisa-kits.oembed?page=10","provider":"Bioss","version":"1.0","type":"link"}