MGMT (4C12) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-51234M
Product Name MGMT (4C12) Monoclonal Antibody
Applications WB, IHC-P, IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Purified His-tagged MGMT protein was used to produced this monoclonal antibody.
Clonality Monoclonal
Clone # 4C12
Isotype IgG1
Concentration 0.5ug/ul
Purification Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4255
Swiss Prot P16455
Synonyms Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Background Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF