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RAGE (8G4) Monoclonal Antibody

Flow cytometric analysis of Jurkat cells with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Flow cytometric analysis of Jurkat cells with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: mouse lung lysates; Probed with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: mouse lung lysates; Probed with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with RAGE (8G4) Monoclonal Antibody (bsm-52809R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Lung lysates; Lane 2: Rat Lung lysates probed with RAGE (8G4) Monoclonal Antibody, Unconjugated (bsm-52809R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Rat Lung lysates probed with RAGE (8G4) Monoclonal Antibody, Unconjugated (bsm-52809R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • FCM
  • IHC-P
  • IF(ICC)
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52809R
Product Name RAGE (8G4) Monoclonal Antibody
Applications WB, FCM, IHC-P, IF(ICC), IHC
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Human RAGE between 1 to 350 amino acids
Clonality Monoclonal
Clone # 8G4
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 177
Swiss Prot Q15109
Synonyms Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER
Background Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC

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