PDGFR alpha (5A1) Monoclonal Antibody

$Lane 1: NIH-3T3 Cells; Probed with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: NIH-3T3 Cells; Probed with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:100 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:100 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Uterus tissue incubated with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Uterus tissue incubated with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Flow cytometric analysis of PDGFRA was done on NIH\/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody PDGFRA Monoclonal Antibody (bsm-52829R) at a 1:50 dilution (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.(cells without incubation with primary antibody; black).$

Flow cytometric analysis of PDGFRA was done on NIH\/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody PDGFRA Monoclonal Antibody (bsm-52829R) at a 1:50 dilution (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.(cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PDGFRA (5A1) Monoclonal Antibody (bsm-52829R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Lane 1: Mouse Uterus tissue lysates; Lane 2: Mouse Lung tissue lysates probed with PDGFRA (5A1) Monoclonal Antibody, Unconjugated (bsm-52829R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52829R
Product Name	PDGFR alpha (5A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within c-terminal human PDGFR Alpha
Clonality	Monoclonal
Clone #	5A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5156
Swiss Prot	P16234
Subcellular location	Cell membrane, Golgi apparatus
Synonyms	Alpha-type platelet-derived growth factor receptor antibody;CD140 antigen-like family member A antibody;CD140a antibody;CD140a antigen antibody;MGC74795 antibody;PDGF alpha chain antibody;PDGF-R-alpha antibody;PDGFR 2 antibody;PDGFR alpha antibody;PDGFR2 antibody;PDGFRA antibody;PDGFRA/BCR fusion antibody;PGFRA_HUMAN antibody;Platelet derived growth factor receptor 2 antibody;Platelet derived growth factor receptor alpha antibody;Platelet derived growth factor receptor alpha polypeptide antibody;Platelet derived growth factor receptor antibody;Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody;RHEPDGFRA antibody
Background	Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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