Gelsolin Monoclonal Antibody

$Lane 1: Mouse Thymus; Lane 2: Mouse Lung; Lane 3: THP-1 Cells; Lane 4: HepG2 Cells; Probed with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Thymus; Lane 2: Mouse Lung; Lane 3: THP-1 Cells; Lane 4: HepG2 Cells; Probed with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of THP-1 cells with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Gelsolin (2G6) Monoclonal Antibody, Unconjugated (bsm-54290R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Gelsolin (2G6) Monoclonal Antibody, Unconjugated (bsm-54290R) at 1:200 overnight at 4\u00b0C, DAB staining.

Lane 1: Human HepG2 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human Siha cell lysates probed with Gelsolin Monoclonal Antibody, Unconjugated (bsm-54290R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Blocking buffer: 5% NFDM\/TBST\t \r\nPrimary Ab dilution: 1:2000\t \r\nPrimary Ab incubation condition: 2 hours at\t \r\nroom temperature\t \r\nSecondary Ab: Goat Anti-Rabbit IgG H&L\t \r\n(HRP)\t \r\nLysate: Human plasma\t \r\nProtein loading quantity: 20 \u6e2dg\t \r\nExposure time: 30 s\t \r\nPredicted MW: 81 kDa\t \r\nObserved MW: 81 kDa\t \r\n$

Blocking buffer: 5% NFDM\/TBST\t \r\nPrimary Ab dilution: 1:2000\t \r\nPrimary Ab incubation condition: 2 hours at\t \r\nroom temperature\t \r\nSecondary Ab: Goat Anti-Rabbit IgG H&L\t \r\n(HRP)\t \r\nLysate: Human plasma\t \r\nProtein loading quantity: 20 \u6e2dg\t \r\nExposure time: 30 s\t \r\nPredicted MW: 81 kDa\t \r\nObserved MW: 81 kDa\t \r\n

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54290R
Product Name	Gelsolin Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Gelsolin protein
Clonality	Monoclonal
Clone #	2G6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2934
Swiss Prot	P06396
Subcellular location	Cytoplasm, Secreted
Synonyms	Actin depolymerizing factor; ADF; AGEL; Amyloidosis Finnish type; Brevin; DKFZp313L0718; GSN; GELS_HUMAN; Actin-depolymerizing factor.
Background	Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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