TNF alpha Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)

Reactivity

  • Human
  • Mouse
  • Rat
  • Rabbit
  • Horse
Overview
Catalog # bs-2081R-A647
Product Name TNF alpha Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F)
Reactivity Human, Mouse, Rat, Rabbit, Horse
Specifications
Conjugation ALEXA FLUOR® 647
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse TNF-alpha
Immunogen Range 181-235/235
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 21926
Swiss Prot P06804
Subcellular location Secreted, Cell membrane
Synonyms DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; Tnf
Background Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200