25% Off Flow Cytometry Antibodies - Use Code: "EggyIggy2024" - Valid 3/29-4/5/24 for U.S. customers only

IRF3 (Ser396) Polyclonal Antibody, ALEXA FLUOR® 750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Monkey

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-3195R-A750
Product Name IRF3 (Ser396) Polyclonal Antibody, ALEXA FLUOR® 750 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat, Monkey
Predicted Reactivity Dog, Cow, Sheep, Pig
Specifications
Conjugation ALEXA FLUOR® 750
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396
Modification Site Ser396
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3661
Swiss Prot Q14653
Subcellular location Cytoplasm, Nucleus
Synonyms Interferon regulatory factor 3; IRF-3; IRF3
Background Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200