Recombinant SARS-CoV-2 Spike Protein S1

CATALOG # bs41405P
PRODUCT NAME Recombinant SARS-CoV-2 Spike Protein S1
CROSS REACTIVE SPECIES DETAILS Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
CONJUGATION Unconjugated
SEQUENCE An E.coli expression system produces recombinant SARS-CoV-2 Spike Protein S1, and the target gene encoding amino acids 16-685 is expressed with His Tag.
TAG His tag

PURITY > 95% as determined by SDS-PAGE
FORMULATION Lyophilized from 0.22um filtered solution in PBS (pH 7.4). 5 % trehalose is added as a protectant
STORAGE Store at 2 to 8 °C for one week. Store at -20 to -80 °C for twelve months from the date of receipt. Avoid repeated freeze/thaw cycles.
BACKGROUND The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to specific receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be crucial in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor-binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains essential elements needed for membrane fusion. The S protein plays vital parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; the Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor-binding domain (RBD), leading to a higher mortality rate.
NOTE For research use only. Not for use in clinical diagnostic procedures.