Recombinant SARS-CoV-2 N protein C-fragment

OVERVIEW
CATALOG # bs41409P
PRODUCT NAME Recombinant SARS-CoV-2 N protein C-fragment
CROSS REACTIVE SPECIES DETAILS Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
CONJUGATION Unconjugated
SEQUENCE An E.coli expression system produces recombinant SARS-CoV-2 N protein C-fragment, and the target gene encoding amino acids 243-369 is expressed with N-His Tag.
EXPRESSION SYSTEM E.coli
TAG N-His
PREDICTED MOLECULAR MASS 42.6kDa
PURITY ≥95% as determined by SDS-PAGE
FORMULATION Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5 % trehalose is added as a protectant before lyophilization.
STORAGE Store at 2 to 8 °C for one week. Store at -20 to -80 °C for twelve months from the date of receipt.  Avoid repeated freeze/thaw cycles.
ENDOTOXIN LEVEL N/A
ACTIVITY ASSAY N/A
TARGET
ACCESSION # QHD43423.2
BACKGROUND Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
NOTE For research use only. Not for use in clinical diagnostic procedures.