AIF Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Pig

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Chicken
  • Rabbit
Overview
Catalog # bs-0037R-A594
Product Name AIF Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
Applications IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat, Pig
Predicted Reactivity Dog, Cow, Sheep, Chicken, Rabbit
Specifications
Conjugation ALEXA FLUOR® 594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AIF
Immunogen Range 131-230/613
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles
Target
Gene ID 9131
Swiss Prot O95831
Subcellular location Cytoplasm, Nucleus
Synonyms AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6; Apoptosis-inducing factor 1, mitochondrial; Programmed cell death protein 8; AIFM1
Background Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200