Caspase 8 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated

Applications

  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-0052R-A555
Product Name Caspase 8 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated
Applications IF(IHC-P), IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation ALEXA FLUOR® 555
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Caspase-8 subunit p10
Immunogen Range 430-482/482
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Target
Gene ID 12370
Swiss Prot O89110
Subcellular location Cytoplasm
Synonyms MACH; Mch5; FLICE; CASP-8; Caspase-8; Casp8
Background Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
Application Dilution
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200