HIV1 gp120 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Virus
Overview
Catalog # bs-0241R
Product Name HIV1 gp120 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IF(IHC-P)
Reactivity Virus
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from HIV1 Surface protein gp120
Immunogen Range 290-340/857
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Swiss Prot P05877
Subcellular location Extracellular
Synonyms HIV-1 ENV gp120; HIV1 gp120; HIV1gp120; HIV-1 gp120; Envelope surface glycoprotein gp120; Glycoprotein 120; gp120; gp120 glycoprotein; Human Immunodeficiency Virus 1; SU; Surface protein; ENV_HV1MN; Envelope glycoprotein gp160; Env polyprotein; Surface protein gp120; SU.
Background The attachment of enveloped viruses to cells and the fusion of viral and cellular membranes are critical early events in the HIV viral infection. This process is mediated by envelope glycoproteins (gp) on the surface of the virus. The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein, gp160, is proteolytically cleaved into gp120 and gp41, which remain noncovalently associated with one another. gp120 is one of the proteins that forms the envelope of HIV. gp120 projects from the surface of HIV and binds to the CD4 molecule on helper T cells. gp120 has been a logical experimental HIV vaccine because the outer envelope is the first part of the virus that encounters antibody. gp41 is embedded in the outer envelope of HIV that anchors gp120. gp41 also plays a key role in HIV's infection of CD4+ T cells by facilitating the fusion of the viral and cell membranes. The nomenclature of the gp proteins describes their respective molecular masses (e.g., gp160, gp120, gp41).
Application Dilution
WB 1:300-1000
ELISA 1:500-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200