MrpL28 Polyclonal Antibody, Cy3 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
  • Chicken
Overview
Catalog # bs-0818R-Cy3
Product Name MrpL28 Polyclonal Antibody, Cy3 Conjugated
Applications IF(IHC-P), IF(IHC-F), IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Cow, Chicken
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MrpL28
Immunogen Range 131-240/240
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10573
Subcellular location Cytoplasm
Synonyms RM28_HUMAN; 39S ribosomal protein L28 mitochondrial; 39S ribosomal protein L28 mitochondrial precursor; HGNC6756; L28mt ; MAAT 1; MAAT1; Melanoma antigen p15; Melanoma associated antigen recognised by cytotoxic T lymphocytes; Melanoma associated antigen recognized by T lymphocytes; MGC8499; Mitochondrial ribosomal protein L28; MRP L28; MRPL 28; P15.
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL28 is a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA A24 restricted fashion.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200