Adeno-Associated Virus Capsid Protein VP1 Antibody

Applications

  • WB
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Virus
Overview
Catalog # bs-10180R
Product Name Adeno-Associated Virus Capsid Protein VP1 Antibody
Applications WB, IHC-P, IF(IHC-P)
Specificity This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13
Reactivity Virus
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1
Immunogen Range 30-80/724
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Synonyms capsid protein Adeno-associated 5 virus; capsid protein [Adeno-associated virus - 5]; capsid protein AAV5; Parvovirus coat protein VP1; capsid protein.
Background Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus
Application Dilution
WB 1:300-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200