25% Off Flow Cytometry Antibodies - Use Code: "EggyIggy2024" - Valid 3/29-4/5/24 for U.S. customers only

VEGFR2 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated

Applications

  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
  • Rabbit

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-10412R-A647
Product Name VEGFR2 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
Applications FCM, IF(IHC-P)
Reactivity Human, Mouse, Rat, Rabbit
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation ALEXA FLUOR® 647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human VEGFR2
Immunogen Range 101-200/1356
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3791
Swiss Prot P35968
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms FLK1; CD309; VEGFR; VEGFR2; Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1
Background Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200