TUG/ASPC Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-12355R-A488
Product Name TUG/ASPC Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation ALEXA FLUOR® 488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TUG/ASPC
Immunogen Range 351-420/553
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 79058
Subcellular location Cell membrane
Synonyms Alveolar soft part sarcoma chromosomal region candidate gene 1 protein; Alveolar soft part sarcoma chromosome region candidate 1 human; Alveolar soft part sarcoma locus; ASPC; ASPC1_HUMAN; ASPCR 1; ASPCR1; ASPL; ASPS; ASPSCR 1; Aspscr1; FLJ45380; RCC 17; RCC17; renal cell carcinoma gene on chromosome 17; renal cell carcinoma papillary 17; Renal papillary cell carcinoma protein 17; Tether containing a UBX domain for GLUT4; Tether containing UBX domain for GLUT4; TUG; UBX domain containing protein 9; UBX domain protein 9; UBX domain-containing protein 9; UBXD 9; UBXD9; UBXN 9; UBXN9.
Background Glut4 is a twelve pass transmembrane protein (12TM) whose carboxy-terminus may dictate its cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity. TUG (ASPL in humans) regulates the trafficking of glucose via Glut4. Full-length TUG forms a complex with Glut4 and in 3T3-L1 adipocytes and this complex is present in unstimulated cells and is disassembled by insulin. TUG acts by trapping endocytosed Glut4 and tethering it intracellularly. Insulin mobilizes this pool of retained Glut4 by releasing the tether.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200