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ATG4B Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse liver labeled with Anti-APG4B\/AUTL1 Polyclonal Antibody, Unconjugated (bs-1384R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse liver labeled with Anti-APG4B\/AUTL1 Polyclonal Antibody, Unconjugated (bs-1384R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Image kindly submitted by M. G. Connor as part of the free sample program. The bacteria Zprojection image figure legend: RAW264.7 Macrophage cells infected with GFP-bacteria (MOI3), stained with ATG4B primary antibody (1:1000) and visualized with Alexa Flour 595 (red). Nucleus is stained with DAPI (blue). Coverslips mounted with Prolong Gold Antifade with DAPI. Zprojections generated using FIJI. Scale bar is 5um. Images courtesy of M. G. Connor 2014
Image kindly submitted by M. G. Connor as part of the free sample program. The bacteria Zprojection image figure legend: RAW264.7 Macrophage cells infected with GFP-bacteria (MOI3), stained with ATG4B primary antibody (1:1000) and visualized with Alexa Flour 595 (red). Nucleus is stained with DAPI (blue). Coverslips mounted with Prolong Gold Antifade with DAPI. Zprojections generated using FIJI. Scale bar is 5um. Images courtesy of M. G. Connor 2014
U937 lysates probed with Rabbit Anti-ATG4B Polyclonal Antibody, Unconjugated (bs-1384R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
U937 lysates probed with Rabbit Anti-ATG4B Polyclonal Antibody, Unconjugated (bs-1384R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
Overview
Catalog # bs-1384R
Product Name ATG4B Polyclonal Antibody
Applications ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ATG4B
Immunogen Range 85-200/392
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 23192
Swiss Prot Q9Y4P1
Subcellular location Cytoplasm
Synonyms APG4B; AUTL1; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B; ATG4B; KIAA0943
Background Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

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