ACA11 Polyclonal Antibody, ALEXA FLUOR® 680 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Plant
Overview
Catalog # bs-2249R-A680
Product Name ACA11 Polyclonal Antibody, ALEXA FLUOR® 680 Conjugated
Applications IF(IHC-P), IF(IHC-F), IF(ICC)
Predicted Reactivity Plant
Specifications
Conjugation ALEXA FLUOR® 680
Host Rabbit
Source KLH conjugated synthetic peptide derived from Arabidopsis thaliana ACA11
Immunogen Range 201-300/1025
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 677770
Swiss Prot O22218
Subcellular location Cell membrane
Synonyms calcium-transporting ATPase; Putative calcium-transporting ATPase 11, plasma membrane-type; Ca2+-ATPase isoform 11; autoinhibited Ca2+-ATPase 11Arabidopsis thaliana; ACA11_ARATH.
Background In plant cells, the vacuole functions as a major calcium store. Although a calmodulin-regulated Ca2+-ATPase (ACA4) is known to be present in prevacuolar compartments, the presence of an ACA-type Ca2+-ATPase in the mature vacuole of a plant cell has not been verified. Here we provide evidence that ACA11 localizes to the vacuole membrane. ACA11 tagged with GFP was expressed in stable transgenic plants, and visualized in root cells and protoplasts by confocal microscopy. A Ca2+-ATPase function for ACA11 was confirmed by complementation of yeast mutants.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200