| Overview |
| bs-3185R-A350 |
| Histone H2A.X (Ser140) Antibody, ALEXA FLUOR® 350 Conjugated |
| WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC) |
| This phosphorylation site is homologous to that of Ser140 in Mouse and Rat. |
| Human, Others |
| Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications |
| ALEXA FLUOR® 350 |
| Rabbit |
| KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140 |
| Ser140 |
| Polyclonal |
| IgG |
| 1ug/ul |
| Purified by Protein A. |
| Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target |
| 3014 |
| P16104 |
| Nucleus |
| H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX |
| Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Application Dilution |
| WB |
1:300-5000 |
| FCM |
1:20-100 |
| IF(IHC-P) |
1:50-200 |
| IF(IHC-F) |
1:50-200 |
| IF(ICC) |
1:50-200 |
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