CD24 Polyclonal Antibody

Human A549 cells probed with CD24 Polyclonal Antibody (bs-4890R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG, FITC Conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue) and secondary only (light blue)

Human A549 cells probed with CD24 Polyclonal Antibody (bs-4890R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG, FITC Conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue) and secondary only (light blue)

A431 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD24 Polyclonal Antibody (bs-4890R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

A431 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD24 Polyclonal Antibody (bs-4890R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
ELISA
FCM

Reactivity

Human
Mouse

Overview
Catalog #	bs-4890R
Product Name	CD24 Polyclonal Antibody
Applications	WB, ELISA, FCM
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD24
Immunogen Range	27-59/80
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	100133941
Swiss Prot	P25063
Subcellular location	Cell membrane
Synonyms	CD24A; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; CD24
Background	Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
Application Dilution
WB	1:500-2000
ELISA	1:5000-10000
FCM	1:50