E2F1 (Ser332) Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated

Applications

WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bs-5306R-A350
Product Name	E2F1 (Ser332) Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
Applications	WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	ALEXA FLUOR® 350
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of Ser332
Modification Site	Ser332
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	1869
Subcellular location	Nucleus
Synonyms	E2F1Ser332; p-E2F1 Ser332; E2F1 phospho S332; E2F 1; E2F transcription factor 1; E2F-1; E2f1 E2F transcription factor 1; KIAA4009; mKIAA4009; OTTHUMP00000030661; PBR 3; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP 3; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma associated protein 1; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1; E2F1_HUMAN.
Background	E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

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