Neutrophil Elastase Polyclonal Antibody

Mouse spinal cord lysates probed with Neutrophil Elastase Polyclonal Antibody, unconjugated (bs-6982R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Lane 1: HL-60 cell lysates probed with Neutrophil Elastase Polyclonal Antibody, Unconjugated (bs-7537R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Mouse Bone tissue lysates; Lane 2: Rat Bone tissue lysates probed with Neutrophil Elastase Polyclonal Antibody, Unconjugated (bs-6982R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Neutrophil Elastase Polyclonal Antibody(bs-6982R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).