EMR1 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-7058R-A488
Product Name EMR1 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated
Applications WB, FCM, IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation ALEXA FLUOR® 488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EMR1
Immunogen Range 732-747/886
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2015
Swiss Prot Q14246
Subcellular location Extracellular
Synonyms TM7LN3; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; EMR1; Adhesion G Protein-Coupled Receptor E1; ADGRE1; Cell surface glycoprotein F4/80
Background The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (13). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (13). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200