{"product_id":"bskh64262","title":"Human Sialidase 2, Cytosolic (SIAL2) ELISA Kit","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe microtiter plate provided in this kit has been pre-coated with an antibody specific to SIAL2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SIAL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain SIAL2, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SIAL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003eFor Use with serum, plasma, tissue homogenates or other biological fluids. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\" class=\"image\"\u003e\u003c\/p\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eTarget Information\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003eExo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides (PubMed:14613940, PubMed:22228546). Recognizes sialyl linkage positions of the glycan moiety as well as the supramolecular organization of the sialoglycoconjugate. Displays preference for alpha-(2-\u0026gt;3)-sialylated GD1a and GT1B gangliosides over alpha-(2-\u0026gt;8)-sialylated GD1b, in both monomeric forms and micelles. Hydrolyzes monomeric GM1 ganglioside, but has no activity toward the miscellar form (PubMed:14613940). Has lower sialidase activity for glycoproteins such as fetuin and TF\/transferrin that carry a mixture of alpha-(2-\u0026gt;3) and alpha-(2-\u0026gt;6)-sialyl linkages. Cleaves milk oligosaccharide alpha-(2-\u0026gt;3)-sialyllactose, but is inactive toward alpha-(2-\u0026gt;6)-sialyllactose isomer. Has no activity toward colominic acid, a homomer of alpha-(2-\u0026gt;8)-linked Neu5Ac residues (PubMed:14613940).\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eGENE ID\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/4759\" title=\"4759\" target=\"_blank\" rel=\"noopener noreferrer\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/4759\"\u003e4759\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q9Y3R4\" title=\"Q9Y3R4\" target=\"_blank\" rel=\"noopener noreferrer\" data-mce-href=\"https:\/\/www.uniprot.org\/uniprot\/Q9Y3R4\"\u003eQ9Y3R4\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eNEU2; N-Acetyl-Alpha-Neuraminidase 2; Cytosolic sialidase\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003e\n\u003cbr\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003eKit Components\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePre-coated, ready-to-use 96-well strip plate\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePlate sealer for 96 wells\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eStandard\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e2 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDiluent buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDetection Reagent A\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDetection Reagent B\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eWash Buffer (30 ℅ concentrate)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eStorage\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThe TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level SIAL2 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level SIAL2 were tested on 3 different plates, with 8 replicates in each plate.\u003cbr\u003eCV(%) = SD\/meanX100\u003cbr\u003e\u003cbr\u003eIntra-Assay: CV\u0026lt;10%\u003cbr\u003eInter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThe minimum detectable dose was 0.082ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.312-20ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis assay has high sensitivity and excellent specificity for the detection of SIAL2.\u003cbr\u003eNo significant cross-reactivity or interference between SIAL2 and analogs was observed.\u003cbr\u003eNote:\u003cbr\u003eLimited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between SIAL2 and all analogs, therefore, cross reactivity may still exist.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Bioss","offers":[{"title":"96T","offer_id":41092331503667,"sku":"BSKH64262-96T","price":650.0,"currency_code":"USD","in_stock":true},{"title":"48T","offer_id":41092331536435,"sku":"BSKH64262-48T","price":455.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.biossusa.com\/products\/bskh64262","provider":"Bioss","version":"1.0","type":"link"}