{"product_id":"bskh64270","title":"Human Sirtuin 7 (SIRT7) ELISA Kit","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRT7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SIRT7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain SIRT7, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SIRT7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003eFor Use with tissue homogenates, cell lysates, cell culture supernates or other biological fluids. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\" class=\"image\"\u003e\u003c\/p\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eTarget Information\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003eNAD-dependent protein-lysine deacylase that can act both as a deacetylase or deacylase (desuccinylase, depropionylase and deglutarylase), depending on the context (PubMed:22722849, PubMed:26907567, PubMed:30653310, PubMed:31542297). Specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac) (PubMed:22722849, PubMed:30420520). In contrast to other histone deacetylases, displays strong preference for a specific histone mark, H3K18Ac, directly linked to control of gene expression (PubMed:22722849, PubMed:30653310). H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors; SIRT7 thereby acts as a transcription repressor (PubMed:22722849). Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells (PubMed:22722849). Also able to mediate deacetylation of histone H3 at 'Lys-36' (H3K36Ac) in the context of nucleosomes (PubMed:30653310). Also mediates deacetylation of non-histone proteins, such as ATM, CDK9, DDX21, DDB1, FBL, FKBP5\/FKBP51, GABPB1, RAN, RRP9\/U3-55K and POLR1E\/PAF53 (PubMed:24207024, PubMed:26867678, PubMed:28147277, PubMed:28886238, PubMed:28426094, PubMed:30540930, PubMed:31075303, PubMed:30944854, PubMed:28790157). Enriched in nucleolus where it stimulates transcription activity of the RNA polymerase I complex (PubMed:16618798, PubMed:19174463, PubMed:24207024). Acts by mediating the deacetylation of the RNA polymerase I subunit POLR1E\/PAF53, thereby promoting the association of RNA polymerase I with the rDNA promoter region and coding region (PubMed:16618798, PubMed:19174463, PubMed:24207024). In response to metabolic stress, SIRT7 is released from nucleoli leading to hyperacetylation of POLR1E\/PAF53 and decreased RNA polymerase I transcription (PubMed:24207024). Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis (PubMed:19174463). Promotes pre-ribosomal RNA (pre-rRNA) cleavage at the 5'-terminal processing site by mediating deacetylation of RRP9\/U3-55K, a core subunit of the U3 snoRNP complex (PubMed:26867678). Mediates 'Lys-37' deacetylation of Ran, thereby regulating the nuclear export of NF-kappa-B subunit RELA\/p65 (PubMed:31075303). Acts as a regulator of DNA damage repair by mediating deacetylation of ATM during the late stages of DNA damage response, promoting ATM dephosphorylation and deactivation (PubMed:30944854). Suppresses the activity of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes by mediating deacetylation of DDB1, which prevents the interaction between DDB1 and CUL4 (CUL4A or CUL4B) (PubMed:28886238). Activates RNA polymerase II transcription by mediating deacetylation of CDK9, thereby promoting 'Ser-2' phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (PubMed:28426094). Deacetylates FBL, promoting histone-glutamine methyltransferase activity of FBL (PubMed:30540930). Acts as a regulator of mitochondrial function by catalyzing deacetylation of GABPB1 (By similarity). Regulates Akt\/AKT1 activity by mediating deacetylation of FKBP5\/FKBP51 (PubMed:28147277). Required to prevent R-loop-associated DNA damage and transcription-associated genomic instability by mediating deacetylation and subsequent activation of DDX21, thereby overcoming R-loop-mediated stalling of RNA polymerases (PubMed:28790157). In addition to protein deacetylase activity, also acts as protein-lysine deacylase (PubMed:27436229, PubMed:27997115, PubMed:31542297). Acts as a protein depropionylase by mediating depropionylation of Osterix (SP7), thereby regulating bone formation by osteoblasts (By similarity). Acts as a histone deglutarylase by mediating deglutarylation of histone H4 on 'Lys-91' (H4K91glu); a mark that destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes (PubMed:31542297). Acts as a histone desuccinylase: in response to DNA damage, recruited to DNA double-strand breaks (DSBs) and catalyzes desuccinylation of histone H3 on 'Lys-122' (H3K122succ), thereby promoting chromatin condensation and DSB repair (PubMed:27436229). Also promotes DSB repair by promoting H3K18Ac deacetylation, regulating non-homologous end joining (NHEJ) (By similarity). Along with its role in DNA repair, required for chromosome synapsis during prophase I of female meiosis by catalyzing H3K18Ac deacetylation (By similarity). Involved in transcriptional repression of LINE-1 retrotransposon via H3K18Ac deacetylation, and promotes their association with the nuclear lamina (PubMed:31226208). Required to stabilize ribosomal DNA (rDNA) heterochromatin and prevent cellular senescence induced by rDNA instability (PubMed:29728458). Acts as a negative regulator of SIRT1 by preventing autodeacetylation of SIRT1, restricting SIRT1 deacetylase activity (By similarity).\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eGENE ID\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/51547\" title=\"51547\" target=\"_blank\" rel=\"noopener noreferrer\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/51547\"\u003e51547\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q9NRC8\" title=\"Q9NRC8\" target=\"_blank\" rel=\"noopener noreferrer\" data-mce-href=\"https:\/\/www.uniprot.org\/uniprot\/Q9NRC8\"\u003eQ9NRC8\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eSIR2L7; NAD-Dependent Deacetylase Sirtuin-7; Silent Mating Type Information Regulation 2 Homolog 7; Regulatory protein SIR2 homolog 7; SIR2-like protein 7\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003e\n\u003cbr\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003eKit Components\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePre-coated, ready-to-use 96-well strip plate\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePlate sealer for 96 wells\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eStandard\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e2 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDiluent buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDetection Reagent A\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDetection Reagent B\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eWash Buffer (30 ℅ concentrate)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eStorage\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThe TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level SIRT7 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level SIRT7 were tested on 3 different plates, with 8 replicates in each plate.\u003cbr\u003eCV(%) = SD\/meanX100\u003cbr\u003e\u003cbr\u003eIntra-Assay: CV\u0026lt;10%\u003cbr\u003eInter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThe minimum detectable dose was 0.071ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.156-10ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis assay has high sensitivity and excellent specificity for the detection of SIRT7.\u003cbr\u003eNo significant cross-reactivity or interference between SIRT7 and analogs was observed.\u003cbr\u003eNote:\u003cbr\u003eLimited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between SIRT7 and all analogs, therefore, cross reactivity may still exist.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Bioss","offers":[{"title":"96T","offer_id":41092333109299,"sku":"BSKH64270-96T","price":650.0,"currency_code":"USD","in_stock":true},{"title":"48T","offer_id":41092333142067,"sku":"BSKH64270-48T","price":455.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.biossusa.com\/products\/bskh64270","provider":"Bioss","version":"1.0","type":"link"}