{"product_id":"bskm65014","title":"Mouse RAB5A, Member RAS Oncogene Family (RAB5A) ELISA Kit","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003ePrinciple of the Assay\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RAB5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain RAB5A, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAB5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003eFor Use with tissue homogenates, cell lysates or other biological fluids. For Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\" class=\"image\"\u003e\u003c\/p\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eTarget Information\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSmall GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (By similarity). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (PubMed:18425118).\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eGENE ID\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/271457\" title=\"271457\" target=\"_blank\" rel=\"noopener noreferrer\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/271457\"\u003e271457\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSWISS PROT\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q9CQD1\" title=\"Q9CQD1\" target=\"_blank\" rel=\"noopener noreferrer\" data-mce-href=\"https:\/\/www.uniprot.org\/uniprot\/Q9CQD1\"\u003eQ9CQD1\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSYNONYMS\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eRAB5\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003e\n\u003cbr\u003eMaterials Supplied\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003eKit Components\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" class=\"header\"\u003e96 Wells Quantity\/Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePre-coated, ready-to-use 96-well strip plate\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePlate sealer for 96 wells\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eStandard\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e2 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDiluent buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDetection Reagent A\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eDetection Reagent B\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eWash Buffer (30 ℅ concentrate)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eProduct data sheet\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eStorage\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThe TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003ePerformance Characteristics\u003c\/h3\u003e\n\u003ctable data-mce-fragment=\"1\" cellpadding=\"0\" cellspacing=\"0\" border=\"0\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eREPEATABILITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level RAB5A were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level RAB5A were tested on 3 different plates, with 8 replicates in each plate.\u003cbr\u003eCV(%) = SD\/meanX100\u003cbr\u003e\u003cbr\u003eIntra-Assay: CV\u0026lt;10%\u003cbr\u003eInter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eSENSITIVITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThe minimum detectable dose was 0.062ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eASSAY RANGE\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.156-10ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"header\"\u003eSPECIFICITY\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis assay has high sensitivity and excellent specificity for the detection of RAB5A.\u003cbr\u003eNo significant cross-reactivity or interference between RAB5A and analogs was observed.\u003cbr\u003eNote:\u003cbr\u003eLimited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between RAB5A and all analogs, therefore, cross reactivity may still exist.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Bioss","offers":[{"title":"96T","offer_id":41092303585331,"sku":"BSKM65014-96T","price":675.0,"currency_code":"USD","in_stock":true},{"title":"48T","offer_id":41092303618099,"sku":"BSKM65014-48T","price":472.5,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.biossusa.com\/products\/bskm65014","provider":"Bioss","version":"1.0","type":"link"}