HPV16 E6 protein (2E3) Monoclonal Antibody

Applications

  • IHC-P
  • IF(IHC-P)

Reactivity

  • Virus
Overview
Catalog # bsm-0990M
Product Name HPV16 E6 protein (2E3) Monoclonal Antibody
Applications IHC-P, IF(IHC-P)
Reactivity Virus
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from HPV16 E6 protein
Immunogen Range 85-158/158
Clonality Monoclonal
Clone # 2.00E+03
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID 1489078
Swiss Prot P03126
Synonyms Protein E6; E6
Background Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.
Application Dilution
IHC-P 1:200-400
IF(IHC-P) 1:50-200