MEK2 (1A1) Monoclonal Antibody

$Lane 1 : MEK1 Recombinant Protein; Lane 2 : MEK2 Recombinant Protein; Lane 3 : MEK3 Recombinant Protein; Lane 4 : MEK4 Recombinant Protein; Lane 5 : MEK5 Recombinant Protein; Lane 6 : MEK6 Recombinant Protein; Lane 7 : MEK7 Recombinant Protein; probed with MEK2 (1A1) Monoclonal Antibody (bsm-50250M) at 1.0ug\/mL (1:1000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1 : MEK1 Recombinant Protein; Lane 2 : MEK2 Recombinant Protein; Lane 3 : MEK3 Recombinant Protein; Lane 4 : MEK4 Recombinant Protein; Lane 5 : MEK5 Recombinant Protein; Lane 6 : MEK6 Recombinant Protein; Lane 7 : MEK7 Recombinant Protein; probed with MEK2 (1A1) Monoclonal Antibody (bsm-50250M) at 1.0ug\/mL (1:1000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

$Lane 1 : HeLa Cell Lysate; Lane 2 : U87mg Cell Lysate; Lane 3 : MCF7 Cell Lysate; Lane 4 : U937 Cell Lysate; probed with MEK2 (1A1) Monoclonal Antibody (bsm-50250M) at 2.0ug\/mL (1:500) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1 : HeLa Cell Lysate; Lane 2 : U87mg Cell Lysate; Lane 3 : MCF7 Cell Lysate; Lane 4 : U937 Cell Lysate; probed with MEK2 (1A1) Monoclonal Antibody (bsm-50250M) at 2.0ug\/mL (1:500) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human

Overview
Catalog #	bsm-50250M
Product Name	MEK2 (1A1) Monoclonal Antibody
Applications	WB, IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human protein purified from E.coli (His-MEK2)
Clonality	Monoclonal
Clone #	1A1
Isotype	IgG1
Concentration	Not Determined
Purification	Ammonium sulfate precipitation
Storage Buffer	HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Storage Condition	Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Target
Gene ID	5605
Swiss Prot	P36507
Synonyms	CFC4; MEK2; MKK2; MAPKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2; MAP2K2
Background	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Application Dilution
WB	1:300-1000
IP	1-2ug

Applications

Reactivity

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